A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

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A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. / Mohammadniaei, Mohsen; Zhang, Ming; Ashley, Jon; Christensen, Ulf Bech; Friis-Hansen, Lennart Jan; Gregersen, Rasmus; Lisby, Jan Gorm; Benfield, Thomas Lars; Nielsen, Finn Erland; Henning Rasmussen, Jens; Pedersen, Ellen Bøtker; Olinger, Anne Christine Rye; Kolding, Lærke Tørring; Naseri, Maryam; Zheng, Tao; Wang, Wentao; Gorodkin, Jan; Sun, Yi.

I: Nature Communications, Bind 12, 5089, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mohammadniaei, M, Zhang, M, Ashley, J, Christensen, UB, Friis-Hansen, LJ, Gregersen, R, Lisby, JG, Benfield, TL, Nielsen, FE, Henning Rasmussen, J, Pedersen, EB, Olinger, ACR, Kolding, LT, Naseri, M, Zheng, T, Wang, W, Gorodkin, J & Sun, Y 2021, 'A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA', Nature Communications, bind 12, 5089. https://doi.org/10.1038/s41467-021-25387-9

APA

Mohammadniaei, M., Zhang, M., Ashley, J., Christensen, U. B., Friis-Hansen, L. J., Gregersen, R., Lisby, J. G., Benfield, T. L., Nielsen, F. E., Henning Rasmussen, J., Pedersen, E. B., Olinger, A. C. R., Kolding, L. T., Naseri, M., Zheng, T., Wang, W., Gorodkin, J., & Sun, Y. (2021). A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications, 12, [5089]. https://doi.org/10.1038/s41467-021-25387-9

Vancouver

Mohammadniaei M, Zhang M, Ashley J, Christensen UB, Friis-Hansen LJ, Gregersen R o.a. A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications. 2021;12. 5089. https://doi.org/10.1038/s41467-021-25387-9

Author

Mohammadniaei, Mohsen ; Zhang, Ming ; Ashley, Jon ; Christensen, Ulf Bech ; Friis-Hansen, Lennart Jan ; Gregersen, Rasmus ; Lisby, Jan Gorm ; Benfield, Thomas Lars ; Nielsen, Finn Erland ; Henning Rasmussen, Jens ; Pedersen, Ellen Bøtker ; Olinger, Anne Christine Rye ; Kolding, Lærke Tørring ; Naseri, Maryam ; Zheng, Tao ; Wang, Wentao ; Gorodkin, Jan ; Sun, Yi. / A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. I: Nature Communications. 2021 ; Bind 12.

Bibtex

@article{a3f4c4b9e19d49c1bef8e9d5841ea94a,
title = "A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA",
abstract = "The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.",
author = "Mohsen Mohammadniaei and Ming Zhang and Jon Ashley and Christensen, {Ulf Bech} and Friis-Hansen, {Lennart Jan} and Rasmus Gregersen and Lisby, {Jan Gorm} and Benfield, {Thomas Lars} and Nielsen, {Finn Erland} and {Henning Rasmussen}, Jens and Pedersen, {Ellen B{\o}tker} and Olinger, {Anne Christine Rye} and Kolding, {L{\ae}rke T{\o}rring} and Maryam Naseri and Tao Zheng and Wentao Wang and Jan Gorodkin and Yi Sun",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
doi = "10.1038/s41467-021-25387-9",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

AU - Mohammadniaei, Mohsen

AU - Zhang, Ming

AU - Ashley, Jon

AU - Christensen, Ulf Bech

AU - Friis-Hansen, Lennart Jan

AU - Gregersen, Rasmus

AU - Lisby, Jan Gorm

AU - Benfield, Thomas Lars

AU - Nielsen, Finn Erland

AU - Henning Rasmussen, Jens

AU - Pedersen, Ellen Bøtker

AU - Olinger, Anne Christine Rye

AU - Kolding, Lærke Tørring

AU - Naseri, Maryam

AU - Zheng, Tao

AU - Wang, Wentao

AU - Gorodkin, Jan

AU - Sun, Yi

N1 - Publisher Copyright: © 2021, The Author(s).

PY - 2021

Y1 - 2021

N2 - The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

AB - The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

U2 - 10.1038/s41467-021-25387-9

DO - 10.1038/s41467-021-25387-9

M3 - Journal article

C2 - 34429424

AN - SCOPUS:85113296116

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 5089

ER -

ID: 279194747