Dramatic increase in slaughter condemnations due to Escherichia coli ST23 and ST101 within the Danish broiler production
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Dramatic increase in slaughter condemnations due to Escherichia coli ST23 and ST101 within the Danish broiler production. / Kromann, Sofie; Baig, Sharmin; Olsen, Rikke Heidemann; Edslev, Sofie Marie; Thøfner, Ida; Bojesen, Anders Miki; Jensen, Henrik Elvang; Stegger, Marc.
In: Veterinary Microbiology, Vol. 280, 109696, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dramatic increase in slaughter condemnations due to Escherichia coli ST23 and ST101 within the Danish broiler production
AU - Kromann, Sofie
AU - Baig, Sharmin
AU - Olsen, Rikke Heidemann
AU - Edslev, Sofie Marie
AU - Thøfner, Ida
AU - Bojesen, Anders Miki
AU - Jensen, Henrik Elvang
AU - Stegger, Marc
N1 - Publisher Copyright: © 2023 The Authors
PY - 2023
Y1 - 2023
N2 - Escherichia coli constitutes a major challenge to poultry even when the prevalence of colibacillosis is low. Additionally, specific E. coli strains can severely enhance the detrimental effects on productivity, animal welfare and antimicrobial use. In 2019–2020, a dramatic increase in colibacillosis occurred among Danish broilers causing late-onset mortality and high slaughter condemnations. In the present study, the pathology and causative E. coli-types were characterised. Furthermore, the outbreak-related strains were compared to isolates from concurrent “background” colibacillosis. During the study, 1039 birds were subjected to a comprehensive post-mortem examination, and a total of 349 E. coli isolates were sequenced and characterised by multi-locus sequence typing, virulence and resistance gene presence, plasmid replicon content and phylogenetic analysis. Productivity data from outbreak flocks revealed a mortality of 6.34% ± 3.74 and a condemnation of 5.04% ± 3.67. Contrary, the numbers were 3.18% ± 1.57% and 1.02% ± 0.4 among non-outbreak flocks, respectively. Major lesions were cellulitis (46.82%), airsacculitis (67.63%), pericarditis (55.49%), perihepatitis (41.04%) and femoral head necrosis with physeal/metaphyseal involvement (44.51%). Among non-outbreak broilers, the prevalence was 4.46%, 7.64%, 7.01%, 3.82% and 8.28%, respectively. ST23 and ST101 dominated heavily in outbreak flocks, whereas non-outbreak related isolates consisted of various other STs. A low level of resistance markers was evident, except in few multidrug-resistant isolates. Within ST23 and ST101, 13 and 12 virulence genes were significantly over-represented compared to non-outbreak isolates. In conclusion, clonal lineages were documented as the cause of a devastating outbreak of colibacillosis with great prospects for future interventions.
AB - Escherichia coli constitutes a major challenge to poultry even when the prevalence of colibacillosis is low. Additionally, specific E. coli strains can severely enhance the detrimental effects on productivity, animal welfare and antimicrobial use. In 2019–2020, a dramatic increase in colibacillosis occurred among Danish broilers causing late-onset mortality and high slaughter condemnations. In the present study, the pathology and causative E. coli-types were characterised. Furthermore, the outbreak-related strains were compared to isolates from concurrent “background” colibacillosis. During the study, 1039 birds were subjected to a comprehensive post-mortem examination, and a total of 349 E. coli isolates were sequenced and characterised by multi-locus sequence typing, virulence and resistance gene presence, plasmid replicon content and phylogenetic analysis. Productivity data from outbreak flocks revealed a mortality of 6.34% ± 3.74 and a condemnation of 5.04% ± 3.67. Contrary, the numbers were 3.18% ± 1.57% and 1.02% ± 0.4 among non-outbreak flocks, respectively. Major lesions were cellulitis (46.82%), airsacculitis (67.63%), pericarditis (55.49%), perihepatitis (41.04%) and femoral head necrosis with physeal/metaphyseal involvement (44.51%). Among non-outbreak broilers, the prevalence was 4.46%, 7.64%, 7.01%, 3.82% and 8.28%, respectively. ST23 and ST101 dominated heavily in outbreak flocks, whereas non-outbreak related isolates consisted of various other STs. A low level of resistance markers was evident, except in few multidrug-resistant isolates. Within ST23 and ST101, 13 and 12 virulence genes were significantly over-represented compared to non-outbreak isolates. In conclusion, clonal lineages were documented as the cause of a devastating outbreak of colibacillosis with great prospects for future interventions.
KW - Antimicrobial resistance
KW - APEC
KW - Colibacillosis
KW - Comparative genomics
KW - Phylogenetic analysis
KW - Virulence factors
U2 - 10.1016/j.vetmic.2023.109696
DO - 10.1016/j.vetmic.2023.109696
M3 - Journal article
C2 - 36893553
AN - SCOPUS:85149647107
VL - 280
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
M1 - 109696
ER -
ID: 340114387