Chemotherapy modulates intestinal immune gene expression including surfactant Protein-D and deleted in malignant brain tumors 1 in piglets
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Chemotherapy modulates intestinal immune gene expression including surfactant Protein-D and deleted in malignant brain tumors 1 in piglets. / Rathe, Mathias; Thomassen, Mads; Shen, René L.; Pontoppidan, Peter E.L.; Husby, Steffen; Müller, Klaus; Kruse, Torben A; Sangild, Per T.
I: Chemotherapy, Bind 61, Nr. 4, 2016, s. 204-216.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Chemotherapy modulates intestinal immune gene expression including surfactant Protein-D and deleted in malignant brain tumors 1 in piglets
AU - Rathe, Mathias
AU - Thomassen, Mads
AU - Shen, René L.
AU - Pontoppidan, Peter E.L.
AU - Husby, Steffen
AU - Müller, Klaus
AU - Kruse, Torben A
AU - Sangild, Per T.
PY - 2016
Y1 - 2016
N2 - Background: Information about chemotherapy-induced intestinal gene expression may provide insight into the mechanisms underlying gut toxicity and help identify biomarkers and targets for intervention. Methods: We analyzed jejunal tissue from piglets subjected to two different, clinically relevant chemotherapy regimens: (1) busulfan plus cyclophosphamide (BUCY) and (2) doxorubicin (DOX). Results: Gene expression analysis identified 1,328 differentially expressed genes in the BUCY piglets and 594 in the DOX piglets, compared to controls. Similar changes in expression were found for 137 genes across the BUCY and DOX piglets. Selected genes of potential biological significance with a similar change in expression across the treatments were controlled by real-time polymerase chain reaction. Key innate defense molecules, including surfactant protein-D and deleted in malignant brain tumors 1, were among the upregulated genes for both treatments. Conclusion: In the developing intestine, chemotherapy increases the expression of genes related to innate immune functions involved in surveillance, protection, and homeostasis of mucosal surfaces.
AB - Background: Information about chemotherapy-induced intestinal gene expression may provide insight into the mechanisms underlying gut toxicity and help identify biomarkers and targets for intervention. Methods: We analyzed jejunal tissue from piglets subjected to two different, clinically relevant chemotherapy regimens: (1) busulfan plus cyclophosphamide (BUCY) and (2) doxorubicin (DOX). Results: Gene expression analysis identified 1,328 differentially expressed genes in the BUCY piglets and 594 in the DOX piglets, compared to controls. Similar changes in expression were found for 137 genes across the BUCY and DOX piglets. Selected genes of potential biological significance with a similar change in expression across the treatments were controlled by real-time polymerase chain reaction. Key innate defense molecules, including surfactant protein-D and deleted in malignant brain tumors 1, were among the upregulated genes for both treatments. Conclusion: In the developing intestine, chemotherapy increases the expression of genes related to innate immune functions involved in surveillance, protection, and homeostasis of mucosal surfaces.
KW - Chemotherapy-induced mucositis
KW - Deleted in malignant brain tumors 1
KW - Gastrointestinal toxicity
KW - Mucosal barrier injury
KW - Mucositis
KW - Peptidoglycan recognition protein 2
KW - Pig model
KW - Surfactant protein-D
U2 - 10.1159/000442938
DO - 10.1159/000442938
M3 - Journal article
C2 - 26886263
AN - SCOPUS:84958818302
VL - 61
SP - 204
EP - 216
JO - Chemotherapy
JF - Chemotherapy
SN - 0009-3157
IS - 4
ER -
ID: 178892412