The mechanism of translation of cowpea mosaic virus middle component RNA: No evidence for internal initiation from experiments in an animal cell transient expression system

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Standard

The mechanism of translation of cowpea mosaic virus middle component RNA : No evidence for internal initiation from experiments in an animal cell transient expression system. / Belsham, G. J.; Lomonossoff, G. P.

I: Journal of General Virology, Bind 72, Nr. 12, 1991, s. 3109-3113.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Belsham, GJ & Lomonossoff, GP 1991, 'The mechanism of translation of cowpea mosaic virus middle component RNA: No evidence for internal initiation from experiments in an animal cell transient expression system', Journal of General Virology, bind 72, nr. 12, s. 3109-3113. https://doi.org/10.1099/0022-1317-72-12-3109

APA

Belsham, G. J., & Lomonossoff, G. P. (1991). The mechanism of translation of cowpea mosaic virus middle component RNA: No evidence for internal initiation from experiments in an animal cell transient expression system. Journal of General Virology, 72(12), 3109-3113. https://doi.org/10.1099/0022-1317-72-12-3109

Vancouver

Belsham GJ, Lomonossoff GP. The mechanism of translation of cowpea mosaic virus middle component RNA: No evidence for internal initiation from experiments in an animal cell transient expression system. Journal of General Virology. 1991;72(12):3109-3113. https://doi.org/10.1099/0022-1317-72-12-3109

Author

Belsham, G. J. ; Lomonossoff, G. P. / The mechanism of translation of cowpea mosaic virus middle component RNA : No evidence for internal initiation from experiments in an animal cell transient expression system. I: Journal of General Virology. 1991 ; Bind 72, Nr. 12. s. 3109-3113.

Bibtex

@article{b12e7dd66dd948eaac3c8b60c1a2d3ff,
title = "The mechanism of translation of cowpea mosaic virus middle component RNA: No evidence for internal initiation from experiments in an animal cell transient expression system",
abstract = "The possibility that internal initiation of translation is responsible for the synthesis of the middle component (M) RNA-encoded 95K protein of cowpea mosaic virus (CPMV) has been investigated by constructing plasmids in which the entire sequence of CPMV M RNA was cloned downstream of a chloramphenicol acetyltransferase gene. Expression of these plasmids in an animal cell expression system revealed that no synthesis of the proteins encoded by the downstream CPMV open reading frame takes place from RNA derived from these constructs under conditions where the internal ribosome entry site of foot-and-mouth disease virus is functional. The results indicate that internal initiation is not responsible for thc synthesis of the 95K protein in this system.",
author = "Belsham, {G. J.} and Lomonossoff, {G. P.}",
year = "1991",
doi = "10.1099/0022-1317-72-12-3109",
language = "English",
volume = "72",
pages = "3109--3113",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "12",

}

RIS

TY - JOUR

T1 - The mechanism of translation of cowpea mosaic virus middle component RNA

T2 - No evidence for internal initiation from experiments in an animal cell transient expression system

AU - Belsham, G. J.

AU - Lomonossoff, G. P.

PY - 1991

Y1 - 1991

N2 - The possibility that internal initiation of translation is responsible for the synthesis of the middle component (M) RNA-encoded 95K protein of cowpea mosaic virus (CPMV) has been investigated by constructing plasmids in which the entire sequence of CPMV M RNA was cloned downstream of a chloramphenicol acetyltransferase gene. Expression of these plasmids in an animal cell expression system revealed that no synthesis of the proteins encoded by the downstream CPMV open reading frame takes place from RNA derived from these constructs under conditions where the internal ribosome entry site of foot-and-mouth disease virus is functional. The results indicate that internal initiation is not responsible for thc synthesis of the 95K protein in this system.

AB - The possibility that internal initiation of translation is responsible for the synthesis of the middle component (M) RNA-encoded 95K protein of cowpea mosaic virus (CPMV) has been investigated by constructing plasmids in which the entire sequence of CPMV M RNA was cloned downstream of a chloramphenicol acetyltransferase gene. Expression of these plasmids in an animal cell expression system revealed that no synthesis of the proteins encoded by the downstream CPMV open reading frame takes place from RNA derived from these constructs under conditions where the internal ribosome entry site of foot-and-mouth disease virus is functional. The results indicate that internal initiation is not responsible for thc synthesis of the 95K protein in this system.

UR - http://www.scopus.com/inward/record.url?scp=0026317453&partnerID=8YFLogxK

U2 - 10.1099/0022-1317-72-12-3109

DO - 10.1099/0022-1317-72-12-3109

M3 - Journal article

C2 - 1765773

AN - SCOPUS:0026317453

VL - 72

SP - 3109

EP - 3113

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 12

ER -

ID: 381223085