Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: A new system using simple and aqueous-phase hybridization

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Standard

Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease : A new system using simple and aqueous-phase hybridization. / Alexandersen, S.; Forsyth, M. A.; Reid, S. M.; Belsham, G. J.

I: Journal of clinical microbiology, Bind 38, Nr. 12, 2000, s. 4604-4613.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Alexandersen, S, Forsyth, MA, Reid, SM & Belsham, GJ 2000, 'Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: A new system using simple and aqueous-phase hybridization', Journal of clinical microbiology, bind 38, nr. 12, s. 4604-4613. https://doi.org/10.1128/jcm.38.12.4604-4613.2000

APA

Alexandersen, S., Forsyth, M. A., Reid, S. M., & Belsham, G. J. (2000). Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: A new system using simple and aqueous-phase hybridization. Journal of clinical microbiology, 38(12), 4604-4613. https://doi.org/10.1128/jcm.38.12.4604-4613.2000

Vancouver

Alexandersen S, Forsyth MA, Reid SM, Belsham GJ. Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: A new system using simple and aqueous-phase hybridization. Journal of clinical microbiology. 2000;38(12):4604-4613. https://doi.org/10.1128/jcm.38.12.4604-4613.2000

Author

Alexandersen, S. ; Forsyth, M. A. ; Reid, S. M. ; Belsham, G. J. / Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease : A new system using simple and aqueous-phase hybridization. I: Journal of clinical microbiology. 2000 ; Bind 38, Nr. 12. s. 4604-4613.

Bibtex

@article{062f6c4e18694f4e849380f3e2ccae92,
title = "Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: A new system using simple and aqueous-phase hybridization",
abstract = "A reverse transcription-PCR (RT-PCR)-enzyme-linked immunosorbent assay system that detects a relatively conserved region within the RNA genome of all seven serotypes of foot-and-mouth disease virus (FMDV) has been developed. The high specificity of the assay is achieved by including a rapid hybridization step with a biotin-labeled internal oligonucleotide. The assay is highly sensitive, fast, and easy to perform. A similar assay, based on a highly variable region of the FMDV genome and employing a single asymmetric RT-PCR and multiple hybridization oligonucleotides, was developed to demonstrate the method's ability to type FMDV. Based on our theoretical and practical knowledge of the methodology, we predict that similar assays are applicable to diagnosis and strain differentiation in any system amenable to PCR amplification.",
author = "S. Alexandersen and Forsyth, {M. A.} and Reid, {S. M.} and Belsham, {G. J.}",
year = "2000",
doi = "10.1128/jcm.38.12.4604-4613.2000",
language = "English",
volume = "38",
pages = "4604--4613",
journal = "Journal of clinical microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "12",

}

RIS

TY - JOUR

T1 - Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease

T2 - A new system using simple and aqueous-phase hybridization

AU - Alexandersen, S.

AU - Forsyth, M. A.

AU - Reid, S. M.

AU - Belsham, G. J.

PY - 2000

Y1 - 2000

N2 - A reverse transcription-PCR (RT-PCR)-enzyme-linked immunosorbent assay system that detects a relatively conserved region within the RNA genome of all seven serotypes of foot-and-mouth disease virus (FMDV) has been developed. The high specificity of the assay is achieved by including a rapid hybridization step with a biotin-labeled internal oligonucleotide. The assay is highly sensitive, fast, and easy to perform. A similar assay, based on a highly variable region of the FMDV genome and employing a single asymmetric RT-PCR and multiple hybridization oligonucleotides, was developed to demonstrate the method's ability to type FMDV. Based on our theoretical and practical knowledge of the methodology, we predict that similar assays are applicable to diagnosis and strain differentiation in any system amenable to PCR amplification.

AB - A reverse transcription-PCR (RT-PCR)-enzyme-linked immunosorbent assay system that detects a relatively conserved region within the RNA genome of all seven serotypes of foot-and-mouth disease virus (FMDV) has been developed. The high specificity of the assay is achieved by including a rapid hybridization step with a biotin-labeled internal oligonucleotide. The assay is highly sensitive, fast, and easy to perform. A similar assay, based on a highly variable region of the FMDV genome and employing a single asymmetric RT-PCR and multiple hybridization oligonucleotides, was developed to demonstrate the method's ability to type FMDV. Based on our theoretical and practical knowledge of the methodology, we predict that similar assays are applicable to diagnosis and strain differentiation in any system amenable to PCR amplification.

UR - http://www.scopus.com/inward/record.url?scp=0034459012&partnerID=8YFLogxK

U2 - 10.1128/jcm.38.12.4604-4613.2000

DO - 10.1128/jcm.38.12.4604-4613.2000

M3 - Journal article

C2 - 11101603

AN - SCOPUS:0034459012

VL - 38

SP - 4604

EP - 4613

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

SN - 0095-1137

IS - 12

ER -

ID: 379027540