Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain

Research output: Contribution to journal › Journal article › Research › peer-review

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Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain. / Prakash, Nilima; Puelles, Eduardo; Freude, Karla Kristine; Trümbach, Dietrich; Omodei, Daniela; Di Salvio, Michela; Sussel, Lori; Ericson, Johan; Sander, Maike; Simeone, Antonio; Wurst, Wolfgang.

In: Development (Cambridge, England), Vol. 136, No. 15, 2009, p. 2545-2555.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Prakash, N, Puelles, E, Freude, KK, Trümbach, D, Omodei, D, Di Salvio, M, Sussel, L, Ericson, J, Sander, M, Simeone, A & Wurst, W 2009, 'Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain', Development (Cambridge, England), vol. 136, no. 15, pp. 2545-2555. https://doi.org/10.1242/dev.031781

APA

Prakash, N., Puelles, E., Freude, K. K., Trümbach, D., Omodei, D., Di Salvio, M., Sussel, L., Ericson, J., Sander, M., Simeone, A., & Wurst, W. (2009). Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain. Development (Cambridge, England), 136(15), 2545-2555. https://doi.org/10.1242/dev.031781

Vancouver

Prakash N, Puelles E, Freude KK, Trümbach D, Omodei D, Di Salvio M et al. Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain. Development (Cambridge, England). 2009;136(15):2545-2555. https://doi.org/10.1242/dev.031781

Author

Prakash, Nilima ; Puelles, Eduardo ; Freude, Karla Kristine ; Trümbach, Dietrich ; Omodei, Daniela ; Di Salvio, Michela ; Sussel, Lori ; Ericson, Johan ; Sander, Maike ; Simeone, Antonio ; Wurst, Wolfgang. / Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain. In: Development (Cambridge, England). 2009 ; Vol. 136, No. 15. pp. 2545-2555.

Bibtex

@article{2fdaada64bf946e0ba7163d932feb50f,
title = "Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain",
abstract = "Little is known about the cues controlling the generation of motoneuron populations in the mammalian ventral midbrain. We show that Otx2 provides the crucial anterior-posterior positional information for the generation of red nucleus neurons in the murine midbrain. Moreover, the homeodomain transcription factor Nkx6-1 controls the proper development of the red nucleus and of the oculomotor and trochlear nucleus neurons. Nkx6-1 is expressed in ventral midbrain progenitors and acts as a fate determinant of the Brn3a(+) (also known as Pou4f1) red nucleus neurons. These progenitors are partially dorsalized in the absence of Nkx6-1, and a fraction of their postmitotic offspring adopts an alternative cell fate, as revealed by the activation of Dbx1 and Otx2 in these cells. Nkx6-1 is also expressed in postmitotic Isl1(+) oculomotor and trochlear neurons. Similar to hindbrain visceral (branchio-) motoneurons, Nkx6-1 controls the proper migration and axon outgrowth of these neurons by regulating the expression of at least three axon guidance/neuronal migration molecules. Based on these findings, we provide additional evidence that the developmental mechanism of the oculomotor and trochlear neurons exhibits more similarity with that of special visceral motoneurons than with that controlling the generation of somatic motoneurons located in the murine caudal hindbrain and spinal cord.",
keywords = "Animals, Axons, Cell Lineage, Cell Movement, Gene Expression Regulation, Developmental, Homeodomain Proteins, Mice, Mitosis, Models, Biological, Motor Neurons, Neurogenesis, Oculomotor Nerve, Otx Transcription Factors, Red Nucleus, Stem Cells, Transcription Factor Brn-3A, Trochlear Nerve",
author = "Nilima Prakash and Eduardo Puelles and Freude, {Karla Kristine} and Dietrich Tr{\"u}mbach and Daniela Omodei and {Di Salvio}, Michela and Lori Sussel and Johan Ericson and Maike Sander and Antonio Simeone and Wolfgang Wurst",
year = "2009",
doi = "10.1242/dev.031781",
language = "English",
volume = "136",
pages = "2545--2555",
journal = "Development",
issn = "0950-1991",
publisher = "The Company of Biologists",
number = "15",

}

RIS

TY - JOUR

T1 - Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain

AU - Prakash, Nilima

AU - Puelles, Eduardo

AU - Freude, Karla Kristine

AU - Trümbach, Dietrich

AU - Omodei, Daniela

AU - Di Salvio, Michela

AU - Sussel, Lori

AU - Ericson, Johan

AU - Sander, Maike

AU - Simeone, Antonio

AU - Wurst, Wolfgang

PY - 2009

Y1 - 2009

N2 - Little is known about the cues controlling the generation of motoneuron populations in the mammalian ventral midbrain. We show that Otx2 provides the crucial anterior-posterior positional information for the generation of red nucleus neurons in the murine midbrain. Moreover, the homeodomain transcription factor Nkx6-1 controls the proper development of the red nucleus and of the oculomotor and trochlear nucleus neurons. Nkx6-1 is expressed in ventral midbrain progenitors and acts as a fate determinant of the Brn3a(+) (also known as Pou4f1) red nucleus neurons. These progenitors are partially dorsalized in the absence of Nkx6-1, and a fraction of their postmitotic offspring adopts an alternative cell fate, as revealed by the activation of Dbx1 and Otx2 in these cells. Nkx6-1 is also expressed in postmitotic Isl1(+) oculomotor and trochlear neurons. Similar to hindbrain visceral (branchio-) motoneurons, Nkx6-1 controls the proper migration and axon outgrowth of these neurons by regulating the expression of at least three axon guidance/neuronal migration molecules. Based on these findings, we provide additional evidence that the developmental mechanism of the oculomotor and trochlear neurons exhibits more similarity with that of special visceral motoneurons than with that controlling the generation of somatic motoneurons located in the murine caudal hindbrain and spinal cord.

AB - Little is known about the cues controlling the generation of motoneuron populations in the mammalian ventral midbrain. We show that Otx2 provides the crucial anterior-posterior positional information for the generation of red nucleus neurons in the murine midbrain. Moreover, the homeodomain transcription factor Nkx6-1 controls the proper development of the red nucleus and of the oculomotor and trochlear nucleus neurons. Nkx6-1 is expressed in ventral midbrain progenitors and acts as a fate determinant of the Brn3a(+) (also known as Pou4f1) red nucleus neurons. These progenitors are partially dorsalized in the absence of Nkx6-1, and a fraction of their postmitotic offspring adopts an alternative cell fate, as revealed by the activation of Dbx1 and Otx2 in these cells. Nkx6-1 is also expressed in postmitotic Isl1(+) oculomotor and trochlear neurons. Similar to hindbrain visceral (branchio-) motoneurons, Nkx6-1 controls the proper migration and axon outgrowth of these neurons by regulating the expression of at least three axon guidance/neuronal migration molecules. Based on these findings, we provide additional evidence that the developmental mechanism of the oculomotor and trochlear neurons exhibits more similarity with that of special visceral motoneurons than with that controlling the generation of somatic motoneurons located in the murine caudal hindbrain and spinal cord.

KW - Animals

KW - Axons

KW - Cell Lineage

KW - Cell Movement

KW - Gene Expression Regulation, Developmental

KW - Homeodomain Proteins

KW - Mice

KW - Mitosis

KW - Models, Biological

KW - Motor Neurons

KW - Neurogenesis

KW - Oculomotor Nerve

KW - Otx Transcription Factors

KW - Red Nucleus

KW - Stem Cells

KW - Transcription Factor Brn-3A

KW - Trochlear Nerve

U2 - 10.1242/dev.031781

DO - 10.1242/dev.031781

M3 - Journal article

C2 - 19592574

VL - 136

SP - 2545

EP - 2555

JO - Development

JF - Development

SN - 0950-1991

IS - 15

ER -

ID: 138433875