Purification of egg‐white allergens
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Purification of egg‐white allergens. / Ebbehø, K.; Dahl, A. M.; Frøkiær, H.; Nørgaard, A.; Poulsen, L. K.; Barkholt, V.
I: Allergy, Bind 50, Nr. 2, 02.1995, s. 133-141.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Purification of egg‐white allergens
AU - Ebbehø, K.
AU - Dahl, A. M.
AU - Frøkiær, H.
AU - Nørgaard, A.
AU - Poulsen, L. K.
AU - Barkholt, V.
PY - 1995/2
Y1 - 1995/2
N2 - Purification procedures for the four egg‐white proteins ovomucoid, ovotransferrin, ovalbumin, and lysozyme are presented with reference to mechanistic studies at epitope levels of allergic reactions to these proteins. The applied procedures resulted in four preparations containing less than 0.1% contaminating proteins each. The purified protein preparations were characterized by SDS—PAGE and by crossed Immunoelectrophoresis with polyclonal antibodies raised against an egg‐white extract or the purified proteins. The necessity of these well‐characterized proteins in studies on allergic reactions was shown by testing human sera in immunoblots of lysozyme, and by immunoblots of ovomucoid probing with antibodies against the proteins. 1995 Munksgaard
AB - Purification procedures for the four egg‐white proteins ovomucoid, ovotransferrin, ovalbumin, and lysozyme are presented with reference to mechanistic studies at epitope levels of allergic reactions to these proteins. The applied procedures resulted in four preparations containing less than 0.1% contaminating proteins each. The purified protein preparations were characterized by SDS—PAGE and by crossed Immunoelectrophoresis with polyclonal antibodies raised against an egg‐white extract or the purified proteins. The necessity of these well‐characterized proteins in studies on allergic reactions was shown by testing human sera in immunoblots of lysozyme, and by immunoblots of ovomucoid probing with antibodies against the proteins. 1995 Munksgaard
KW - egg protein immunology
KW - food allergens
KW - food allergy
KW - food hypersensitivity
KW - hen's egg white
KW - lysozyme
KW - ovalbumin
KW - ovomucoid
KW - ovotransferrin
UR - http://www.scopus.com/inward/record.url?scp=0028953427&partnerID=8YFLogxK
U2 - 10.1111/j.1398-9995.1995.tb05069.x
DO - 10.1111/j.1398-9995.1995.tb05069.x
M3 - Journal article
C2 - 7604935
AN - SCOPUS:0028953427
VL - 50
SP - 133
EP - 141
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 2
ER -
ID: 328568419