Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity

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Standard

Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity. / Bech-Nielsen, Gunilla Veslemöy; Hansen, Camilla Hartmann Friis; Hufeldt, Majbritt Ravn; Nielsen, Dennis Sandris; Aasted, Bent; Vogensen, Finn Kvist; Midtvedt, Tore; Hansen, Axel Jacob Kornerup.

In: Research in Veterinary Science, Vol. 92, No. 3, 12.06.2012, p. 501-508.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bech-Nielsen, GV, Hansen, CHF, Hufeldt, MR, Nielsen, DS, Aasted, B, Vogensen, FK, Midtvedt, T & Hansen, AJK 2012, 'Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity', Research in Veterinary Science, vol. 92, no. 3, pp. 501-508. https://doi.org/10.1016/j.rvsc.2011.04.005

APA

Bech-Nielsen, G. V., Hansen, C. H. F., Hufeldt, M. R., Nielsen, D. S., Aasted, B., Vogensen, F. K., Midtvedt, T., & Hansen, A. J. K. (2012). Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity. Research in Veterinary Science, 92(3), 501-508. https://doi.org/10.1016/j.rvsc.2011.04.005

Vancouver

Bech-Nielsen GV, Hansen CHF, Hufeldt MR, Nielsen DS, Aasted B, Vogensen FK et al. Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity. Research in Veterinary Science. 2012 Jun 12;92(3):501-508. https://doi.org/10.1016/j.rvsc.2011.04.005

Author

Bech-Nielsen, Gunilla Veslemöy ; Hansen, Camilla Hartmann Friis ; Hufeldt, Majbritt Ravn ; Nielsen, Dennis Sandris ; Aasted, Bent ; Vogensen, Finn Kvist ; Midtvedt, Tore ; Hansen, Axel Jacob Kornerup. / Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity. In: Research in Veterinary Science. 2012 ; Vol. 92, No. 3. pp. 501-508.

Bibtex

@article{add368705e2844688a14bf79774a7f2c,
title = "Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity",
abstract = "Inflammatory diseases such as type 2 diabetes (T2D) in humans and mice are under the influence of the composition of the gut microbiota (GM). It was previously demonstrated that treating Lepob mice with antibiotics improved glucose tolerance. However, wild type C57BL/6J mice may also exhibit plasma glucose intolerance reminiscent of human T2D. We hypothesized that antibiotic treatment in C57BL/6 mice would have an impact on glucose tolerance without affecting weight and gut immunology. When compared to mice treated with erythromycin or the controls, treatment for five weeks with ampicillin improved glucose tolerance without significantly affecting the weight or the number of gut mucosal regulatory T cells, tolerogenic dendritic cells or T helper cells type 1. 16S rRNA gene based denaturing gradient gel electrophoresis profiles clearly clustered according to treatment and showed that antibiotic treatment reduced GM diversity. It is concluded that antibiotic treatment changes glucose metabolism as well as the composition of the GM in C57BL/6 mice, and that this does not seem to be correlated to weight development in the mice.",
author = "Bech-Nielsen, {Gunilla Veslem{\"o}y} and Hansen, {Camilla Hartmann Friis} and Hufeldt, {Majbritt Ravn} and Nielsen, {Dennis Sandris} and Bent Aasted and Vogensen, {Finn Kvist} and Tore Midtvedt and Hansen, {Axel Jacob Kornerup}",
year = "2012",
month = jun,
day = "12",
doi = "10.1016/j.rvsc.2011.04.005",
language = "English",
volume = "92",
pages = "501--508",
journal = "Research in Veterinary Science",
issn = "0034-5288",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity

AU - Bech-Nielsen, Gunilla Veslemöy

AU - Hansen, Camilla Hartmann Friis

AU - Hufeldt, Majbritt Ravn

AU - Nielsen, Dennis Sandris

AU - Aasted, Bent

AU - Vogensen, Finn Kvist

AU - Midtvedt, Tore

AU - Hansen, Axel Jacob Kornerup

PY - 2012/6/12

Y1 - 2012/6/12

N2 - Inflammatory diseases such as type 2 diabetes (T2D) in humans and mice are under the influence of the composition of the gut microbiota (GM). It was previously demonstrated that treating Lepob mice with antibiotics improved glucose tolerance. However, wild type C57BL/6J mice may also exhibit plasma glucose intolerance reminiscent of human T2D. We hypothesized that antibiotic treatment in C57BL/6 mice would have an impact on glucose tolerance without affecting weight and gut immunology. When compared to mice treated with erythromycin or the controls, treatment for five weeks with ampicillin improved glucose tolerance without significantly affecting the weight or the number of gut mucosal regulatory T cells, tolerogenic dendritic cells or T helper cells type 1. 16S rRNA gene based denaturing gradient gel electrophoresis profiles clearly clustered according to treatment and showed that antibiotic treatment reduced GM diversity. It is concluded that antibiotic treatment changes glucose metabolism as well as the composition of the GM in C57BL/6 mice, and that this does not seem to be correlated to weight development in the mice.

AB - Inflammatory diseases such as type 2 diabetes (T2D) in humans and mice are under the influence of the composition of the gut microbiota (GM). It was previously demonstrated that treating Lepob mice with antibiotics improved glucose tolerance. However, wild type C57BL/6J mice may also exhibit plasma glucose intolerance reminiscent of human T2D. We hypothesized that antibiotic treatment in C57BL/6 mice would have an impact on glucose tolerance without affecting weight and gut immunology. When compared to mice treated with erythromycin or the controls, treatment for five weeks with ampicillin improved glucose tolerance without significantly affecting the weight or the number of gut mucosal regulatory T cells, tolerogenic dendritic cells or T helper cells type 1. 16S rRNA gene based denaturing gradient gel electrophoresis profiles clearly clustered according to treatment and showed that antibiotic treatment reduced GM diversity. It is concluded that antibiotic treatment changes glucose metabolism as well as the composition of the GM in C57BL/6 mice, and that this does not seem to be correlated to weight development in the mice.

U2 - 10.1016/j.rvsc.2011.04.005

DO - 10.1016/j.rvsc.2011.04.005

M3 - Journal article

C2 - 21543097

VL - 92

SP - 501

EP - 508

JO - Research in Veterinary Science

JF - Research in Veterinary Science

SN - 0034-5288

IS - 3

ER -

ID: 38105511