Enzootic and zoonotic virus research: The Lars Erik Larsen group
The overarching goal of our work is to contribute to increased animal welfare and food safety in the Danish animal production. The core research areas are enzootic viral diseases in pigs and other production animals and zoonotic viruses in pigs and birds.
Influenza A virus (IAV) is an important pathogen in humans and animals and has zoonotic potential. Pigs are regarded as a melting pot for generation of new reassortant viruses potentially with zoonotic and pandemic capabilities.
“Thus, more knowledge on viral and host molecular determinants of host range restriction of new influenza A virus strains discovered in animals are urgently needed”, says research group leader Professor Lars Erik Larsen .
We want to extend our previous work to study the basic mechanism of host range determinants and virulence of influenza A viruses. We will establish in vitro and ex vivo cell and tissue cultures that allow the study of influenza virus/host interaction and infection dynamics and further develop the previous
Of scientifical highlights, my group was the first to demonstrate that the distribution of siliac acid receptors in pigs are similar to humans and that avian influenza virus primary replicates in type 2 pneumocytes of svine similar to humans.
During the last four years, we have performed a range of studies that emphatized that swIAV is a major veterinary pathogen in all age groups including piglets and that the virus persists in the herds and is subjected to significant antigenic and genetic evolution comparable to human influenza viruses. Furthermore, we have documented that the effect of vaccination and pre-existing immunity not only lack protective effects, but even may contribute to viral persistence. We want to focus on exploring the basic mechanism behind this by design of experimental in vivo trials and field studies, by establishing ex vivo and in vitro systems and by extensive molecular epidemiological studies.
Ryt-Hansen, P.; Pedersen, A.G.; Larsen, I.; Kristensen, C.S.; Krog, J.S.; Wacheck, S.; Larsen, L.E. 2020. Substantial Antigenic Drift in the Hemagglutinin Protein of Swine Influenza A Viruses. Viruses 12, 248. ; https://doi.org/10.3390/v12020248
Krog JS, Larsen LE and Breum SØ. 2019. Tracing Hepatitis E Virus in Pigs From Birth to Slaughter. Front. Vet. Sci. 6:50. https://doi.org/10.3389/fvets.2019.00050
Leyson C, Youk SS, Smith D, Dimitrov K, Lee DH, Larsen LE, Swayne DE, Pantin-Jackwood MJ. 2019. Pathogenicity and genomic changes of a 2016 European H5N8 highly pathogenic avian influenza virus (clade 220.127.116.11) in experimentally infected mallards and chickens. Virology.;537:172-185. https://doi.org/10.1016/j.virol.2019.08.020
Avian influenza: Diagnosis and molecular biological characterization
The project will increase our basic knowledge of the epidemiology of AIV in Danish conditions and will lead to the development of more refined diagnostic tests.
Grant: Veterinary contingency program. 2019-21. 2.5 million DKK.
FluZooMark: Viral and Host Factors of Zoonotic and Pandemic Influenza A Viruses
Researchers in virology and immunology in animals and humans will work together to characterize human and animal influenza viruses to find out why some influenza viruses can transmit from swine to humans. The project is expected to generate groundbreaking new tools for preventing future influenza pandemics in humans and provide knowledge that can be used in the future to design improved vaccines for humans and animals.
Funding: Novo Nordisk Foundation Challenge program 2019. 60 mill. DKK.
Porcine Reproductive and Respiratory Syndrom (PRRSV) and other important viral diseases in pigs in Denmark
The project comprises 3 subprojects:
1. Characterization of new recombinant PRRSV strain
2. The infectivity of PRRSV isolated from vaccinated ornamental station
3. Validation of saliva samples for monitoring boar stations
Funding: SEGES, Landbrug og Fødevare. 2020. 580,000 DKK.
Swine influenza viruses in Denmark: Prevalence and genetic and antigenic characterization
The overall purpose of the project is to investigate which influenza subtypes and variants that are circulating among Danish pigs and to identify disease causes in the pig population with a view to securing the strategic goal: to reduce antibiotic consumption in Danish pig herds.
Funding: CEVA Animal Health and the Danish Veterinary and Food Administration. 2020. DKK 883,000.
Tools for the control of viral infections in Danish pigs
The project covers three activities:
Activity 1. Consequences of mass vaccination against PRRSV in Danish swineherds.
Activity 2. Monitoring the diversity of Danish PRRSV strains.
Activity 3: Infection dynamics in boars after acute infection with PRRSV-1 (extra grant)
Funding: The Pig Levy Foundation. 2019-20. 996,000. DKK.
Selected ongoing projects:
FluZooMark - zoonotic aspects of swine influenza viruses - 2020-2025 - Novo Nordisk Foundation - 60 mill. DKK
PRRSV - sequencing, opathogenesis, vaccine strategies- 2020 - SEGES - 400,000 DKK
PRRSV - impact of mass vaccination - Svineafgiftsfonden/European PRRSV award - 450,000 DKK
Svine influenza virus - diversity, diagnostics, control - 2020 - 490,000 DKK
Avian influenza virus – pathogeneses and host range – DK-Vet Consortium – 2020-2022 – 3 mill. DKK
|George, Sophie Joanna||PhD fellow|
|Grønnegaard, Nina Dam||Laboratory technician||+45 353-28312|
|Kvisgaard, Lise Kirstine||Academic employee||+45 353-21617|
|Larsen, Lars Erik||Professor||+45 353-32329|
|Liang, Yuan||PhD fellow||+45 353-37865|
|Ryt-Hansen, Pia||Postdoc||+45 353-34530|
|Tran, Hue Thi Thanh||Laboratory technician||+45 353-22549|
|Welner, Simon||Postdoc||+45 353-20258|