Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers. / Klærke, Dan Arne; Tejada, Maria de los Angeles; Christensen, Vibeke Grøsfjeld; Lassen, Mette; Pedersen, Per Amstrup; Callø, Kirstine.

In: Current Protocols in Pharmacology, Vol. 81, No. 1, e37, 2018, p. e37.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klærke, DA, Tejada, MDLA, Christensen, VG, Lassen, M, Pedersen, PA & Callø, K 2018, 'Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers', Current Protocols in Pharmacology, vol. 81, no. 1, e37, pp. e37. https://doi.org/10.1002/cpph.37

APA

Klærke, D. A., Tejada, M. D. L. A., Christensen, V. G., Lassen, M., Pedersen, P. A., & Callø, K. (2018). Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers. Current Protocols in Pharmacology, 81(1), e37. [e37]. https://doi.org/10.1002/cpph.37

Vancouver

Klærke DA, Tejada MDLA, Christensen VG, Lassen M, Pedersen PA, Callø K. Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers. Current Protocols in Pharmacology. 2018;81(1):e37. e37. https://doi.org/10.1002/cpph.37

Author

Klærke, Dan Arne ; Tejada, Maria de los Angeles ; Christensen, Vibeke Grøsfjeld ; Lassen, Mette ; Pedersen, Per Amstrup ; Callø, Kirstine. / Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers. In: Current Protocols in Pharmacology. 2018 ; Vol. 81, No. 1. pp. e37.

Bibtex

@article{3d42e858757845639e249b407f1ac51d,
title = "Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers",
abstract = "Detergent‐solubilized purified ion channels can be reconstituted into lipid bilayers for electrophysiological analysis. Traditionally, ion channels were inserted into vesicles and subsequently fused with planar “black lipid membranes” formed from lipids dissolved in a hydrophobic solvent such as decane. Provided in this article is a step‐by‐step guide to reconstitute purified ion channel proteins into giant unilamellar vesicles (GUVs). This procedure results in the formation of proteoliposomes that can be used for planar bilayer formation and electrophysiological characterization of single‐channel currents. By using preformed GUVs it is possible to omit the membrane solvent. Compared to traditional preparations, the lipid bilayers formed from GUVs provide an environment that more closely resembles the native cell membrane. Also described is an alternate protocol that entails the production of planar lipid bilayers from GUVs onto which proteins in detergent are added. ",
author = "Kl{\ae}rke, {Dan Arne} and Tejada, {Maria de los Angeles} and Christensen, {Vibeke Gr{\o}sfjeld} and Mette Lassen and Pedersen, {Per Amstrup} and Kirstine Call{\o}",
note = "Copyright {\textcopyright} 2018 John Wiley & Sons, Inc.",
year = "2018",
doi = "10.1002/cpph.37",
language = "English",
volume = "81",
pages = "e37",
journal = "Current Protocols in Pharmacology",
issn = "1934-8282",
publisher = "JohnWiley & Sons, Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers

AU - Klærke, Dan Arne

AU - Tejada, Maria de los Angeles

AU - Christensen, Vibeke Grøsfjeld

AU - Lassen, Mette

AU - Pedersen, Per Amstrup

AU - Callø, Kirstine

N1 - Copyright © 2018 John Wiley & Sons, Inc.

PY - 2018

Y1 - 2018

N2 - Detergent‐solubilized purified ion channels can be reconstituted into lipid bilayers for electrophysiological analysis. Traditionally, ion channels were inserted into vesicles and subsequently fused with planar “black lipid membranes” formed from lipids dissolved in a hydrophobic solvent such as decane. Provided in this article is a step‐by‐step guide to reconstitute purified ion channel proteins into giant unilamellar vesicles (GUVs). This procedure results in the formation of proteoliposomes that can be used for planar bilayer formation and electrophysiological characterization of single‐channel currents. By using preformed GUVs it is possible to omit the membrane solvent. Compared to traditional preparations, the lipid bilayers formed from GUVs provide an environment that more closely resembles the native cell membrane. Also described is an alternate protocol that entails the production of planar lipid bilayers from GUVs onto which proteins in detergent are added.

AB - Detergent‐solubilized purified ion channels can be reconstituted into lipid bilayers for electrophysiological analysis. Traditionally, ion channels were inserted into vesicles and subsequently fused with planar “black lipid membranes” formed from lipids dissolved in a hydrophobic solvent such as decane. Provided in this article is a step‐by‐step guide to reconstitute purified ion channel proteins into giant unilamellar vesicles (GUVs). This procedure results in the formation of proteoliposomes that can be used for planar bilayer formation and electrophysiological characterization of single‐channel currents. By using preformed GUVs it is possible to omit the membrane solvent. Compared to traditional preparations, the lipid bilayers formed from GUVs provide an environment that more closely resembles the native cell membrane. Also described is an alternate protocol that entails the production of planar lipid bilayers from GUVs onto which proteins in detergent are added.

U2 - 10.1002/cpph.37

DO - 10.1002/cpph.37

M3 - Journal article

C2 - 29927074

VL - 81

SP - e37

JO - Current Protocols in Pharmacology

JF - Current Protocols in Pharmacology

SN - 1934-8282

IS - 1

M1 - e37

ER -

ID: 198565556