Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development

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Standard

Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development. / Hall, Vanessa Jane; Christensen, Josef; Gao, Yu; Schmidt, Mette; Hyttel, Poul.

I: Developmental Dynamics, Bind 238, Nr. 8, 2009, s. 2014-2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hall, VJ, Christensen, J, Gao, Y, Schmidt, M & Hyttel, P 2009, 'Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development', Developmental Dynamics, bind 238, nr. 8, s. 2014-2024. https://doi.org/10.1002/dvdy.22027

APA

Hall, V. J., Christensen, J., Gao, Y., Schmidt, M., & Hyttel, P. (2009). Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development. Developmental Dynamics, 238(8), 2014-2024. https://doi.org/10.1002/dvdy.22027

Vancouver

Hall VJ, Christensen J, Gao Y, Schmidt M, Hyttel P. Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development. Developmental Dynamics. 2009;238(8):2014-2024. https://doi.org/10.1002/dvdy.22027

Author

Hall, Vanessa Jane ; Christensen, Josef ; Gao, Yu ; Schmidt, Mette ; Hyttel, Poul. / Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development. I: Developmental Dynamics. 2009 ; Bind 238, Nr. 8. s. 2014-2024.

Bibtex

@article{e8231d609d3011debc73000ea68e967b,
title = "Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development",
abstract = "  The signaling mechanisms regulating pluripotency in porcine embryonic stem cells and embryos are unknown. In this study, we characterize cell signaling in the in-vivo porcine inner cell mass and later-stage epiblast. We evaluate expression of OCT4, NANOG, SOX2, genes within the JAK/STAT pathway (LIF, LIFR, GP130), FGF pathway (bFGF, FGFR1, FGFR2), BMP pathway (BMP4), and downstream-activated genes (STAT3, c-Myc, c-Fos, and SMAD4). We discovered two different expression profiles exist in the developing porcine embryo. The D6 porcine blastocyst (inner cell mass stage) is devoid in the expression of most genes analyzed, with the exception of OCT4. In contrast, the D11 epiblast expressed 10 of the 12 genes investigated. Immunocytochemistry confirmed LIFR and bFGF was not expressed in the epiblast, but within the trophectoderm. These findings reveal cell signaling associated with maintaining pluripotency in human embryonic stem cells is detectable in the porcine epiblast, but not in the inner cell mass. Copyright (c) 2009 Wiley-Liss, Inc.",
author = "Hall, {Vanessa Jane} and Josef Christensen and Yu Gao and Mette Schmidt and Poul Hyttel",
year = "2009",
doi = "10.1002/dvdy.22027",
language = "English",
volume = "238",
pages = "2014--2024",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "JohnWiley & Sons, Inc.",
number = "8",

}

RIS

TY - JOUR

T1 - Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development

AU - Hall, Vanessa Jane

AU - Christensen, Josef

AU - Gao, Yu

AU - Schmidt, Mette

AU - Hyttel, Poul

PY - 2009

Y1 - 2009

N2 -   The signaling mechanisms regulating pluripotency in porcine embryonic stem cells and embryos are unknown. In this study, we characterize cell signaling in the in-vivo porcine inner cell mass and later-stage epiblast. We evaluate expression of OCT4, NANOG, SOX2, genes within the JAK/STAT pathway (LIF, LIFR, GP130), FGF pathway (bFGF, FGFR1, FGFR2), BMP pathway (BMP4), and downstream-activated genes (STAT3, c-Myc, c-Fos, and SMAD4). We discovered two different expression profiles exist in the developing porcine embryo. The D6 porcine blastocyst (inner cell mass stage) is devoid in the expression of most genes analyzed, with the exception of OCT4. In contrast, the D11 epiblast expressed 10 of the 12 genes investigated. Immunocytochemistry confirmed LIFR and bFGF was not expressed in the epiblast, but within the trophectoderm. These findings reveal cell signaling associated with maintaining pluripotency in human embryonic stem cells is detectable in the porcine epiblast, but not in the inner cell mass. Copyright (c) 2009 Wiley-Liss, Inc.

AB -   The signaling mechanisms regulating pluripotency in porcine embryonic stem cells and embryos are unknown. In this study, we characterize cell signaling in the in-vivo porcine inner cell mass and later-stage epiblast. We evaluate expression of OCT4, NANOG, SOX2, genes within the JAK/STAT pathway (LIF, LIFR, GP130), FGF pathway (bFGF, FGFR1, FGFR2), BMP pathway (BMP4), and downstream-activated genes (STAT3, c-Myc, c-Fos, and SMAD4). We discovered two different expression profiles exist in the developing porcine embryo. The D6 porcine blastocyst (inner cell mass stage) is devoid in the expression of most genes analyzed, with the exception of OCT4. In contrast, the D11 epiblast expressed 10 of the 12 genes investigated. Immunocytochemistry confirmed LIFR and bFGF was not expressed in the epiblast, but within the trophectoderm. These findings reveal cell signaling associated with maintaining pluripotency in human embryonic stem cells is detectable in the porcine epiblast, but not in the inner cell mass. Copyright (c) 2009 Wiley-Liss, Inc.

U2 - 10.1002/dvdy.22027

DO - 10.1002/dvdy.22027

M3 - Journal article

C2 - 19618464

VL - 238

SP - 2014

EP - 2024

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 8

ER -

ID: 14307073