Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish : Digestion method and viability studies. / Duflot, Maureen; Midelet, Graziella; Bourgau, Odile; Buchmann, Kurt; Gay, Mélanie.

In: Journal of Fish Diseases, Vol. 44, No. 11, 2021, p. 1777-1784.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Duflot, M, Midelet, G, Bourgau, O, Buchmann, K & Gay, M 2021, 'Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies', Journal of Fish Diseases, vol. 44, no. 11, pp. 1777-1784. https://doi.org/10.1111/jfd.13495

APA

Duflot, M., Midelet, G., Bourgau, O., Buchmann, K., & Gay, M. (2021). Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies. Journal of Fish Diseases, 44(11), 1777-1784. https://doi.org/10.1111/jfd.13495

Vancouver

Duflot M, Midelet G, Bourgau O, Buchmann K, Gay M. Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies. Journal of Fish Diseases. 2021;44(11):1777-1784. https://doi.org/10.1111/jfd.13495

Author

Duflot, Maureen ; Midelet, Graziella ; Bourgau, Odile ; Buchmann, Kurt ; Gay, Mélanie. / Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish : Digestion method and viability studies. In: Journal of Fish Diseases. 2021 ; Vol. 44, No. 11. pp. 1777-1784.

Bibtex

@article{187cd7a8da4745ac9ade677ec8e8aa11,
title = "Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies",
abstract = "Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of Cryptocotyle metacercariae in fish post-mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of Cryptocotyle infection. Moreover, morphological identification seemed reliable up to 8 days pm for Cryptocotyle infection.",
author = "Maureen Duflot and Graziella Midelet and Odile Bourgau and Kurt Buchmann and M{\'e}lanie Gay",
year = "2021",
doi = "10.1111/jfd.13495",
language = "English",
volume = "44",
pages = "1777--1784",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
number = "11",

}

RIS

TY - JOUR

T1 - Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish

T2 - Digestion method and viability studies

AU - Duflot, Maureen

AU - Midelet, Graziella

AU - Bourgau, Odile

AU - Buchmann, Kurt

AU - Gay, Mélanie

PY - 2021

Y1 - 2021

N2 - Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of Cryptocotyle metacercariae in fish post-mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of Cryptocotyle infection. Moreover, morphological identification seemed reliable up to 8 days pm for Cryptocotyle infection.

AB - Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of Cryptocotyle metacercariae in fish post-mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of Cryptocotyle infection. Moreover, morphological identification seemed reliable up to 8 days pm for Cryptocotyle infection.

U2 - 10.1111/jfd.13495

DO - 10.1111/jfd.13495

M3 - Journal article

C2 - 34289140

VL - 44

SP - 1777

EP - 1784

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 11

ER -

ID: 275015758