Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors: Characterization and Applications for Immunoassays in Food Processing and Plant Breeding

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors : Characterization and Applications for Immunoassays in Food Processing and Plant Breeding. / FrØkiær, Hanne; HØrlyck, Lene; Barkholt, Vibeke; SØrensen, Susanne; s⊘rensen, S.

I: Food and Agricultural Immunology, Bind 6, Nr. 1, 1994, s. 63-72.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

FrØkiær, H, HØrlyck, L, Barkholt, V, SØrensen, S & s⊘rensen, S 1994, 'Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors: Characterization and Applications for Immunoassays in Food Processing and Plant Breeding', Food and Agricultural Immunology, bind 6, nr. 1, s. 63-72. https://doi.org/10.1080/09540109409354814

APA

FrØkiær, H., HØrlyck, L., Barkholt, V., SØrensen, S., & s⊘rensen, S. (1994). Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors: Characterization and Applications for Immunoassays in Food Processing and Plant Breeding. Food and Agricultural Immunology, 6(1), 63-72. https://doi.org/10.1080/09540109409354814

Vancouver

FrØkiær H, HØrlyck L, Barkholt V, SØrensen S, s⊘rensen S. Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors: Characterization and Applications for Immunoassays in Food Processing and Plant Breeding. Food and Agricultural Immunology. 1994;6(1):63-72. https://doi.org/10.1080/09540109409354814

Author

FrØkiær, Hanne ; HØrlyck, Lene ; Barkholt, Vibeke ; SØrensen, Susanne ; s⊘rensen, S. / Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors : Characterization and Applications for Immunoassays in Food Processing and Plant Breeding. I: Food and Agricultural Immunology. 1994 ; Bind 6, Nr. 1. s. 63-72.

Bibtex

@article{a0e28119811e4a258df866f884c4a27f,
title = "Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors: Characterization and Applications for Immunoassays in Food Processing and Plant Breeding",
abstract = "Monoclonal antibodies produced against protein-type inhibitors from pea or soybean were used for investigations of the chemical and antigenic properties of trypsin and chymotrypsin inhibitors from pea. Cross-reactivity studies revealed only the existence of Bowman-Birk-type inhibitors in pea. The inhibitors could be divided into at least two groups of iso-inhibitors based on their characteristics in binding to different monoclonal antibodies produced against pea trypsin inhibitors or Bowman-Birk inhibitor from soybean. The inhibitor contents in a series of pea extracts were measured in an ELISA-based system using the different antibodies. Comparison with the inhibitor activity measured by traditional enzymatic analysis performed as microassay showed that only the inhibitor content of one of the two inhibitor groups correlated strongly with the trypsin inhibitor activity. The immunoassay was also shown to be suitable for measurement of inhibitor content after heat treatment as the results suggested that the two inhibitor groups contained equally heat resistant inhibitors.",
keywords = "inactivation, Pea protein type proteinase inhibitors monoclonal antibodies ELISA plant breeding",
author = "Hanne Fr{\O}ki{\ae}r and Lene H{\O}rlyck and Vibeke Barkholt and Susanne S{\O}rensen and S. s⊘rensen",
year = "1994",
doi = "10.1080/09540109409354814",
language = "English",
volume = "6",
pages = "63--72",
journal = "Food and Agricultural Immunology",
issn = "0954-0105",
publisher = "Taylor & Francis",
number = "1",

}

RIS

TY - JOUR

T1 - Monoclonal Antibodies against Soybean and Pea Proteinase Inhibitors

T2 - Characterization and Applications for Immunoassays in Food Processing and Plant Breeding

AU - FrØkiær, Hanne

AU - HØrlyck, Lene

AU - Barkholt, Vibeke

AU - SØrensen, Susanne

AU - s⊘rensen, S.

PY - 1994

Y1 - 1994

N2 - Monoclonal antibodies produced against protein-type inhibitors from pea or soybean were used for investigations of the chemical and antigenic properties of trypsin and chymotrypsin inhibitors from pea. Cross-reactivity studies revealed only the existence of Bowman-Birk-type inhibitors in pea. The inhibitors could be divided into at least two groups of iso-inhibitors based on their characteristics in binding to different monoclonal antibodies produced against pea trypsin inhibitors or Bowman-Birk inhibitor from soybean. The inhibitor contents in a series of pea extracts were measured in an ELISA-based system using the different antibodies. Comparison with the inhibitor activity measured by traditional enzymatic analysis performed as microassay showed that only the inhibitor content of one of the two inhibitor groups correlated strongly with the trypsin inhibitor activity. The immunoassay was also shown to be suitable for measurement of inhibitor content after heat treatment as the results suggested that the two inhibitor groups contained equally heat resistant inhibitors.

AB - Monoclonal antibodies produced against protein-type inhibitors from pea or soybean were used for investigations of the chemical and antigenic properties of trypsin and chymotrypsin inhibitors from pea. Cross-reactivity studies revealed only the existence of Bowman-Birk-type inhibitors in pea. The inhibitors could be divided into at least two groups of iso-inhibitors based on their characteristics in binding to different monoclonal antibodies produced against pea trypsin inhibitors or Bowman-Birk inhibitor from soybean. The inhibitor contents in a series of pea extracts were measured in an ELISA-based system using the different antibodies. Comparison with the inhibitor activity measured by traditional enzymatic analysis performed as microassay showed that only the inhibitor content of one of the two inhibitor groups correlated strongly with the trypsin inhibitor activity. The immunoassay was also shown to be suitable for measurement of inhibitor content after heat treatment as the results suggested that the two inhibitor groups contained equally heat resistant inhibitors.

KW - inactivation

KW - Pea protein type proteinase inhibitors monoclonal antibodies ELISA plant breeding

UR - http://www.scopus.com/inward/record.url?scp=0001879699&partnerID=8YFLogxK

U2 - 10.1080/09540109409354814

DO - 10.1080/09540109409354814

M3 - Journal article

AN - SCOPUS:0001879699

VL - 6

SP - 63

EP - 72

JO - Food and Agricultural Immunology

JF - Food and Agricultural Immunology

SN - 0954-0105

IS - 1

ER -

ID: 331795246