Breeding schemes for intervertebral disc disease in dachshunds: Is disc calcification score preferable to genotyping of the FGF4 retrogene insertion on CFA12?
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Breeding schemes for intervertebral disc disease in dachshunds : Is disc calcification score preferable to genotyping of the FGF4 retrogene insertion on CFA12? / Bruun, Camilla Sichlau; Bruun, Charlotte; Marx, Tine; Proschowsky, Helle Friis; Fredholm, Merete.
In: Canine medicine and genetics, Vol. 7, 18, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Breeding schemes for intervertebral disc disease in dachshunds
T2 - Is disc calcification score preferable to genotyping of the FGF4 retrogene insertion on CFA12?
AU - Bruun, Camilla Sichlau
AU - Bruun, Charlotte
AU - Marx, Tine
AU - Proschowsky, Helle Friis
AU - Fredholm, Merete
PY - 2020
Y1 - 2020
N2 - BACKGROUND: Approximately every fifth Dachshund is affected by disc herniation - a painful, hereditary condition which is typically preceded by disc calcification. Therefore, the selection of dogs suitable for breeding can be based on radiographic examination of calcification status. Recently, an insertion of an FGF4 retrogene on CFA12 has been identified and associated with the risk of developing disc herniation in chondrodystrophic breeds and a DNA test is now offered. In this study we investigate the incidence of disc herniation in the smooth-haired, long-haired and wire- haired Dachshund populations. We also evaluate and compare the accuracy of the two breeding schemes predicting the risk of disc herniation: the DNA test and the radiography based scheme.RESULTS: The overall incidence of disc herniation in Danish Dachshunds was 18% and no significant difference was found between the long-haired (17%), smooth-haired (22%) and wire-haired (16%) populations (p > 0.05). We found a significant association (p < 0.0001) between calcification status and the risk of disc herniation with a relative risk of 14.78. Using calcification status (≥ 5 or < 5 calcifications) as a risk indicator has a sensitivity of 0.79 and a specificity of 0.91. A significant association between the FGF4 retrogene insertion and the disc calcification status was found in the wire-haired population (p < 0.0001) where the DNA test has a sensitivity of 1.0 and a specificity of 0.14. In the long- and smooth-haired populations no association was found (p > 0.05) and here the insertion allele was almost fixed.CONCLUSION: Our results show that the FGF4 retrogene insertion on CFA12 is not a valid risk indicator on its own. Relying on the DNA test will have an irreversible effect on the Dachshund breed excluding almost all dogs from breeding. Thus, using calcification status remains the most reliable breeding scheme for disc herniation in Dachshunds.
AB - BACKGROUND: Approximately every fifth Dachshund is affected by disc herniation - a painful, hereditary condition which is typically preceded by disc calcification. Therefore, the selection of dogs suitable for breeding can be based on radiographic examination of calcification status. Recently, an insertion of an FGF4 retrogene on CFA12 has been identified and associated with the risk of developing disc herniation in chondrodystrophic breeds and a DNA test is now offered. In this study we investigate the incidence of disc herniation in the smooth-haired, long-haired and wire- haired Dachshund populations. We also evaluate and compare the accuracy of the two breeding schemes predicting the risk of disc herniation: the DNA test and the radiography based scheme.RESULTS: The overall incidence of disc herniation in Danish Dachshunds was 18% and no significant difference was found between the long-haired (17%), smooth-haired (22%) and wire-haired (16%) populations (p > 0.05). We found a significant association (p < 0.0001) between calcification status and the risk of disc herniation with a relative risk of 14.78. Using calcification status (≥ 5 or < 5 calcifications) as a risk indicator has a sensitivity of 0.79 and a specificity of 0.91. A significant association between the FGF4 retrogene insertion and the disc calcification status was found in the wire-haired population (p < 0.0001) where the DNA test has a sensitivity of 1.0 and a specificity of 0.14. In the long- and smooth-haired populations no association was found (p > 0.05) and here the insertion allele was almost fixed.CONCLUSION: Our results show that the FGF4 retrogene insertion on CFA12 is not a valid risk indicator on its own. Relying on the DNA test will have an irreversible effect on the Dachshund breed excluding almost all dogs from breeding. Thus, using calcification status remains the most reliable breeding scheme for disc herniation in Dachshunds.
U2 - 10.1186/s40575-020-00096-6
DO - 10.1186/s40575-020-00096-6
M3 - Journal article
C2 - 33292664
VL - 7
JO - Canine medicine and genetics
JF - Canine medicine and genetics
SN - 2662-9380
M1 - 18
ER -
ID: 259673637