Protection against SARS-CoV-2 transmission by a parenteral prime—Intranasal boost vaccine strategy
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Protection against SARS-CoV-2 transmission by a parenteral prime—Intranasal boost vaccine strategy. / Christensen, Dennis; Polacek, Charlotta; Sheward, Daniel J.; Hanke, Leo; Moliner-morro, Ainhoa; Mcinerney, Gerald; Murrell, Ben; Hartmann, Katrine Top; Jensen, Henrik Elvang; Jungersen, Gregers; Illigen, Kristin; Isling, Louise Krag; Jensen, Rune Fledelius; Hansen, Julia Sid; Rosenkrands, Ida; Fernandez-antunez, Carlota; Ramirez, Santseharay; Follmann, Frank; Bukh, Jens; Pedersen, Gabriel Kristian.
In: EBioMedicine, Vol. 84, 104248, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Protection against SARS-CoV-2 transmission by a parenteral prime—Intranasal boost vaccine strategy
AU - Christensen, Dennis
AU - Polacek, Charlotta
AU - Sheward, Daniel J.
AU - Hanke, Leo
AU - Moliner-morro, Ainhoa
AU - Mcinerney, Gerald
AU - Murrell, Ben
AU - Hartmann, Katrine Top
AU - Jensen, Henrik Elvang
AU - Jungersen, Gregers
AU - Illigen, Kristin
AU - Isling, Louise Krag
AU - Jensen, Rune Fledelius
AU - Hansen, Julia Sid
AU - Rosenkrands, Ida
AU - Fernandez-antunez, Carlota
AU - Ramirez, Santseharay
AU - Follmann, Frank
AU - Bukh, Jens
AU - Pedersen, Gabriel Kristian
PY - 2022
Y1 - 2022
N2 - BackgroundLicensed vaccines against SARS-CoV-2 effectively protect against severe disease, but display incomplete protection against virus transmission. Mucosal vaccines providing immune responses in the upper airways are one strategy to protect against transmission.MethodsWe administered Spike HexaPro trimer formulated in a cationic liposomal adjuvant as a parenteral (subcutaneous – s.c.) prime - intranasal boost regimen to elicit airway mucosal immune responses and evaluated this in a Syrian hamster model of virus transmission.FindingsParenteral prime - intranasal boost elicited high-magnitude serum neutralizing antibody responses and IgA responses in the upper respiratory tract. The vaccine strategy protected against virus in the lower airways and lung pathology, but virus could still be detected in the upper airways. Despite this, the parenteral prime - intranasal booster vaccine effectively protected against onward SARS-CoV-2 transmission.InterpretationThis study suggests that parenteral-prime mucosal boost is an effective strategy for protecting against SARS-CoV-2 infection and highlights that protection against virus transmission may be obtained despite incomplete clearance of virus from the upper respiratory tract. It should be noted that protection against onward transmission was not compared to standard parenteral prime-boost, which should be a focus for future studies.FundingThis work was primarily supported by the European Union Horizon 2020 research and innovation program under grant agreement no. 101003653.
AB - BackgroundLicensed vaccines against SARS-CoV-2 effectively protect against severe disease, but display incomplete protection against virus transmission. Mucosal vaccines providing immune responses in the upper airways are one strategy to protect against transmission.MethodsWe administered Spike HexaPro trimer formulated in a cationic liposomal adjuvant as a parenteral (subcutaneous – s.c.) prime - intranasal boost regimen to elicit airway mucosal immune responses and evaluated this in a Syrian hamster model of virus transmission.FindingsParenteral prime - intranasal boost elicited high-magnitude serum neutralizing antibody responses and IgA responses in the upper respiratory tract. The vaccine strategy protected against virus in the lower airways and lung pathology, but virus could still be detected in the upper airways. Despite this, the parenteral prime - intranasal booster vaccine effectively protected against onward SARS-CoV-2 transmission.InterpretationThis study suggests that parenteral-prime mucosal boost is an effective strategy for protecting against SARS-CoV-2 infection and highlights that protection against virus transmission may be obtained despite incomplete clearance of virus from the upper respiratory tract. It should be noted that protection against onward transmission was not compared to standard parenteral prime-boost, which should be a focus for future studies.FundingThis work was primarily supported by the European Union Horizon 2020 research and innovation program under grant agreement no. 101003653.
U2 - 10.1016/j.ebiom.2022.104248
DO - 10.1016/j.ebiom.2022.104248
M3 - Journal article
C2 - 36088218
VL - 84
JO - EBioMedicine
JF - EBioMedicine
SN - 2352-3964
M1 - 104248
ER -
ID: 319241143