Enzootic and zoonotic virus research: The Lars Erik Larsen group
The overarching goal of our work is to contribute to increased animal welfare and food safety in the Danish animal production. The core research areas are enzootic viral diseases in pigs and other production animals and zoonotic viruses in pigs and birds.
Influenza A virus (IAV) is an important pathogen in humans and animals and has zoonotic potential. Pigs are regarded as a melting pot for generation of new reassortant viruses potentially with zoonotic and pandemic capabilities.
“Thus, more knowledge on viral and host molecular determinants of host range restriction of new influenza A virus strains discovered in animals are urgently needed”, says research group leader Professor Lars Erik Larsen .
We want to extend our previous work to study the basic mechanism of host range determinants and virulence of influenza A viruses. We will establish in vitro and ex vivo cell and tissue cultures that allow the study of influenza virus/host interaction and infection dynamics and further develop the previous
Of scientifical highlights, my group was the first to demonstrate that the distribution of siliac acid receptors in pigs are similar to humans and that avian influenza virus primary replicates in type 2 pneumocytes of svine similar to humans.
During the last four years, we have performed a range of studies that emphatized that swIAV is a major veterinary pathogen in all age groups including piglets and that the virus persists in the herds and is subjected to significant antigenic and genetic evolution comparable to human influenza viruses. Furthermore, we have documented that the effect of vaccination and pre-existing immunity not only lack protective effects, but even may contribute to viral persistence. We want to focus on exploring the basic mechanism behind this by design of experimental in vivo trials and field studies, by establishing ex vivo and in vitro systems and by extensive molecular epidemiological studies.
Kvisgaard LK, Larsen LE, Kristensen CS, Paboeuf F, Renson P, Bourry O. 2021. Challenge of Naïve and Vaccinated Pigs with a Vaccine-Derived Recombinant Porcine Reproductive and Respiratory Syndrome Virus 1 Strain (Horsens Strain). Vaccines 9(5):417. 10.3390/vaccines9050417
Liang Y, Nissen JN, Krog JS, Breum SØ, Trebbien R, Larsen LE, Hjulsager CK. 2021. Novel Clade 18.104.22.168b Highly Pathogenic Avian Influenza A H5N8 and H5N5 Viruses in Denmark, 2020. Viruses 13(5):886. 10.3390/v13050886
Welner S, Ruggli N, Liniger M, Summerfield A, Larsen LE, Jungersen G. 2021. Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes. Vaccines 9(3):208.; https://doi.org/10.3390/vaccines9030208
Ryt-Hansen, P.; Pedersen, A.G.; Larsen, I.; Kristensen, C.S.; Krog, J.S.; Wacheck, S.; Larsen, L.E. 2020. Substantial Antigenic Drift in the Hemagglutinin Protein of Swine Influenza A Viruses. Viruses 12, 248. ; https://doi.org/10.3390/v12020248
Avian influenza: Diagnosis and molecular biological characterization
The project will increase our basic knowledge of the epidemiology of AIV in Danish conditions and will lead to the development of more refined diagnostic tests.
Grant: Veterinary contingency program. 2019-21. 2.5 million DKK.
The project aims to develop and implement knowledge-based counseling tools to control the most important virus infections in Danish pig production, specifically aimed at reducing piglet mortality.
Funding: Svineafgiftsfonden; 1,007,488 DKK. Period: 2021-2022.
FluZooMark: Viral and Host Factors of Zoonotic and Pandemic Influenza A Viruses
Researchers in virology and immunology in animals and humans will work together to characterize human and animal influenza viruses to find out why some influenza viruses can transmit from swine to humans. The project is expected to generate groundbreaking new tools for preventing future influenza pandemics in humans and provide knowledge that can be used in the future to design improved vaccines for humans and animals.
Funding: Novo Nordisk Foundation Challenge program 2019. 60 mill. DKK.
Researchers from Denmark, France, Germany, Italy, Spain and United Kingdom will perform a longitudinal that will provide essential knowledge for improved intervention and sustainable pig production, and will also be important for further development of more accurate diagnostic tools and relevant vaccines.
Funding: ICRAD; 1,353,000 DKK (1,375,00 EURO in total)
The overall purpose of the project is to investigate which influenza subtypes and variants that are circulating among Danish pigs and to identify disease causes in the pig population with a view to securing the strategic goal: to reduce antibiotic consumption in Danish pig herds.
Funding: CEVA Animal Health and the Danish Veterinary and Food Administration. 2021. DKK 1.054,000.
Selected ongoing projects:
FluZooMark - zoonotic aspects of swine influenza viruses - 2020-2025 - Novo Nordisk Foundation - 60 mill. DKK
PRRSV - sequencing, opathogenesis, vaccine strategies- 2020 - SEGES - 400,000 DKK
PRRSV - impact of mass vaccination - Svineafgiftsfonden/European PRRSV award - 450,000 DKK
Svine influenza virus - diversity, diagnostics, control - 2020 - 490,000 DKK
Avian influenza virus – pathogeneses and host range – DK-Vet Consortium – 2020-2022 – 3 mill. DKK
|George, Sophie Joanna||PhD Fellow|
|Goecke, Nicole Bakkegård||Postdoc||+4535327222|
|Grønnegaard, Nina Dam||Laboratory Technician||+4535328312|
|Kristensen, Charlotte||PhD Student|
|Kvisgaard, Lise Kirstine||Academic Research Staff||+4535321617|
|Larsen, Lars Erik||Professor||+4535332329|
|Liang, Yuan||PhD Fellow||+4535337865|
|Martiny, Karen Anna Handberg||Student||+4535322266|
|Tran, Hue Thi Thanh||Laboratory Technician||+4535322549|