Polymerization-dependent activation of porcine γδ T-cells by proanthocyanidins
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Polymerization-dependent activation of porcine γδ T-cells by proanthocyanidins. / Williams, Andrew Richard; Fryganas, Christos; Reichwald, Kirsten; Skov, Søren; Mueller-Harvey, Irene; Thamsborg, Stig Milan.
In: Research in Veterinary Science, Vol. 105, 2016, p. 209-215.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Polymerization-dependent activation of porcine γδ T-cells by proanthocyanidins
AU - Williams, Andrew Richard
AU - Fryganas, Christos
AU - Reichwald, Kirsten
AU - Skov, Søren
AU - Mueller-Harvey, Irene
AU - Thamsborg, Stig Milan
N1 - Copyright © 2016 Elsevier Ltd. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Plant-derived proanthocyanidins (PAC) have been promoted as a natural method of improving health and immune function in livestock. It has previously been shown that PAC are effective agonists for activating ruminant γδ T-cells in vitro, however effects on other livestock species are not yet clear. Moreover, the fine structural characteristics of the PAC which contribute to this stimulatory effect have not been elucidated. Here, we demonstrate activation of porcine γδ T-cells by PAC via up-regulation of CD25 (IL-2Rα) and show that 1) activation is dependent on degree of polymerization (DP), with PAC fractions containing polymers with mean DP >6 significantly more effective than fractions with mean DP <6, whilst flavan-3-ol monomers (the constituent monomeric units of PAC) did not induce CD25 expression and 2) both procyanidin and prodelphinidin-type PAC are effective agonists. Furthermore, we show that this effect of PAC is restricted to the γδ T-cell population within porcine peripheral mononuclear cells as significant CD25 up-regulation was not observed in non γδ T-cells, and no activation (via CD80/86 up-regulation) was evident in monocytes. Our results show that dietary PAC may contribute to enhancement of innate immunity in swine via activation of γδ T-cells.
AB - Plant-derived proanthocyanidins (PAC) have been promoted as a natural method of improving health and immune function in livestock. It has previously been shown that PAC are effective agonists for activating ruminant γδ T-cells in vitro, however effects on other livestock species are not yet clear. Moreover, the fine structural characteristics of the PAC which contribute to this stimulatory effect have not been elucidated. Here, we demonstrate activation of porcine γδ T-cells by PAC via up-regulation of CD25 (IL-2Rα) and show that 1) activation is dependent on degree of polymerization (DP), with PAC fractions containing polymers with mean DP >6 significantly more effective than fractions with mean DP <6, whilst flavan-3-ol monomers (the constituent monomeric units of PAC) did not induce CD25 expression and 2) both procyanidin and prodelphinidin-type PAC are effective agonists. Furthermore, we show that this effect of PAC is restricted to the γδ T-cell population within porcine peripheral mononuclear cells as significant CD25 up-regulation was not observed in non γδ T-cells, and no activation (via CD80/86 up-regulation) was evident in monocytes. Our results show that dietary PAC may contribute to enhancement of innate immunity in swine via activation of γδ T-cells.
U2 - 10.1016/j.rvsc.2016.02.021
DO - 10.1016/j.rvsc.2016.02.021
M3 - Journal article
C2 - 27033935
VL - 105
SP - 209
EP - 215
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
ER -
ID: 161246858