Plasma metabolomics to evaluate progression of necrotising enterocolitis in preterm pigs
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Plasma metabolomics to evaluate progression of necrotising enterocolitis in preterm pigs. / Jiang, Yan Nan; Ye, Yong Xin; Sangild, Per Torp; Thymann, Thomas; Engelsen, Søren Balling; Khakimov, Bekzod; Jiang, Ping Ping.
In: Metabolites, Vol. 11, No. 5, 283, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plasma metabolomics to evaluate progression of necrotising enterocolitis in preterm pigs
AU - Jiang, Yan Nan
AU - Ye, Yong Xin
AU - Sangild, Per Torp
AU - Thymann, Thomas
AU - Engelsen, Søren Balling
AU - Khakimov, Bekzod
AU - Jiang, Ping Ping
N1 - Publisher Copyright: © 2021 by the authors.
PY - 2021
Y1 - 2021
N2 - Necrotising enterocolitis (NEC) is a microbiome-dependent gut disease in preterm infants in early life. Antibiotic treatment is a common intervention for NEC. How NEC lesions, with or without antibiotics, affect plasma metabolome was explored in this study. Formula-fed preterm pigs were used as a model for human NEC and treated with saline, parenteral or oral antibiotics (n = 15-17) for four days after delivery. Gut tissues were collected for evaluation of NEC-like lesions and plasma for metabolomic analysis by proton nuclear magnetic resonance spectroscopy (1H-NMR). Metabolites were annotated, quantified and subjected to statistical modelling to delineate the effects of NEC and antibiotic treatment. Presence of severe NEC lesions, not antibiotic treatment, was the main drive for plasma metabolite changes. Relative to other pigs, pigs with severe NEC lesions had higher levels of alanine, histidine and myo-inositol, and lower levels of 3-hydroxybutyric acid and isobutyric acid. Across NEC lesion states (healthy, mild, severe), antibiotics directly affected only a few metabolites (tryptophan, 3-phenyllactic acid). Together and independently, NEC and antibiotic treatment affected circulating metabolites in preterm pigs. Amino acids and plasma metabolites, partly related to the gut microbiome, may be helpful to monitor progression of NEC lesions after proper validation.
AB - Necrotising enterocolitis (NEC) is a microbiome-dependent gut disease in preterm infants in early life. Antibiotic treatment is a common intervention for NEC. How NEC lesions, with or without antibiotics, affect plasma metabolome was explored in this study. Formula-fed preterm pigs were used as a model for human NEC and treated with saline, parenteral or oral antibiotics (n = 15-17) for four days after delivery. Gut tissues were collected for evaluation of NEC-like lesions and plasma for metabolomic analysis by proton nuclear magnetic resonance spectroscopy (1H-NMR). Metabolites were annotated, quantified and subjected to statistical modelling to delineate the effects of NEC and antibiotic treatment. Presence of severe NEC lesions, not antibiotic treatment, was the main drive for plasma metabolite changes. Relative to other pigs, pigs with severe NEC lesions had higher levels of alanine, histidine and myo-inositol, and lower levels of 3-hydroxybutyric acid and isobutyric acid. Across NEC lesion states (healthy, mild, severe), antibiotics directly affected only a few metabolites (tryptophan, 3-phenyllactic acid). Together and independently, NEC and antibiotic treatment affected circulating metabolites in preterm pigs. Amino acids and plasma metabolites, partly related to the gut microbiome, may be helpful to monitor progression of NEC lesions after proper validation.
KW - Amino acids
KW - Antibiotics
KW - Lipid metabolism
KW - Metabolomics
KW - Necrotising enterocolitis (NEC)
U2 - 10.3390/metabo11050283
DO - 10.3390/metabo11050283
M3 - Journal article
C2 - 33946896
AN - SCOPUS:85105611069
VL - 11
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 5
M1 - 283
ER -
ID: 269605705