Structural profiles of human miRNA families from pairwise clustering
Research output: Contribution to journal › Journal article › Research › peer-review
MicroRNAs (miRNAs) are a group of small, approximately 21 nt long, riboregulators inhibiting gene expression at a post-transcriptional level. Their most distinctive structural feature is the foldback hairpin of their precursor pre-miRNAs. Even though each pre-miRNA deposited in miRBase has its secondary structure already predicted, little is known about the patterns of structural conservation among pre-miRNAs. We address this issue by clustering the human pre-miRNA sequences based on pairwise, sequence and secondary structure alignment using FOLDALIGN, followed by global multiple alignment of obtained clusters by WAR. As a result, the common secondary structure was successfully determined for four FOLDALIGN clusters: the RF00027 structural family of the Rfam database and three clusters with previously undescribed consensus structures. Availability: http://genome.ku.dk/resources/mirclust
| Original language | English |
|---|---|
| Journal | Bioinformatics |
| Volume | 25 |
| Issue number | 3 |
| Pages (from-to) | 291-294 |
| Number of pages | 4 |
| ISSN | 1367-4803 |
| DOIs | |
| Publication status | Published - 2009 |
ID: 18203385