Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations

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Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations. / Kristensen, Charlotte; Laybourn, Helena A.; Crumpton, Jeri Carol; Martiny, Karen; Webb, Ashley; Ryt-Hansen, Pia; Trebbien, Ramona; Jensen, Henrik E.; Nissen, Jakob N.; Skovgaard, Kerstin; Webby, Richard J.; Larsen, Lars E.

In: PLoS Pathogens, Vol. 19, No. 12, e1011838, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kristensen, C, Laybourn, HA, Crumpton, JC, Martiny, K, Webb, A, Ryt-Hansen, P, Trebbien, R, Jensen, HE, Nissen, JN, Skovgaard, K, Webby, RJ & Larsen, LE 2023, 'Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations', PLoS Pathogens, vol. 19, no. 12, e1011838. https://doi.org/10.1371/journal.ppat.1011838

APA

Kristensen, C., Laybourn, H. A., Crumpton, J. C., Martiny, K., Webb, A., Ryt-Hansen, P., Trebbien, R., Jensen, H. E., Nissen, J. N., Skovgaard, K., Webby, R. J., & Larsen, L. E. (2023). Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations. PLoS Pathogens, 19(12), [e1011838]. https://doi.org/10.1371/journal.ppat.1011838

Vancouver

Kristensen C, Laybourn HA, Crumpton JC, Martiny K, Webb A, Ryt-Hansen P et al. Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations. PLoS Pathogens. 2023;19(12). e1011838. https://doi.org/10.1371/journal.ppat.1011838

Author

Kristensen, Charlotte ; Laybourn, Helena A. ; Crumpton, Jeri Carol ; Martiny, Karen ; Webb, Ashley ; Ryt-Hansen, Pia ; Trebbien, Ramona ; Jensen, Henrik E. ; Nissen, Jakob N. ; Skovgaard, Kerstin ; Webby, Richard J. ; Larsen, Lars E. / Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations. In: PLoS Pathogens. 2023 ; Vol. 19, No. 12.

Bibtex

@article{4fa8f00ab52b490faa4fa0de70383839,
title = "Experimental infection of pigs and ferrets with {"}pre-pandemic,{"} human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations",
abstract = "Influenza A viruses are RNA viruses that cause epidemics in humans and are enzootic in the pig population globally. In 2009, pig-to-human transmission of a reassortant H1N1 virus (H1N1pdm09) caused the first influenza pandemic of the 21st century. This study investigated the infection dynamics, pathogenesis, and lesions in pigs and ferrets inoculated with natural isolates of swine-adapted, human-adapted, and {"}pre-pandemic{"}H1N1pdm09 viruses. Additionally, the direct-contact and aerosol transmission properties of the three H1N1pdm09 isolates were assessed in ferrets. In pigs, inoculated ferrets, and ferrets infected by direct contact with inoculated ferrets, the pre-pandemic H1N1pdm09 virus induced an intermediary viral load, caused the most severe lesions, and had the highest clinical impact. The swine-adapted H1N1pdm09 virus induced the highest viral load, caused intermediary lesions, and had the least clinical impact in pigs. The human-adapted H1N1pdm09 virus induced the highest viral load, caused the mildest lesions, and had the least clinical impact in ferrets infected by direct contact. The discrepancy between viral load and clinical impact presumably reflects the importance of viral host adaptation. Interestingly, the swine-adapted H1N1pdm09 virus was transmitted by aerosols to two-thirds of the ferrets. Further work is needed to assess the risk of human-to-human aerosol transmission of swine-adapted H1N1pdm09 viruses.",
author = "Charlotte Kristensen and Laybourn, {Helena A.} and Crumpton, {Jeri Carol} and Karen Martiny and Ashley Webb and Pia Ryt-Hansen and Ramona Trebbien and Jensen, {Henrik E.} and Nissen, {Jakob N.} and Kerstin Skovgaard and Webby, {Richard J.} and Larsen, {Lars E.}",
note = "Publisher Copyright: {\textcopyright} 2023 Kristensen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2023",
doi = "10.1371/journal.ppat.1011838",
language = "English",
volume = "19",
journal = "P L o S Pathogens (Online)",
issn = "1553-7374",
publisher = "public library of science",
number = "12",

}

RIS

TY - JOUR

T1 - Experimental infection of pigs and ferrets with "pre-pandemic," human-adapted, and swineadapted variants of the H1N1pdm09 influenza A virus reveals significant differences in viral dynamics and pathological manifestations

AU - Kristensen, Charlotte

AU - Laybourn, Helena A.

AU - Crumpton, Jeri Carol

AU - Martiny, Karen

AU - Webb, Ashley

AU - Ryt-Hansen, Pia

AU - Trebbien, Ramona

AU - Jensen, Henrik E.

AU - Nissen, Jakob N.

AU - Skovgaard, Kerstin

AU - Webby, Richard J.

AU - Larsen, Lars E.

N1 - Publisher Copyright: © 2023 Kristensen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2023

Y1 - 2023

N2 - Influenza A viruses are RNA viruses that cause epidemics in humans and are enzootic in the pig population globally. In 2009, pig-to-human transmission of a reassortant H1N1 virus (H1N1pdm09) caused the first influenza pandemic of the 21st century. This study investigated the infection dynamics, pathogenesis, and lesions in pigs and ferrets inoculated with natural isolates of swine-adapted, human-adapted, and "pre-pandemic"H1N1pdm09 viruses. Additionally, the direct-contact and aerosol transmission properties of the three H1N1pdm09 isolates were assessed in ferrets. In pigs, inoculated ferrets, and ferrets infected by direct contact with inoculated ferrets, the pre-pandemic H1N1pdm09 virus induced an intermediary viral load, caused the most severe lesions, and had the highest clinical impact. The swine-adapted H1N1pdm09 virus induced the highest viral load, caused intermediary lesions, and had the least clinical impact in pigs. The human-adapted H1N1pdm09 virus induced the highest viral load, caused the mildest lesions, and had the least clinical impact in ferrets infected by direct contact. The discrepancy between viral load and clinical impact presumably reflects the importance of viral host adaptation. Interestingly, the swine-adapted H1N1pdm09 virus was transmitted by aerosols to two-thirds of the ferrets. Further work is needed to assess the risk of human-to-human aerosol transmission of swine-adapted H1N1pdm09 viruses.

AB - Influenza A viruses are RNA viruses that cause epidemics in humans and are enzootic in the pig population globally. In 2009, pig-to-human transmission of a reassortant H1N1 virus (H1N1pdm09) caused the first influenza pandemic of the 21st century. This study investigated the infection dynamics, pathogenesis, and lesions in pigs and ferrets inoculated with natural isolates of swine-adapted, human-adapted, and "pre-pandemic"H1N1pdm09 viruses. Additionally, the direct-contact and aerosol transmission properties of the three H1N1pdm09 isolates were assessed in ferrets. In pigs, inoculated ferrets, and ferrets infected by direct contact with inoculated ferrets, the pre-pandemic H1N1pdm09 virus induced an intermediary viral load, caused the most severe lesions, and had the highest clinical impact. The swine-adapted H1N1pdm09 virus induced the highest viral load, caused intermediary lesions, and had the least clinical impact in pigs. The human-adapted H1N1pdm09 virus induced the highest viral load, caused the mildest lesions, and had the least clinical impact in ferrets infected by direct contact. The discrepancy between viral load and clinical impact presumably reflects the importance of viral host adaptation. Interestingly, the swine-adapted H1N1pdm09 virus was transmitted by aerosols to two-thirds of the ferrets. Further work is needed to assess the risk of human-to-human aerosol transmission of swine-adapted H1N1pdm09 viruses.

U2 - 10.1371/journal.ppat.1011838

DO - 10.1371/journal.ppat.1011838

M3 - Journal article

C2 - 38048355

AN - SCOPUS:85180010480

VL - 19

JO - P L o S Pathogens (Online)

JF - P L o S Pathogens (Online)

SN - 1553-7374

IS - 12

M1 - e1011838

ER -

ID: 379169115