Roles of skeletal muscle morphology and activity in determining Na+-K+- ATPase concentration in young pigs
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Roles of skeletal muscle morphology and activity in determining Na+-K+- ATPase concentration in young pigs. / Harrison, A. P.; Clausen, T.; Duchamp, C.; Dauncey, M. J.
I: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Bind 266, Nr. 1 35-1, 1994, s. R102-R111.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Roles of skeletal muscle morphology and activity in determining Na+-K+- ATPase concentration in young pigs
AU - Harrison, A. P.
AU - Clausen, T.
AU - Duchamp, C.
AU - Dauncey, M. J.
PY - 1994
Y1 - 1994
N2 - It has been demonstrated previously that the Na+-K+- adenosinetriphosphatase (Na+-K+-ATPase) concentration (total concentration of [3H]ouabain-binding sites = maximal [3H]ouabain-binding capacity, B(max)) in longissimus dorsi muscle is higher in young pigs living in a cold than in a warm environment, with the effect being even greater in those on a low rather than a high energy intake in the cold. The importance of thyroid status, myofiber type and size, and muscular activity in determining the concentration of Na+-K+-ATPase has therefore been assessed in longissimus dorsi, semitendinosus, and soleus muscles from 8-wk-old littermate pigs, which had been living 35 or 10°C on either a high or a low energy intake for 3-4 wk. Changes in neither plasma thyroid hormone concentrations nor myofiber type could account for the greater B(max) at 10 vs. 35°C observed in all three muscles; instead, it was possibly related to the greater muscular activity associated with shivering in the cold. More than 50% of the increase in B(max) on the low compared with the high intake in the cold could be attributed to the smaller fiber size and hence greater membrane area in animals on the low intake, and it is concluded that membrane surface area must be assessed in studies of membrane-bound enzymes.
AB - It has been demonstrated previously that the Na+-K+- adenosinetriphosphatase (Na+-K+-ATPase) concentration (total concentration of [3H]ouabain-binding sites = maximal [3H]ouabain-binding capacity, B(max)) in longissimus dorsi muscle is higher in young pigs living in a cold than in a warm environment, with the effect being even greater in those on a low rather than a high energy intake in the cold. The importance of thyroid status, myofiber type and size, and muscular activity in determining the concentration of Na+-K+-ATPase has therefore been assessed in longissimus dorsi, semitendinosus, and soleus muscles from 8-wk-old littermate pigs, which had been living 35 or 10°C on either a high or a low energy intake for 3-4 wk. Changes in neither plasma thyroid hormone concentrations nor myofiber type could account for the greater B(max) at 10 vs. 35°C observed in all three muscles; instead, it was possibly related to the greater muscular activity associated with shivering in the cold. More than 50% of the increase in B(max) on the low compared with the high intake in the cold could be attributed to the smaller fiber size and hence greater membrane area in animals on the low intake, and it is concluded that membrane surface area must be assessed in studies of membrane-bound enzymes.
KW - development
KW - energy
KW - environment
KW - growth
KW - muscle fiber type
KW - muscular activity
KW - ouabain binding
KW - sodium-potassium pump
KW - thermogenesis
KW - thyroid hormones
UR - http://www.scopus.com/inward/record.url?scp=0028118534&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.1994.266.1.r102
DO - 10.1152/ajpregu.1994.266.1.r102
M3 - Journal article
C2 - 8304530
AN - SCOPUS:0028118534
VL - 266
SP - R102-R111
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 1 35-1
ER -
ID: 316852316