Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun?

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun? / Olesen, Ann Sofie; Stelder, Jonno Jorn; Tjørnehøj, Kirsten; Johnston, Camille Melissa; Lohse, Louise; Kjær, Lene Jung; Boklund, Anette Ella; Bøtner, Anette; Belsham, Graham J.; Bødker, René; Rasmussen, Thomas Bruun.

In: Viruses, Vol. 15, No. 6, 1255, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olesen, AS, Stelder, JJ, Tjørnehøj, K, Johnston, CM, Lohse, L, Kjær, LJ, Boklund, AE, Bøtner, A, Belsham, GJ, Bødker, R & Rasmussen, TB 2023, 'Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun?', Viruses, vol. 15, no. 6, 1255. https://doi.org/10.3390/v15061255

APA

Olesen, A. S., Stelder, J. J., Tjørnehøj, K., Johnston, C. M., Lohse, L., Kjær, L. J., Boklund, A. E., Bøtner, A., Belsham, G. J., Bødker, R., & Rasmussen, T. B. (2023). Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun? Viruses, 15(6), [1255]. https://doi.org/10.3390/v15061255

Vancouver

Olesen AS, Stelder JJ, Tjørnehøj K, Johnston CM, Lohse L, Kjær LJ et al. Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun? Viruses. 2023;15(6). 1255. https://doi.org/10.3390/v15061255

Author

Olesen, Ann Sofie ; Stelder, Jonno Jorn ; Tjørnehøj, Kirsten ; Johnston, Camille Melissa ; Lohse, Louise ; Kjær, Lene Jung ; Boklund, Anette Ella ; Bøtner, Anette ; Belsham, Graham J. ; Bødker, René ; Rasmussen, Thomas Bruun. / Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun?. In: Viruses. 2023 ; Vol. 15, No. 6.

Bibtex

@article{47bc89fd6a4f4bbd8a1049de93b785da,
title = "Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun?",
abstract = "A seasonal trend of African swine fever (ASF) outbreaks in domestic pig farms has been observed in affected regions of Eastern Europe. Most outbreaks have been observed during the warmer summer months, coinciding with the seasonal activity pattern of blood-feeding insects. These insects may offer a route for introduction of the ASF virus (ASFV) into domestic pig herds. In this study, insects (hematophagous flies) collected outside the buildings of a domestic pig farm, without ASFV-infected pigs, were analyzed for the presence of the virus. Using qPCR, ASFV DNA was detected in six insect pools; in four of these pools, DNA from suid blood was also identified. This detection coincided with ASFV being reported in the wild boar population within a 10 km radius of the pig farm. These findings show that blood from ASFV-infected suids was present within hematophagous flies on the premises of a pig farm without infected animals and support the hypothesis that blood-feeding insects can potentially transport the virus from wild boars into domestic pig farms.",
keywords = "ASF, hematophagous insects, high-biosecurity farm, vector, virus introduction, virus transmission",
author = "Olesen, {Ann Sofie} and Stelder, {Jonno Jorn} and Kirsten Tj{\o}rneh{\o}j and Johnston, {Camille Melissa} and Louise Lohse and Kj{\ae}r, {Lene Jung} and Boklund, {Anette Ella} and Anette B{\o}tner and Belsham, {Graham J.} and Ren{\'e} B{\o}dker and Rasmussen, {Thomas Bruun}",
note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",
year = "2023",
doi = "10.3390/v15061255",
language = "English",
volume = "15",
journal = "Viruses",
issn = "1999-4915",
publisher = "M D P I AG",
number = "6",

}

RIS

TY - JOUR

T1 - Detection of African Swine Fever Virus and Blood Meals of Porcine Origin in Hematophagous Insects Collected Adjacent to a High-Biosecurity Pig Farm in Lithuania; A Smoking Gun?

AU - Olesen, Ann Sofie

AU - Stelder, Jonno Jorn

AU - Tjørnehøj, Kirsten

AU - Johnston, Camille Melissa

AU - Lohse, Louise

AU - Kjær, Lene Jung

AU - Boklund, Anette Ella

AU - Bøtner, Anette

AU - Belsham, Graham J.

AU - Bødker, René

AU - Rasmussen, Thomas Bruun

N1 - Publisher Copyright: © 2023 by the authors.

PY - 2023

Y1 - 2023

N2 - A seasonal trend of African swine fever (ASF) outbreaks in domestic pig farms has been observed in affected regions of Eastern Europe. Most outbreaks have been observed during the warmer summer months, coinciding with the seasonal activity pattern of blood-feeding insects. These insects may offer a route for introduction of the ASF virus (ASFV) into domestic pig herds. In this study, insects (hematophagous flies) collected outside the buildings of a domestic pig farm, without ASFV-infected pigs, were analyzed for the presence of the virus. Using qPCR, ASFV DNA was detected in six insect pools; in four of these pools, DNA from suid blood was also identified. This detection coincided with ASFV being reported in the wild boar population within a 10 km radius of the pig farm. These findings show that blood from ASFV-infected suids was present within hematophagous flies on the premises of a pig farm without infected animals and support the hypothesis that blood-feeding insects can potentially transport the virus from wild boars into domestic pig farms.

AB - A seasonal trend of African swine fever (ASF) outbreaks in domestic pig farms has been observed in affected regions of Eastern Europe. Most outbreaks have been observed during the warmer summer months, coinciding with the seasonal activity pattern of blood-feeding insects. These insects may offer a route for introduction of the ASF virus (ASFV) into domestic pig herds. In this study, insects (hematophagous flies) collected outside the buildings of a domestic pig farm, without ASFV-infected pigs, were analyzed for the presence of the virus. Using qPCR, ASFV DNA was detected in six insect pools; in four of these pools, DNA from suid blood was also identified. This detection coincided with ASFV being reported in the wild boar population within a 10 km radius of the pig farm. These findings show that blood from ASFV-infected suids was present within hematophagous flies on the premises of a pig farm without infected animals and support the hypothesis that blood-feeding insects can potentially transport the virus from wild boars into domestic pig farms.

KW - ASF

KW - hematophagous insects

KW - high-biosecurity farm

KW - vector

KW - virus introduction

KW - virus transmission

U2 - 10.3390/v15061255

DO - 10.3390/v15061255

M3 - Journal article

C2 - 37376554

AN - SCOPUS:85164032070

VL - 15

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 6

M1 - 1255

ER -

ID: 362700303