The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF. / Zhou, Shuling; Ochalek, Anna; Szczesna, Karolina; Avci, Hasan X.; Kobolak, Julianna; Varga, Eszter; Rasmussen, Mikkei; Holst, Bjørn; Cirera Salicio, Susanna; Hyttel, Poul; Freude, Kristine K.; Dinnyes, Andras.

In: Differentiation, Vol. 92, No. 4, 10.2016, p. 183-194.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhou, S, Ochalek, A, Szczesna, K, Avci, HX, Kobolak, J, Varga, E, Rasmussen, M, Holst, B, Cirera Salicio, S, Hyttel, P, Freude, KK & Dinnyes, A 2016, 'The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF', Differentiation, vol. 92, no. 4, pp. 183-194. https://doi.org/10.1016/j.diff.2016.06.002

APA

Zhou, S., Ochalek, A., Szczesna, K., Avci, H. X., Kobolak, J., Varga, E., Rasmussen, M., Holst, B., Cirera Salicio, S., Hyttel, P., Freude, K. K., & Dinnyes, A. (2016). The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF. Differentiation, 92(4), 183-194. https://doi.org/10.1016/j.diff.2016.06.002

Vancouver

Zhou S, Ochalek A, Szczesna K, Avci HX, Kobolak J, Varga E et al. The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF. Differentiation. 2016 Oct;92(4):183-194. https://doi.org/10.1016/j.diff.2016.06.002

Author

Zhou, Shuling ; Ochalek, Anna ; Szczesna, Karolina ; Avci, Hasan X. ; Kobolak, Julianna ; Varga, Eszter ; Rasmussen, Mikkei ; Holst, Bjørn ; Cirera Salicio, Susanna ; Hyttel, Poul ; Freude, Kristine K. ; Dinnyes, Andras. / The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF. In: Differentiation. 2016 ; Vol. 92, No. 4. pp. 183-194.

Bibtex

@article{519edca66c82441d8b5f5a0a5a7ecf54,
title = "The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF",
abstract = "Neural rosettes derived from human induced pluripotent stem cells (iPSCs) have been claimed to be a highly robust in vitro cellular model for biomedical application. They are able to propagate in vitro in the presence of mitogens, including basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). However, these two mitogens are also involved in anterior-posterior patterning in a gradient dependent manner along the neural tube axis.Here, we compared the regional identity of neural rosette cells and specific neural subtypes of their progeny propagated with low and high concentrations of bFGF and EGF. We observed that low concentrations of bFGF and EGF in the culturing system were able to induce forebrain identity of the neural rosettes and promote subsequent cortical neuronal differentiation. On the contrary, high concentrations of these mitogens stimulate a mid-hindbrain fate of the neural rosettes, resulting in subsequent cholinergic neuron differentiation. Thus, our results indicate that different concentrations of bFGF and EGF supplemented during propagation of neural rosettes are involved in altering the identity of the resultant neural cells.",
keywords = "Mitogens, Neural rosette, Forebrain NPCs, Mid-hindbrain NPCs, Glutamatergic neurons, Cholinergic neurons",
author = "Shuling Zhou and Anna Ochalek and Karolina Szczesna and Avci, {Hasan X.} and Julianna Kobolak and Eszter Varga and Mikkei Rasmussen and Bj{\o}rn Holst and {Cirera Salicio}, Susanna and Poul Hyttel and Freude, {Kristine K.} and Andras Dinnyes",
year = "2016",
month = oct,
doi = "10.1016/j.diff.2016.06.002",
language = "English",
volume = "92",
pages = "183--194",
journal = "Differentiation",
issn = "0301-4681",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF

AU - Zhou, Shuling

AU - Ochalek, Anna

AU - Szczesna, Karolina

AU - Avci, Hasan X.

AU - Kobolak, Julianna

AU - Varga, Eszter

AU - Rasmussen, Mikkei

AU - Holst, Bjørn

AU - Cirera Salicio, Susanna

AU - Hyttel, Poul

AU - Freude, Kristine K.

AU - Dinnyes, Andras

PY - 2016/10

Y1 - 2016/10

N2 - Neural rosettes derived from human induced pluripotent stem cells (iPSCs) have been claimed to be a highly robust in vitro cellular model for biomedical application. They are able to propagate in vitro in the presence of mitogens, including basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). However, these two mitogens are also involved in anterior-posterior patterning in a gradient dependent manner along the neural tube axis.Here, we compared the regional identity of neural rosette cells and specific neural subtypes of their progeny propagated with low and high concentrations of bFGF and EGF. We observed that low concentrations of bFGF and EGF in the culturing system were able to induce forebrain identity of the neural rosettes and promote subsequent cortical neuronal differentiation. On the contrary, high concentrations of these mitogens stimulate a mid-hindbrain fate of the neural rosettes, resulting in subsequent cholinergic neuron differentiation. Thus, our results indicate that different concentrations of bFGF and EGF supplemented during propagation of neural rosettes are involved in altering the identity of the resultant neural cells.

AB - Neural rosettes derived from human induced pluripotent stem cells (iPSCs) have been claimed to be a highly robust in vitro cellular model for biomedical application. They are able to propagate in vitro in the presence of mitogens, including basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). However, these two mitogens are also involved in anterior-posterior patterning in a gradient dependent manner along the neural tube axis.Here, we compared the regional identity of neural rosette cells and specific neural subtypes of their progeny propagated with low and high concentrations of bFGF and EGF. We observed that low concentrations of bFGF and EGF in the culturing system were able to induce forebrain identity of the neural rosettes and promote subsequent cortical neuronal differentiation. On the contrary, high concentrations of these mitogens stimulate a mid-hindbrain fate of the neural rosettes, resulting in subsequent cholinergic neuron differentiation. Thus, our results indicate that different concentrations of bFGF and EGF supplemented during propagation of neural rosettes are involved in altering the identity of the resultant neural cells.

KW - Mitogens

KW - Neural rosette

KW - Forebrain NPCs

KW - Mid-hindbrain NPCs

KW - Glutamatergic neurons

KW - Cholinergic neurons

U2 - 10.1016/j.diff.2016.06.002

DO - 10.1016/j.diff.2016.06.002

M3 - Journal article

C2 - 27321088

VL - 92

SP - 183

EP - 194

JO - Differentiation

JF - Differentiation

SN - 0301-4681

IS - 4

ER -

ID: 169761351