Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria

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Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria. / Jibril, Abdurrahman Hassan; Okeke, Iruka N.; Dalsgaard, Anders; Menéndez, Vanesa García; Olsen, John Elmerdahl.

In: Antibiotics, Vol. 10, No. 2, 99, 2021, p. 1-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jibril, AH, Okeke, IN, Dalsgaard, A, Menéndez, VG & Olsen, JE 2021, 'Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria', Antibiotics, vol. 10, no. 2, 99, pp. 1-22. https://doi.org/10.3390/antibiotics10020099

APA

Jibril, A. H., Okeke, I. N., Dalsgaard, A., Menéndez, V. G., & Olsen, J. E. (2021). Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria. Antibiotics, 10(2), 1-22. [99]. https://doi.org/10.3390/antibiotics10020099

Vancouver

Jibril AH, Okeke IN, Dalsgaard A, Menéndez VG, Olsen JE. Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria. Antibiotics. 2021;10(2):1-22. 99. https://doi.org/10.3390/antibiotics10020099

Author

Jibril, Abdurrahman Hassan ; Okeke, Iruka N. ; Dalsgaard, Anders ; Menéndez, Vanesa García ; Olsen, John Elmerdahl. / Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria. In: Antibiotics. 2021 ; Vol. 10, No. 2. pp. 1-22.

Bibtex

@article{9123d2535b1d47bcb7aca64ba2f2c255,
title = "Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria",
abstract = "Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventyfour isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential.",
keywords = "Nigeria, Plasmids, Poultry, Resistance, Salmonella, Salmonella genomic islands, Whole genome sequence",
author = "Jibril, {Abdurrahman Hassan} and Okeke, {Iruka N.} and Anders Dalsgaard and Men{\'e}ndez, {Vanesa Garc{\'i}a} and Olsen, {John Elmerdahl}",
year = "2021",
doi = "10.3390/antibiotics10020099",
language = "English",
volume = "10",
pages = "1--22",
journal = "Antibiotics",
issn = "2079-6382",
publisher = "M D P I AG",
number = "2",

}

RIS

TY - JOUR

T1 - Genomic analysis of antimicrobial resistance and resistance plasmids in salmonella serovars from poultry in Nigeria

AU - Jibril, Abdurrahman Hassan

AU - Okeke, Iruka N.

AU - Dalsgaard, Anders

AU - Menéndez, Vanesa García

AU - Olsen, John Elmerdahl

PY - 2021

Y1 - 2021

N2 - Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventyfour isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential.

AB - Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventyfour isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential.

KW - Nigeria

KW - Plasmids

KW - Poultry

KW - Resistance

KW - Salmonella

KW - Salmonella genomic islands

KW - Whole genome sequence

U2 - 10.3390/antibiotics10020099

DO - 10.3390/antibiotics10020099

M3 - Journal article

C2 - 33498344

AN - SCOPUS:85099965965

VL - 10

SP - 1

EP - 22

JO - Antibiotics

JF - Antibiotics

SN - 2079-6382

IS - 2

M1 - 99

ER -

ID: 256513741