Optimization of three-dimensional imaging on in vitro produced porcine blastocysts and chimeras for stem cell testing: a technology report.
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Optimization of three-dimensional imaging on in vitro produced porcine blastocysts and chimeras for stem cell testing : a technology report. / Secher, Jan Ole Bertelsen; Freude, Kristine; Li, Rong; Callesen, Henrik.
In: Stem Cells and Development, Vol. 24, No. 9, 2015, p. 1141-1145.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Optimization of three-dimensional imaging on in vitro produced porcine blastocysts and chimeras for stem cell testing
T2 - a technology report.
AU - Secher, Jan Ole Bertelsen
AU - Freude, Kristine
AU - Li, Rong
AU - Callesen, Henrik
PY - 2015
Y1 - 2015
N2 - Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testing of presumed pluripotent stem cells. The gold standard for pluripotent stem cells is to test if the cells are capable of contributing to germline chimeras. Differential staining can be used to evaluate the possibility of chimeric contribution; if the cells are located in the area of the ICM they are likely to contribute to the fetus and if they are located in the area of the TE they are likely to contribute to the fetal membranes. In this article, we optimize on methods for embryo staining and mounting so that the exact location of injected stem cells within preimplantation porcine embryos can be evaluated.
AB - Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testing of presumed pluripotent stem cells. The gold standard for pluripotent stem cells is to test if the cells are capable of contributing to germline chimeras. Differential staining can be used to evaluate the possibility of chimeric contribution; if the cells are located in the area of the ICM they are likely to contribute to the fetus and if they are located in the area of the TE they are likely to contribute to the fetal membranes. In this article, we optimize on methods for embryo staining and mounting so that the exact location of injected stem cells within preimplantation porcine embryos can be evaluated.
U2 - 10.1089/scd.2014.0503
DO - 10.1089/scd.2014.0503
M3 - Journal article
C2 - 25567670
VL - 24
SP - 1141
EP - 1145
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 9
ER -
ID: 138433367