RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols. / Vegni, Jacopo; Sun, Ying; Seemann, Stefan E.; Zappaterra, Martina; Davoli, Roberta; Dall’Olio, Stefania; Gorodkin, Jan; Zambonelli, Paolo.

In: Animals, Vol. 13, No. 7, 1187, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vegni, J, Sun, Y, Seemann, SE, Zappaterra, M, Davoli, R, Dall’Olio, S, Gorodkin, J & Zambonelli, P 2023, 'RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols', Animals, vol. 13, no. 7, 1187. https://doi.org/10.3390/ani13071187

APA

Vegni, J., Sun, Y., Seemann, S. E., Zappaterra, M., Davoli, R., Dall’Olio, S., Gorodkin, J., & Zambonelli, P. (2023). RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols. Animals, 13(7), [1187]. https://doi.org/10.3390/ani13071187

Vancouver

Vegni J, Sun Y, Seemann SE, Zappaterra M, Davoli R, Dall’Olio S et al. RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols. Animals. 2023;13(7). 1187. https://doi.org/10.3390/ani13071187

Author

Vegni, Jacopo ; Sun, Ying ; Seemann, Stefan E. ; Zappaterra, Martina ; Davoli, Roberta ; Dall’Olio, Stefania ; Gorodkin, Jan ; Zambonelli, Paolo. / RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols. In: Animals. 2023 ; Vol. 13, No. 7.

Bibtex

@article{bbda693949e74a3387666580bb932ae7,
title = "RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols",
abstract = "The addition of n-3 polyunsaturated fatty acids (n-3 PUFAs) to the swine diet increases their content in muscle cells, and the additional supplementation of antioxidants promotes their oxidative stability. However, to date, the functionality of these components within muscle tissue is not well understood. Using a published RNA-seq dataset and a selective workflow, the study aimed to find the differences in gene expression and investigate how differentially expressed genes (DEGs) were implicated in the cellular composition and metabolism of muscle tissue of 48 Italian Large White pigs under different dietary conditions. A functional enrichment analysis of DEGs, using Cytoscape, revealed that the diet enriched with extruded linseed and supplemented with vitamin E and selenium promoted a more rapid and massive immune system response because the overall function of muscle tissue was improved, while those enriched with extruded linseed and supplemented with grape skin and oregano extracts promoted the presence and oxidative stability of n-3 PUFAs, increasing the anti-inflammatory potential of the muscular tissue.",
keywords = "antioxidant, methodological approach for RNA-seq data analysis, polyphenols, polyunsaturated fatty acid (PUFA), selenium, swine, transcriptome, vitamin E",
author = "Jacopo Vegni and Ying Sun and Seemann, {Stefan E.} and Martina Zappaterra and Roberta Davoli and Stefania Dall{\textquoteright}Olio and Jan Gorodkin and Paolo Zambonelli",
note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",
year = "2023",
doi = "10.3390/ani13071187",
language = "English",
volume = "13",
journal = "Animals",
issn = "2076-2615",
publisher = "MDPI",
number = "7",

}

RIS

TY - JOUR

T1 - RNA-Seq Study on the Longissimus thoracis Muscle of Italian Large White Pigs Fed Extruded Linseed with or without Antioxidants and Polyphenols

AU - Vegni, Jacopo

AU - Sun, Ying

AU - Seemann, Stefan E.

AU - Zappaterra, Martina

AU - Davoli, Roberta

AU - Dall’Olio, Stefania

AU - Gorodkin, Jan

AU - Zambonelli, Paolo

N1 - Publisher Copyright: © 2023 by the authors.

PY - 2023

Y1 - 2023

N2 - The addition of n-3 polyunsaturated fatty acids (n-3 PUFAs) to the swine diet increases their content in muscle cells, and the additional supplementation of antioxidants promotes their oxidative stability. However, to date, the functionality of these components within muscle tissue is not well understood. Using a published RNA-seq dataset and a selective workflow, the study aimed to find the differences in gene expression and investigate how differentially expressed genes (DEGs) were implicated in the cellular composition and metabolism of muscle tissue of 48 Italian Large White pigs under different dietary conditions. A functional enrichment analysis of DEGs, using Cytoscape, revealed that the diet enriched with extruded linseed and supplemented with vitamin E and selenium promoted a more rapid and massive immune system response because the overall function of muscle tissue was improved, while those enriched with extruded linseed and supplemented with grape skin and oregano extracts promoted the presence and oxidative stability of n-3 PUFAs, increasing the anti-inflammatory potential of the muscular tissue.

AB - The addition of n-3 polyunsaturated fatty acids (n-3 PUFAs) to the swine diet increases their content in muscle cells, and the additional supplementation of antioxidants promotes their oxidative stability. However, to date, the functionality of these components within muscle tissue is not well understood. Using a published RNA-seq dataset and a selective workflow, the study aimed to find the differences in gene expression and investigate how differentially expressed genes (DEGs) were implicated in the cellular composition and metabolism of muscle tissue of 48 Italian Large White pigs under different dietary conditions. A functional enrichment analysis of DEGs, using Cytoscape, revealed that the diet enriched with extruded linseed and supplemented with vitamin E and selenium promoted a more rapid and massive immune system response because the overall function of muscle tissue was improved, while those enriched with extruded linseed and supplemented with grape skin and oregano extracts promoted the presence and oxidative stability of n-3 PUFAs, increasing the anti-inflammatory potential of the muscular tissue.

KW - antioxidant

KW - methodological approach for RNA-seq data analysis

KW - polyphenols

KW - polyunsaturated fatty acid (PUFA)

KW - selenium

KW - swine

KW - transcriptome

KW - vitamin E

U2 - 10.3390/ani13071187

DO - 10.3390/ani13071187

M3 - Journal article

C2 - 37048443

AN - SCOPUS:85152684246

VL - 13

JO - Animals

JF - Animals

SN - 2076-2615

IS - 7

M1 - 1187

ER -

ID: 345423189