Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. / Bernardo, Andreia S.; Jouneau, Alice; Marks, Hendrik; Kensche, Philip; Kobolak, Julianna; Freude, Kristine; Hall, Vanessa; Feher, Anita; Polgar, Zsuzsanna; Sartori, Chiara; Bock, Istvan; Louet, Claire; Faial, Tiago; Kerstens, Hindrik H. D.; Bouissou, Camille; Parsonage, Gregory; Mashayekhi, Kaveh; Smith, James C.; Lazzari, Giovanna; Hyttel, Poul; Stunnenberg, Hendrik G.; Huynen, Martijn; Pedersen, Roger A.; Dinnyes, Andras.

In: Biology Open, Vol. 7, No. 8, bio033282, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bernardo, AS, Jouneau, A, Marks, H, Kensche, P, Kobolak, J, Freude, K, Hall, V, Feher, A, Polgar, Z, Sartori, C, Bock, I, Louet, C, Faial, T, Kerstens, HHD, Bouissou, C, Parsonage, G, Mashayekhi, K, Smith, JC, Lazzari, G, Hyttel, P, Stunnenberg, HG, Huynen, M, Pedersen, RA & Dinnyes, A 2018, 'Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state', Biology Open, vol. 7, no. 8, bio033282. https://doi.org/10.1242/bio.033282

APA

Bernardo, A. S., Jouneau, A., Marks, H., Kensche, P., Kobolak, J., Freude, K., Hall, V., Feher, A., Polgar, Z., Sartori, C., Bock, I., Louet, C., Faial, T., Kerstens, H. H. D., Bouissou, C., Parsonage, G., Mashayekhi, K., Smith, J. C., Lazzari, G., ... Dinnyes, A. (2018). Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biology Open, 7(8), [bio033282]. https://doi.org/10.1242/bio.033282

Vancouver

Bernardo AS, Jouneau A, Marks H, Kensche P, Kobolak J, Freude K et al. Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biology Open. 2018;7(8). bio033282. https://doi.org/10.1242/bio.033282

Author

Bernardo, Andreia S. ; Jouneau, Alice ; Marks, Hendrik ; Kensche, Philip ; Kobolak, Julianna ; Freude, Kristine ; Hall, Vanessa ; Feher, Anita ; Polgar, Zsuzsanna ; Sartori, Chiara ; Bock, Istvan ; Louet, Claire ; Faial, Tiago ; Kerstens, Hindrik H. D. ; Bouissou, Camille ; Parsonage, Gregory ; Mashayekhi, Kaveh ; Smith, James C. ; Lazzari, Giovanna ; Hyttel, Poul ; Stunnenberg, Hendrik G. ; Huynen, Martijn ; Pedersen, Roger A. ; Dinnyes, Andras. / Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. In: Biology Open. 2018 ; Vol. 7, No. 8.

Bibtex

@article{e67f2d5cbf9749d1acc245b01fe2cafe,
title = "Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state",
abstract = "During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, {\textquoteleft}na{\"i}ve{\textquoteright} or {\textquoteleft}primed{\textquoteright}, depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states in vivo and in vitro. Amongst these are the pluripotency-linked genes Klf4 and Lin28b. The novel genes discovered include na{\"i}ve- (Spic, Scpep1 and Gjb5) and primed-associated (Sema6a and Jakmip2) genes as well as na{\"i}ve to primed transition genes (Dusp6 and Trip6). Both Gjb5 and Dusp6 play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.",
keywords = "Dusp6, Gjb5, Trip6, Naive, Pluripotency, Primed",
author = "Bernardo, {Andreia S.} and Alice Jouneau and Hendrik Marks and Philip Kensche and Julianna Kobolak and Kristine Freude and Vanessa Hall and Anita Feher and Zsuzsanna Polgar and Chiara Sartori and Istvan Bock and Claire Louet and Tiago Faial and Kerstens, {Hindrik H. D.} and Camille Bouissou and Gregory Parsonage and Kaveh Mashayekhi and Smith, {James C.} and Giovanna Lazzari and Poul Hyttel and Stunnenberg, {Hendrik G.} and Martijn Huynen and Pedersen, {Roger A.} and Andras Dinnyes",
year = "2018",
doi = "10.1242/bio.033282",
language = "English",
volume = "7",
journal = "Biology Open",
issn = "2046-6390",
publisher = "The Company of Biologists Ltd.",
number = "8",

}

RIS

TY - JOUR

T1 - Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state

AU - Bernardo, Andreia S.

AU - Jouneau, Alice

AU - Marks, Hendrik

AU - Kensche, Philip

AU - Kobolak, Julianna

AU - Freude, Kristine

AU - Hall, Vanessa

AU - Feher, Anita

AU - Polgar, Zsuzsanna

AU - Sartori, Chiara

AU - Bock, Istvan

AU - Louet, Claire

AU - Faial, Tiago

AU - Kerstens, Hindrik H. D.

AU - Bouissou, Camille

AU - Parsonage, Gregory

AU - Mashayekhi, Kaveh

AU - Smith, James C.

AU - Lazzari, Giovanna

AU - Hyttel, Poul

AU - Stunnenberg, Hendrik G.

AU - Huynen, Martijn

AU - Pedersen, Roger A.

AU - Dinnyes, Andras

PY - 2018

Y1 - 2018

N2 - During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, ‘naïve’ or ‘primed’, depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states in vivo and in vitro. Amongst these are the pluripotency-linked genes Klf4 and Lin28b. The novel genes discovered include naïve- (Spic, Scpep1 and Gjb5) and primed-associated (Sema6a and Jakmip2) genes as well as naïve to primed transition genes (Dusp6 and Trip6). Both Gjb5 and Dusp6 play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.

AB - During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, ‘naïve’ or ‘primed’, depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states in vivo and in vitro. Amongst these are the pluripotency-linked genes Klf4 and Lin28b. The novel genes discovered include naïve- (Spic, Scpep1 and Gjb5) and primed-associated (Sema6a and Jakmip2) genes as well as naïve to primed transition genes (Dusp6 and Trip6). Both Gjb5 and Dusp6 play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.

KW - Dusp6

KW - Gjb5

KW - Trip6

KW - Naive

KW - Pluripotency

KW - Primed

U2 - 10.1242/bio.033282

DO - 10.1242/bio.033282

M3 - Journal article

C2 - 30026265

VL - 7

JO - Biology Open

JF - Biology Open

SN - 2046-6390

IS - 8

M1 - bio033282

ER -

ID: 202340232