Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences

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Standard

Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences. / McInerney, Gerald M.; King, Andrew M.Q.; Ross-Smith, Natalie; Belsham, Graham J.

I: Journal of General Virology, Bind 81, Nr. 7, 2000, s. 1699-1702.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

McInerney, GM, King, AMQ, Ross-Smith, N & Belsham, GJ 2000, 'Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences', Journal of General Virology, bind 81, nr. 7, s. 1699-1702. https://doi.org/10.1099/0022-1317-81-7-1699

APA

McInerney, G. M., King, A. M. Q., Ross-Smith, N., & Belsham, G. J. (2000). Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences. Journal of General Virology, 81(7), 1699-1702. https://doi.org/10.1099/0022-1317-81-7-1699

Vancouver

McInerney GM, King AMQ, Ross-Smith N, Belsham GJ. Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences. Journal of General Virology. 2000;81(7):1699-1702. https://doi.org/10.1099/0022-1317-81-7-1699

Author

McInerney, Gerald M. ; King, Andrew M.Q. ; Ross-Smith, Natalie ; Belsham, Graham J. / Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences. I: Journal of General Virology. 2000 ; Bind 81, Nr. 7. s. 1699-1702.

Bibtex

@article{2e4d784b983245e984322a2b6ea88bbb,
title = "Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences",
abstract = "RNA transcripts were prepared from plasmids encoding an infectious cDNA of foot-and-mouth disease virus (FMDV) or derivatives in which the leader (Lab and Lb) and capsid protein coding sequences were deleted or replaced by sequences encoding chloramphenicol acetyltransferase (CAT). The transcripts were electroporated into BHK cells and the expression of CAT and the FMDV 3C protease was monitored. Detection of CAT and 3C was dependent on the ability of the transcript to replicate. All of the Lb coding sequence and 94% of P1 (the capsid protein precursor) coding sequence could be deleted without any apparent effect on the ability of the RNA to replicate. Thus, no cis-acting replication element is present within this region of the FMDV genome. Trans-encapsidation of these FMDV replicons was very inefficient, which may explain the lack of production of defective-interfering particles in FMDV-infected cells.",
author = "McInerney, {Gerald M.} and King, {Andrew M.Q.} and Natalie Ross-Smith and Belsham, {Graham J.}",
year = "2000",
doi = "10.1099/0022-1317-81-7-1699",
language = "English",
volume = "81",
pages = "1699--1702",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences

AU - McInerney, Gerald M.

AU - King, Andrew M.Q.

AU - Ross-Smith, Natalie

AU - Belsham, Graham J.

PY - 2000

Y1 - 2000

N2 - RNA transcripts were prepared from plasmids encoding an infectious cDNA of foot-and-mouth disease virus (FMDV) or derivatives in which the leader (Lab and Lb) and capsid protein coding sequences were deleted or replaced by sequences encoding chloramphenicol acetyltransferase (CAT). The transcripts were electroporated into BHK cells and the expression of CAT and the FMDV 3C protease was monitored. Detection of CAT and 3C was dependent on the ability of the transcript to replicate. All of the Lb coding sequence and 94% of P1 (the capsid protein precursor) coding sequence could be deleted without any apparent effect on the ability of the RNA to replicate. Thus, no cis-acting replication element is present within this region of the FMDV genome. Trans-encapsidation of these FMDV replicons was very inefficient, which may explain the lack of production of defective-interfering particles in FMDV-infected cells.

AB - RNA transcripts were prepared from plasmids encoding an infectious cDNA of foot-and-mouth disease virus (FMDV) or derivatives in which the leader (Lab and Lb) and capsid protein coding sequences were deleted or replaced by sequences encoding chloramphenicol acetyltransferase (CAT). The transcripts were electroporated into BHK cells and the expression of CAT and the FMDV 3C protease was monitored. Detection of CAT and 3C was dependent on the ability of the transcript to replicate. All of the Lb coding sequence and 94% of P1 (the capsid protein precursor) coding sequence could be deleted without any apparent effect on the ability of the RNA to replicate. Thus, no cis-acting replication element is present within this region of the FMDV genome. Trans-encapsidation of these FMDV replicons was very inefficient, which may explain the lack of production of defective-interfering particles in FMDV-infected cells.

UR - http://www.scopus.com/inward/record.url?scp=0033947728&partnerID=8YFLogxK

U2 - 10.1099/0022-1317-81-7-1699

DO - 10.1099/0022-1317-81-7-1699

M3 - Journal article

C2 - 10859374

AN - SCOPUS:0033947728

VL - 81

SP - 1699

EP - 1702

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 7

ER -

ID: 379027849