Isolation and characterization of the gene encoding EF-1αO, an elongation factor 1-α expressed during early development of Xenopus laevis
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Isolation and characterization of the gene encoding EF-1αO, an elongation factor 1-α expressed during early development of Xenopus laevis. / Frydenberg, Jane; Poulsen, Knud; Petersen, Anne K.B.; Lund, Ann; Olesen, Ole F.
I: Gene, Bind 109, Nr. 2, 30.12.1991, s. 185-192.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Isolation and characterization of the gene encoding EF-1αO, an elongation factor 1-α expressed during early development of Xenopus laevis
AU - Frydenberg, Jane
AU - Poulsen, Knud
AU - Petersen, Anne K.B.
AU - Lund, Ann
AU - Olesen, Ole F.
PY - 1991/12/30
Y1 - 1991/12/30
N2 - In Xenopus laevis, the gene encoding the elongation factor 1-α variant EF-1αO, where O stands for oocyte, is expressed in oocytes and early embryos. A genomic library from X. laevis was screened with a cDNA probe coding for EF-1αO. Two recombinant phages were isolated, one of which carries an entire EF-1αO gene. This clone was characterized by restriction enzyme mapping and sequencing. Comparison of cDNA and genomic sequences revealed that EF-1αO gene, as all locations of the splice junctions are conserved between the two genes. The sequence immediately upstream from the transcription start point (tsp) contains a CCAAT box, but does not contain either a TATA box or a Sp1-binding site. Interestingly, this sequence has a sequence homologous to the negative regulatory element from the TFIIIA promoter. A region located about 400 bp upstream from the tsp contains an additional number of possible regulatory sequence elements. The first intron contains G + C-rich elements which exist both isolated and as part of longer inverted repeats. Furthermore, one octamer and four Sp1-binding sites are found in this intron. © 1991.
AB - In Xenopus laevis, the gene encoding the elongation factor 1-α variant EF-1αO, where O stands for oocyte, is expressed in oocytes and early embryos. A genomic library from X. laevis was screened with a cDNA probe coding for EF-1αO. Two recombinant phages were isolated, one of which carries an entire EF-1αO gene. This clone was characterized by restriction enzyme mapping and sequencing. Comparison of cDNA and genomic sequences revealed that EF-1αO gene, as all locations of the splice junctions are conserved between the two genes. The sequence immediately upstream from the transcription start point (tsp) contains a CCAAT box, but does not contain either a TATA box or a Sp1-binding site. Interestingly, this sequence has a sequence homologous to the negative regulatory element from the TFIIIA promoter. A region located about 400 bp upstream from the tsp contains an additional number of possible regulatory sequence elements. The first intron contains G + C-rich elements which exist both isolated and as part of longer inverted repeats. Furthermore, one octamer and four Sp1-binding sites are found in this intron. © 1991.
KW - Recombinant DNA
KW - differential expression
KW - exon
KW - intron
KW - promoter
KW - protein-binding sequences
UR - https://www.mendeley.com/catalogue/c31f904d-babb-36cc-9236-6647161eab8a/
U2 - 10.1016/0378-1119(91)90608-E
DO - 10.1016/0378-1119(91)90608-E
M3 - Journal article
C2 - 1765266
VL - 109
SP - 185
EP - 192
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -
ID: 252046072