Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens. / Støy, Ann Cathrine Findal; Mølbak, Lars; Delègue, C. L.; Thymann, Thomas; Sangild, Per Torp; Heegaard, P. M. H.; Manurung, Sarmauli; Skovgaard, K.

In: Neonatology, Vol. 108, No. 3, 2015, p. 188-95.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Støy, ACF, Mølbak, L, Delègue, CL, Thymann, T, Sangild, PT, Heegaard, PMH, Manurung, S & Skovgaard, K 2015, 'Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens', Neonatology, vol. 108, no. 3, pp. 188-95. https://doi.org/10.1159/000431280

APA

Støy, A. C. F., Mølbak, L., Delègue, C. L., Thymann, T., Sangild, P. T., Heegaard, P. M. H., Manurung, S., & Skovgaard, K. (2015). Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens. Neonatology, 108(3), 188-95. https://doi.org/10.1159/000431280

Vancouver

Støy ACF, Mølbak L, Delègue CL, Thymann T, Sangild PT, Heegaard PMH et al. Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens. Neonatology. 2015;108(3):188-95. https://doi.org/10.1159/000431280

Author

Støy, Ann Cathrine Findal ; Mølbak, Lars ; Delègue, C. L. ; Thymann, Thomas ; Sangild, Per Torp ; Heegaard, P. M. H. ; Manurung, Sarmauli ; Skovgaard, K. / Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens. In: Neonatology. 2015 ; Vol. 108, No. 3. pp. 188-95.

Bibtex

@article{144fe5499aaf4b798d5fa6c5fd1265bd,
title = "Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens",
abstract = "BACKGROUND: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions.OBJECTIVE: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC.METHODS: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response.RESULTS: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.CONCLUSIONS: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.",
author = "St{\o}y, {Ann Cathrine Findal} and Lars M{\o}lbak and Del{\`e}gue, {C. L.} and Thomas Thymann and Sangild, {Per Torp} and Heegaard, {P. M. H.} and Sarmauli Manurung and K. Skovgaard",
note = "{\textcopyright} 2015 S. Karger AG, Basel.",
year = "2015",
doi = "10.1159/000431280",
language = "English",
volume = "108",
pages = "188--95",
journal = "Neonatology",
issn = "1661-7800",
publisher = "S Karger AG",
number = "3",

}

RIS

TY - JOUR

T1 - Necrotizing enterocolitis in preterm pigs is associated with increased density of intestinal mucosa-associated bacteria including Clostridium perfringens

AU - Støy, Ann Cathrine Findal

AU - Mølbak, Lars

AU - Delègue, C. L.

AU - Thymann, Thomas

AU - Sangild, Per Torp

AU - Heegaard, P. M. H.

AU - Manurung, Sarmauli

AU - Skovgaard, K.

N1 - © 2015 S. Karger AG, Basel.

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions.OBJECTIVE: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC.METHODS: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response.RESULTS: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.CONCLUSIONS: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.

AB - BACKGROUND: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions.OBJECTIVE: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC.METHODS: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response.RESULTS: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.CONCLUSIONS: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.

U2 - 10.1159/000431280

DO - 10.1159/000431280

M3 - Journal article

C2 - 26278554

VL - 108

SP - 188

EP - 195

JO - Neonatology

JF - Neonatology

SN - 1661-7800

IS - 3

ER -

ID: 159819580