Reservoirs of Corynebacterium spp. in the Environment of Dairy Cows
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Reservoirs of Corynebacterium spp. in the Environment of Dairy Cows. / Woudstra, Svenja; Lücken, Anneke; Wente, Nicole; Zhang, Yanchao; Leimbach, Stefanie; Gussmann, Maya Katrin; Kirkeby, Carsten; Krömker, Volker.
In: Pathogens, Vol. 12, No. 1, 139, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Reservoirs of Corynebacterium spp. in the Environment of Dairy Cows
AU - Woudstra, Svenja
AU - Lücken, Anneke
AU - Wente, Nicole
AU - Zhang, Yanchao
AU - Leimbach, Stefanie
AU - Gussmann, Maya Katrin
AU - Kirkeby, Carsten
AU - Krömker, Volker
PY - 2023
Y1 - 2023
N2 - Although Corynebacterium spp. can be regularly associated with subclinical and clinical mastitis cases in dairy cows, knowledge on their reservoirs in dairy farms is sparse. Therefore, samples were collected at 10 visits with 14 day intervals from bedding material (n = 50), drinking troughs (n = 20), different walking areas (n = 60), cow brushes (n = 8), fly traps (n = 4), the passage to pasture (n = 9) as well as milking liners (n = 80) and milker gloves (n = 20) in one dairy cow farm. Additionally, quarter foremilk samples from all lactating cows (approximately 200) were collected at each visit. All samples underwent microbiological examination and cultured isolates were identified using MALDI-TOF MS. Most Corynebacterium spp. that were cultivated from milk were also isolated from the housing environment and milking-related niches (C. amycolatum, C. confusum, C. stationis, C. variabile, C. xerosis) or from milking-related niches only (C. frankenforstense, C. pilosum, C. suicordis). C. bovis was not cultivated from any environmental niche, while being the dominant species in milk samples. This study demonstrates that many Corynebacterium spp. present in milk samples can also be isolated from the cows’ environment. For C. bovis, the most relevant Corynebacterium species with regard to intramammary infections, it indicates that environmental reservoirs are of little relevance.
AB - Although Corynebacterium spp. can be regularly associated with subclinical and clinical mastitis cases in dairy cows, knowledge on their reservoirs in dairy farms is sparse. Therefore, samples were collected at 10 visits with 14 day intervals from bedding material (n = 50), drinking troughs (n = 20), different walking areas (n = 60), cow brushes (n = 8), fly traps (n = 4), the passage to pasture (n = 9) as well as milking liners (n = 80) and milker gloves (n = 20) in one dairy cow farm. Additionally, quarter foremilk samples from all lactating cows (approximately 200) were collected at each visit. All samples underwent microbiological examination and cultured isolates were identified using MALDI-TOF MS. Most Corynebacterium spp. that were cultivated from milk were also isolated from the housing environment and milking-related niches (C. amycolatum, C. confusum, C. stationis, C. variabile, C. xerosis) or from milking-related niches only (C. frankenforstense, C. pilosum, C. suicordis). C. bovis was not cultivated from any environmental niche, while being the dominant species in milk samples. This study demonstrates that many Corynebacterium spp. present in milk samples can also be isolated from the cows’ environment. For C. bovis, the most relevant Corynebacterium species with regard to intramammary infections, it indicates that environmental reservoirs are of little relevance.
U2 - 10.3390/pathogens12010139
DO - 10.3390/pathogens12010139
M3 - Journal article
C2 - 36678487
VL - 12
JO - Pathogens
JF - Pathogens
SN - 2076-0817
IS - 1
M1 - 139
ER -
ID: 333617397