Identification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicity
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Identification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicity. / Wong, Leo Lap Yan; Fan, Sheung Tat; Man, Kwan; Sit, Wai Hung; Jiang, Ping Ping; Jor, Irene Wing Yan; Lee, Carol Yee Ki; Ling, Wai Lim; Tam, Kin Tung; Wan, Jennifer Man Fan.
In: Human and Experimental Toxicology, Vol. 30, No. 9, 09.2011, p. 1369-1381.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicity
AU - Wong, Leo Lap Yan
AU - Fan, Sheung Tat
AU - Man, Kwan
AU - Sit, Wai Hung
AU - Jiang, Ping Ping
AU - Jor, Irene Wing Yan
AU - Lee, Carol Yee Ki
AU - Ling, Wai Lim
AU - Tam, Kin Tung
AU - Wan, Jennifer Man Fan
PY - 2011/9
Y1 - 2011/9
N2 - Carbon tetrachloride (CCl4) is a common hepatotoxin used in experimental models to elicit liver injury. To identify the proteins involved in CCl4-induced hepatotoxicity, two-dimensional gel electrophoresis was employed followed by mass spectrometry - mass spectrometry (MS/MS) to study the differentially expressed proteins during CCl4 exposure in the Fischer 344 rat liver proteome for 5 weeks. Ten spots with notable changes between the Control and CCl4 groups were successfully identified. Among them, four proteins with significant up-regulation, namely calcium-binding protein 1, protein disulfide isomerase, mitochondrial aldehyde dehydrogenase precursor, and, glutathione-S-transferase mu1 and six proteins with significant down-regulation, namely catechol-O-methyltransferase, hemoglobin-alpha-2-chain, hemopexin precursor, methionine sulfoxide reductase A, catalase and carbonic anhydrase 3, were identified. The data indicates that CCl4 causes hepatotoxicity by depleting oxygen radical scavengers in the hepatocytes. In this rat model, we profiled hepatic proteome alterations in response to CCl4 intoxication. The findings should facilitate understanding of the mechanism of CCl4-induced liver injury.
AB - Carbon tetrachloride (CCl4) is a common hepatotoxin used in experimental models to elicit liver injury. To identify the proteins involved in CCl4-induced hepatotoxicity, two-dimensional gel electrophoresis was employed followed by mass spectrometry - mass spectrometry (MS/MS) to study the differentially expressed proteins during CCl4 exposure in the Fischer 344 rat liver proteome for 5 weeks. Ten spots with notable changes between the Control and CCl4 groups were successfully identified. Among them, four proteins with significant up-regulation, namely calcium-binding protein 1, protein disulfide isomerase, mitochondrial aldehyde dehydrogenase precursor, and, glutathione-S-transferase mu1 and six proteins with significant down-regulation, namely catechol-O-methyltransferase, hemoglobin-alpha-2-chain, hemopexin precursor, methionine sulfoxide reductase A, catalase and carbonic anhydrase 3, were identified. The data indicates that CCl4 causes hepatotoxicity by depleting oxygen radical scavengers in the hepatocytes. In this rat model, we profiled hepatic proteome alterations in response to CCl4 intoxication. The findings should facilitate understanding of the mechanism of CCl4-induced liver injury.
KW - carbon tetrachloride
KW - hepatotoxicity
KW - liver
KW - proteomics
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=80052521476&partnerID=8YFLogxK
U2 - 10.1177/0960327110391388
DO - 10.1177/0960327110391388
M3 - Journal article
C2 - 21138988
AN - SCOPUS:80052521476
VL - 30
SP - 1369
EP - 1381
JO - Human and Experimental Toxicology
JF - Human and Experimental Toxicology
SN - 0960-3271
IS - 9
ER -
ID: 299106777