Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso

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Standard

Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso. / Rasmussen, Henrik N.; Rasmussen, Ole F.; Andersen, Jens K.; Olsen, John E.

I: Molecular and Cellular Probes, Bind 8, Nr. 2, 04.1994, s. 99-108.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rasmussen, HN, Rasmussen, OF, Andersen, JK & Olsen, JE 1994, 'Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso', Molecular and Cellular Probes, bind 8, nr. 2, s. 99-108. https://doi.org/10.1006/mcpr.1994.1014

APA

Rasmussen, H. N., Rasmussen, O. F., Andersen, J. K., & Olsen, J. E. (1994). Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso. Molecular and Cellular Probes, 8(2), 99-108. https://doi.org/10.1006/mcpr.1994.1014

Vancouver

Rasmussen HN, Rasmussen OF, Andersen JK, Olsen JE. Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso. Molecular and Cellular Probes. 1994 apr.;8(2):99-108. https://doi.org/10.1006/mcpr.1994.1014

Author

Rasmussen, Henrik N. ; Rasmussen, Ole F. ; Andersen, Jens K. ; Olsen, John E. / Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso. I: Molecular and Cellular Probes. 1994 ; Bind 8, Nr. 2. s. 99-108.

Bibtex

@article{85d6e084bab845fa97aa11a8ec199cf4,
title = "Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso",
abstract = "A pair of polymerase chain reaction (PCR) primers, YC1 and YC2, selected from the sequence of the invasin locus (inv) of Y. enterocolitica, has been evaluated for specific detection of pathogenic Y. enterocolitica by PCR. The primers were hybridized at high stringency conditions to DNA from 65 pathogenic Y. enterocolitica, 16 non-pathogenic Y. enterocolitica, 18 other Yersinia and 124 non-Yersinia strains. YC2 hybridized to the pathogenic Y. enterocolitica only, while YC1 hybridized weakly to nine non-Yersinia as well. In a PCR with annealing at 64°C all Y. enterocolitica, pathogenic and non-pathogenic, were positive. However, DNA from 60 non-Y. enterocolitica was amplified. With annealing at 72°C, 10 non-pathogenic Y. enterocolitica and 41 non-Y. enterocolitica were positive. When a two-step PCR assay with annealing at 72°C, hot-start and 1% dimethylsulphoxide (DMSO) were used, only DNA from the pathogenic Y. enterocolitica were amplified. The limit of detection was shown for four different strains to be less than 10 cells per PCR tube.",
keywords = "DMSO, Hot-start, Invasin, Polymerase chain reaction, Yersinia enterocolitica",
author = "Rasmussen, {Henrik N.} and Rasmussen, {Ole F.} and Andersen, {Jens K.} and Olsen, {John E.}",
year = "1994",
month = apr,
doi = "10.1006/mcpr.1994.1014",
language = "English",
volume = "8",
pages = "99--108",
journal = "Molecular and Cellular Probes",
issn = "0890-8508",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Specific detection of pathogenic yersinia enterocolitica by two-step pcr using hot-start and dmso

AU - Rasmussen, Henrik N.

AU - Rasmussen, Ole F.

AU - Andersen, Jens K.

AU - Olsen, John E.

PY - 1994/4

Y1 - 1994/4

N2 - A pair of polymerase chain reaction (PCR) primers, YC1 and YC2, selected from the sequence of the invasin locus (inv) of Y. enterocolitica, has been evaluated for specific detection of pathogenic Y. enterocolitica by PCR. The primers were hybridized at high stringency conditions to DNA from 65 pathogenic Y. enterocolitica, 16 non-pathogenic Y. enterocolitica, 18 other Yersinia and 124 non-Yersinia strains. YC2 hybridized to the pathogenic Y. enterocolitica only, while YC1 hybridized weakly to nine non-Yersinia as well. In a PCR with annealing at 64°C all Y. enterocolitica, pathogenic and non-pathogenic, were positive. However, DNA from 60 non-Y. enterocolitica was amplified. With annealing at 72°C, 10 non-pathogenic Y. enterocolitica and 41 non-Y. enterocolitica were positive. When a two-step PCR assay with annealing at 72°C, hot-start and 1% dimethylsulphoxide (DMSO) were used, only DNA from the pathogenic Y. enterocolitica were amplified. The limit of detection was shown for four different strains to be less than 10 cells per PCR tube.

AB - A pair of polymerase chain reaction (PCR) primers, YC1 and YC2, selected from the sequence of the invasin locus (inv) of Y. enterocolitica, has been evaluated for specific detection of pathogenic Y. enterocolitica by PCR. The primers were hybridized at high stringency conditions to DNA from 65 pathogenic Y. enterocolitica, 16 non-pathogenic Y. enterocolitica, 18 other Yersinia and 124 non-Yersinia strains. YC2 hybridized to the pathogenic Y. enterocolitica only, while YC1 hybridized weakly to nine non-Yersinia as well. In a PCR with annealing at 64°C all Y. enterocolitica, pathogenic and non-pathogenic, were positive. However, DNA from 60 non-Y. enterocolitica was amplified. With annealing at 72°C, 10 non-pathogenic Y. enterocolitica and 41 non-Y. enterocolitica were positive. When a two-step PCR assay with annealing at 72°C, hot-start and 1% dimethylsulphoxide (DMSO) were used, only DNA from the pathogenic Y. enterocolitica were amplified. The limit of detection was shown for four different strains to be less than 10 cells per PCR tube.

KW - DMSO

KW - Hot-start

KW - Invasin

KW - Polymerase chain reaction

KW - Yersinia enterocolitica

U2 - 10.1006/mcpr.1994.1014

DO - 10.1006/mcpr.1994.1014

M3 - Journal article

C2 - 7935518

AN - SCOPUS:0028283843

VL - 8

SP - 99

EP - 108

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

SN - 0890-8508

IS - 2

ER -

ID: 251185880