Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci

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Biofilm Challenge : Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci. / Wallis, Jonathan K; Krömker, Volker; Paduch, Jan-Hendrik.

I: Foods, Bind 8, Nr. 2, 79, 2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Wallis, JK, Krömker, V & Paduch, J-H 2019, 'Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci', Foods, bind 8, nr. 2, 79. https://doi.org/10.3390/foods8020079

APA

Wallis, J. K., Krömker, V., & Paduch, J-H. (2019). Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci. Foods, 8(2), [79]. https://doi.org/10.3390/foods8020079

Vancouver

Wallis JK, Krömker V, Paduch J-H. Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci. Foods. 2019;8(2). 79. https://doi.org/10.3390/foods8020079

Author

Wallis, Jonathan K ; Krömker, Volker ; Paduch, Jan-Hendrik. / Biofilm Challenge : Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci. I: Foods. 2019 ; Bind 8, Nr. 2.

Bibtex

@article{41ddd5d1913b4b39a0d2a9fc72167bda,
title = "Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci",
abstract = "Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains-S. xylosus (35/07) and S. epidermidis (575/08)-belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones.",
author = "Wallis, {Jonathan K} and Volker Kr{\"o}mker and Jan-Hendrik Paduch",
year = "2019",
doi = "10.3390/foods8020079",
language = "English",
volume = "8",
journal = "Foods",
issn = "2304-8158",
publisher = "MDPI AG",
number = "2",

}

RIS

TY - JOUR

T1 - Biofilm Challenge

T2 - Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci

AU - Wallis, Jonathan K

AU - Krömker, Volker

AU - Paduch, Jan-Hendrik

PY - 2019

Y1 - 2019

N2 - Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains-S. xylosus (35/07) and S. epidermidis (575/08)-belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones.

AB - Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains-S. xylosus (35/07) and S. epidermidis (575/08)-belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones.

U2 - 10.3390/foods8020079

DO - 10.3390/foods8020079

M3 - Journal article

C2 - 30791514

VL - 8

JO - Foods

JF - Foods

SN - 2304-8158

IS - 2

M1 - 79

ER -

ID: 237051131