SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization

Research output: Contribution to journal › Journal article › Research › peer-review

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SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization. / Damas, Nkerorema Djodji; Marcatti, Michela; Côme, Christophe; Christensen, Lise-Lotte; Nielsen, Morten Muhlig; Baumgartner, Roland; Gylling, Helene Maria; Maglieri, Giulia; Rundsten, Carsten Friis; Seemann, Ernst Stefan; Rapin, Nicolas; Thézenas, Simon; Vang, Søren; Ørntoft, Torben; Andersen, Claus Lindbjerg; Pedersen, Jakob Skou; Lund, Anders H.

In: Nature Communications, Vol. 7, 13875, 22.12.2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Damas, ND, Marcatti, M, Côme, C, Christensen, L-L, Nielsen, MM, Baumgartner, R, Gylling, HM, Maglieri, G, Rundsten, CF, Seemann, ES, Rapin, N, Thézenas, S, Vang, S, Ørntoft, T, Andersen, CL, Pedersen, JS & Lund, AH 2016, 'SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization', Nature Communications, vol. 7, 13875. https://doi.org/10.1038/ncomms13875

APA

Damas, N. D., Marcatti, M., Côme, C., Christensen, L-L., Nielsen, M. M., Baumgartner, R., Gylling, H. M., Maglieri, G., Rundsten, C. F., Seemann, E. S., Rapin, N., Thézenas, S., Vang, S., Ørntoft, T., Andersen, C. L., Pedersen, J. S., & Lund, A. H. (2016). SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization. Nature Communications, 7, [13875]. https://doi.org/10.1038/ncomms13875

Vancouver

Damas ND, Marcatti M, Côme C, Christensen L-L, Nielsen MM, Baumgartner R et al. SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization. Nature Communications. 2016 Dec 22;7. 13875. https://doi.org/10.1038/ncomms13875

Author

Damas, Nkerorema Djodji ; Marcatti, Michela ; Côme, Christophe ; Christensen, Lise-Lotte ; Nielsen, Morten Muhlig ; Baumgartner, Roland ; Gylling, Helene Maria ; Maglieri, Giulia ; Rundsten, Carsten Friis ; Seemann, Ernst Stefan ; Rapin, Nicolas ; Thézenas, Simon ; Vang, Søren ; Ørntoft, Torben ; Andersen, Claus Lindbjerg ; Pedersen, Jakob Skou ; Lund, Anders H. / SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization. In: Nature Communications. 2016 ; Vol. 7.

Bibtex

@article{634c590db4f8474a81077243fdddf0f7,
title = "SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization",
abstract = "We currently have limited knowledge of the involvement of long non-coding RNAs (lncRNAs) in normal cellular processes and pathologies. Here, we identify and characterize SNHG5 as a stable cytoplasmic lncRNA with up-regulated expression in colorectal cancer. Depletion of SNHG5 induces cell cycle arrest and apoptosis in vitro and limits tumour outgrowth in vivo, whereas SNHG5 overexpression counteracts oxaliplatin-induced apoptosis. Using an unbiased approach, we identify 121 transcript sites interacting with SNHG5 in the cytoplasm. Importantly, knockdown of key SNHG5 target transcripts, including SPATS2, induces apoptosis and thus mimics the effect seen following SNHG5 depletion. Mechanistically, we suggest that SNHG5 stabilizes the target transcripts by blocking their degradation by STAU1. Accordingly, depletion of STAU1 rescues the apoptosis induced after SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting tumour cell survival in colorectal cancer and delineate a novel mechanism in which a cytoplasmic lncRNA functions through blocking the action of STAU1.",
author = "Damas, {Nkerorema Djodji} and Michela Marcatti and Christophe C{\^o}me and Lise-Lotte Christensen and Nielsen, {Morten Muhlig} and Roland Baumgartner and Gylling, {Helene Maria} and Giulia Maglieri and Rundsten, {Carsten Friis} and Seemann, {Ernst Stefan} and Nicolas Rapin and Simon Th{\'e}zenas and S{\o}ren Vang and Torben {\O}rntoft and Andersen, {Claus Lindbjerg} and Pedersen, {Jakob Skou} and Lund, {Anders H}",
year = "2016",
month = dec,
day = "22",
doi = "10.1038/ncomms13875",
language = "English",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization

AU - Damas, Nkerorema Djodji

AU - Marcatti, Michela

AU - Côme, Christophe

AU - Christensen, Lise-Lotte

AU - Nielsen, Morten Muhlig

AU - Baumgartner, Roland

AU - Gylling, Helene Maria

AU - Maglieri, Giulia

AU - Rundsten, Carsten Friis

AU - Seemann, Ernst Stefan

AU - Rapin, Nicolas

AU - Thézenas, Simon

AU - Vang, Søren

AU - Ørntoft, Torben

AU - Andersen, Claus Lindbjerg

AU - Pedersen, Jakob Skou

AU - Lund, Anders H

PY - 2016/12/22

Y1 - 2016/12/22

N2 - We currently have limited knowledge of the involvement of long non-coding RNAs (lncRNAs) in normal cellular processes and pathologies. Here, we identify and characterize SNHG5 as a stable cytoplasmic lncRNA with up-regulated expression in colorectal cancer. Depletion of SNHG5 induces cell cycle arrest and apoptosis in vitro and limits tumour outgrowth in vivo, whereas SNHG5 overexpression counteracts oxaliplatin-induced apoptosis. Using an unbiased approach, we identify 121 transcript sites interacting with SNHG5 in the cytoplasm. Importantly, knockdown of key SNHG5 target transcripts, including SPATS2, induces apoptosis and thus mimics the effect seen following SNHG5 depletion. Mechanistically, we suggest that SNHG5 stabilizes the target transcripts by blocking their degradation by STAU1. Accordingly, depletion of STAU1 rescues the apoptosis induced after SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting tumour cell survival in colorectal cancer and delineate a novel mechanism in which a cytoplasmic lncRNA functions through blocking the action of STAU1.

AB - We currently have limited knowledge of the involvement of long non-coding RNAs (lncRNAs) in normal cellular processes and pathologies. Here, we identify and characterize SNHG5 as a stable cytoplasmic lncRNA with up-regulated expression in colorectal cancer. Depletion of SNHG5 induces cell cycle arrest and apoptosis in vitro and limits tumour outgrowth in vivo, whereas SNHG5 overexpression counteracts oxaliplatin-induced apoptosis. Using an unbiased approach, we identify 121 transcript sites interacting with SNHG5 in the cytoplasm. Importantly, knockdown of key SNHG5 target transcripts, including SPATS2, induces apoptosis and thus mimics the effect seen following SNHG5 depletion. Mechanistically, we suggest that SNHG5 stabilizes the target transcripts by blocking their degradation by STAU1. Accordingly, depletion of STAU1 rescues the apoptosis induced after SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting tumour cell survival in colorectal cancer and delineate a novel mechanism in which a cytoplasmic lncRNA functions through blocking the action of STAU1.

U2 - 10.1038/ncomms13875

DO - 10.1038/ncomms13875

M3 - Journal article

C2 - 28004750

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 13875

ER -

ID: 170364201