Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses
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Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses. / Lazov, Christina M.; Lohse, Louise; Belsham, Graham J.; Rasmussen, Thomas Bruun; Bøtner, Anette.
I: Viruses, Bind 14, Nr. 12, 2751, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses
AU - Lazov, Christina M.
AU - Lohse, Louise
AU - Belsham, Graham J.
AU - Rasmussen, Thomas Bruun
AU - Bøtner, Anette
N1 - Publisher Copyright: © 2022 by the authors.
PY - 2022
Y1 - 2022
N2 - Porcine epidemic diarrhea virus (PEDV), belonging to the genus Alphacoronavirus, can cause serious disease in pigs of all ages, especially in suckling pigs. Differences in virulence have been observed between various strains of this virus. In this study, four pigs were inoculated with PEDV from Germany (intestine/intestinal content collected from pigs in 2016) and four pigs with PEDV from Italy (intestine/intestinal material collected from pigs in 2016). The pigs were re-inoculated with the same virus on multiple occasions to create a more robust infection and enhance the antibody responses. The clinical signs and pathological changes observed were generally mild. Two distinct peaks of virus excretion were seen in the group of pigs inoculated with the PEDV from Germany, while only one strong peak was seen for the group of pigs that received the virus from Italy. Seroconversion was seen by days 18 and 10 post-inoculation with PEDV in all surviving pigs from the groups that received the inoculums from Germany and Italy, respectively. Attempts to infect pigs with a swine enteric coronavirus (SeCoV) from Slovakia were unsuccessful, and no signs of infection were observed in the inoculated animals.
AB - Porcine epidemic diarrhea virus (PEDV), belonging to the genus Alphacoronavirus, can cause serious disease in pigs of all ages, especially in suckling pigs. Differences in virulence have been observed between various strains of this virus. In this study, four pigs were inoculated with PEDV from Germany (intestine/intestinal content collected from pigs in 2016) and four pigs with PEDV from Italy (intestine/intestinal material collected from pigs in 2016). The pigs were re-inoculated with the same virus on multiple occasions to create a more robust infection and enhance the antibody responses. The clinical signs and pathological changes observed were generally mild. Two distinct peaks of virus excretion were seen in the group of pigs inoculated with the PEDV from Germany, while only one strong peak was seen for the group of pigs that received the virus from Italy. Seroconversion was seen by days 18 and 10 post-inoculation with PEDV in all surviving pigs from the groups that received the inoculums from Germany and Italy, respectively. Attempts to infect pigs with a swine enteric coronavirus (SeCoV) from Slovakia were unsuccessful, and no signs of infection were observed in the inoculated animals.
KW - coronavirus
KW - experimental infection
KW - porcine epidemic diarrhea virus (PEDV)
KW - swine enteric coronavirus (SeCoV)
U2 - 10.3390/v14122751
DO - 10.3390/v14122751
M3 - Journal article
C2 - 36560755
AN - SCOPUS:85144540859
VL - 14
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 12
M1 - 2751
ER -
ID: 330900254