EARLY ENTERAL FEEDING REDUCES SEPSIS RESPONSE AND NEUROINFLAMMATION IN A PIG MODEL OF NEONATAL BLOODSTREAM INFECTION
Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
Background and aims
Preterm birth and infections account for the majority of early childhood morbidity. Neonatal sepsis remains a major clinical and societal burden due to
associated neurodevelopmental disturbances. Milk contains several immunologically active compounds and may support infection resistance. Using a novel preterm piglet model of bloodstream infection, we hypothesized that early enteral milk feeding supports resistance against sepsis and neuroinflammation.
Methods
Forty cesarean born preterm piglets were administered saline (CON) or
Staphylococcus epidermidis (SE, 109 CFU/kg) systemically within 6 hours
of birth and given total parenteral nutrition (CON-TPN, n=11; SE-TPN,
n=15) or enteral nutrition (SE-ENT, n=14, 9 ml/kg/3h cow’s colostrum).
After 24 hours, we recorded bacteriology, hematology, hemodynamics,
thromboelastographies (TEGs), and acid–base balance. Furthermore, in vivo
blood-cerebrospinal fluid (CSF) barrier permeability, CSF leukocyte count,
microglia numbers and brain innate immune gene expression were assessed.
Results
ENT-SE pigs had fewer bacteria than TPN-SE in blood and CSF (both
p<0.05), and physical activity levels, blood pressure, blood lactate and
acidity were similar to CON-TPN, while TPN-SE pigs showed decreased
physical activity, blood pressure and blood pH, and increased lactate (all
p<0.05). Lymphopenia, thrombocytopenia and procoagulant TEG profiles
were observed in both SE-infected groups. In vivo blood-CSF barrier
permeability and CSF leukocytes were lower in ENT-SE than TPN-SE
pigs (both p<0.05). Moreover, the ENT treatment downregulated several
immune-related genes in the brain, while microglia numbers were increased
in both SE-infected groups.
Conclusions
Using a newly established clinically relevant animal model of neonatal
bloodstream infection, we show that early enteral feeding confers resistance
against sepsis and neuroinflammation
Original language | English |
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Article number | 307, EAPS-0930 |
Journal | European Journal of Pediatrics |
Volume | 175 |
Issue number | 11 |
Pages (from-to) | 1497-1497 |
Number of pages | 1 |
ISSN | 0340-6199 |
DOIs | |
Publication status | Published - Nov 2016 |
Event | Congress of the European Academy of Paediatric Societies - Palexpo, Geneva, Switzerland Duration: 21 Oct 2016 → 25 Oct 2016 Conference number: 6 |
Conference
Conference | Congress of the European Academy of Paediatric Societies |
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Number | 6 |
Location | Palexpo |
Country | Switzerland |
City | Geneva |
Period | 21/10/2016 → 25/10/2016 |
Bibliographical note
EAPS-0930
ID: 172269839