Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures

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Standard

Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures. / Olsen, J. E.; Brown, D. J.; Baggesen, Dorte L.; Bisgaard, M.

I: Journal of Applied Bacteriology, Bind 77, Nr. 2, 08.1994, s. 155-159.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Olsen, JE, Brown, DJ, Baggesen, DL & Bisgaard, M 1994, 'Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures', Journal of Applied Bacteriology, bind 77, nr. 2, s. 155-159. https://doi.org/10.1111/j.1365-2672.1994.tb03059.x

APA

Olsen, J. E., Brown, D. J., Baggesen, D. L., & Bisgaard, M. (1994). Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures. Journal of Applied Bacteriology, 77(2), 155-159. https://doi.org/10.1111/j.1365-2672.1994.tb03059.x

Vancouver

Olsen JE, Brown DJ, Baggesen DL, Bisgaard M. Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures. Journal of Applied Bacteriology. 1994 aug.;77(2):155-159. https://doi.org/10.1111/j.1365-2672.1994.tb03059.x

Author

Olsen, J. E. ; Brown, D. J. ; Baggesen, Dorte L. ; Bisgaard, M. / Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures. I: Journal of Applied Bacteriology. 1994 ; Bind 77, Nr. 2. s. 155-159.

Bibtex

@article{85f02f31a9094ccda9385b3d5257b15d,
title = "Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures",
abstract = "Four strains of Salmonella berta and one of Salm. enteritidis were stored as stab cultures in sugar‐free agar at 5°, 22° and 30°C and in 15% glycerol at—80°C. The stability of the plasmid profiles in each of the strains was monitored over a period of 2·5 years. Plasmid profiles were stable in all strains stored at—80°C, and only six of 450 colonies examined from strains kept in sugar‐free agar at 5°C had lost plasmid molecules. Seventy of 440 colonies from stab cultures that were kept at 22°C, and 71 of 440 colonies at 30°C showed changed plasmid profiles. The total number of plasmids lost increased with time, and occasionally, more than one plasmid molecule was lost in the same strain. The virulence associated plasmid of Salm. enteritidis was remarkably stable as it was maintained in all colonies examined at all temperatures investigated. Likewise, no change in SmaI restriction profile was observed in this plasmid molecule at any temperature.",
author = "Olsen, {J. E.} and Brown, {D. J.} and Baggesen, {Dorte L.} and M. Bisgaard",
year = "1994",
month = aug,
doi = "10.1111/j.1365-2672.1994.tb03059.x",
language = "English",
volume = "77",
pages = "155--159",
journal = "Journal of Applied Bacteriology",
issn = "0021-8847",
publisher = "The Society for Applied Bacteriology",
number = "2",

}

RIS

TY - JOUR

T1 - Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures

AU - Olsen, J. E.

AU - Brown, D. J.

AU - Baggesen, Dorte L.

AU - Bisgaard, M.

PY - 1994/8

Y1 - 1994/8

N2 - Four strains of Salmonella berta and one of Salm. enteritidis were stored as stab cultures in sugar‐free agar at 5°, 22° and 30°C and in 15% glycerol at—80°C. The stability of the plasmid profiles in each of the strains was monitored over a period of 2·5 years. Plasmid profiles were stable in all strains stored at—80°C, and only six of 450 colonies examined from strains kept in sugar‐free agar at 5°C had lost plasmid molecules. Seventy of 440 colonies from stab cultures that were kept at 22°C, and 71 of 440 colonies at 30°C showed changed plasmid profiles. The total number of plasmids lost increased with time, and occasionally, more than one plasmid molecule was lost in the same strain. The virulence associated plasmid of Salm. enteritidis was remarkably stable as it was maintained in all colonies examined at all temperatures investigated. Likewise, no change in SmaI restriction profile was observed in this plasmid molecule at any temperature.

AB - Four strains of Salmonella berta and one of Salm. enteritidis were stored as stab cultures in sugar‐free agar at 5°, 22° and 30°C and in 15% glycerol at—80°C. The stability of the plasmid profiles in each of the strains was monitored over a period of 2·5 years. Plasmid profiles were stable in all strains stored at—80°C, and only six of 450 colonies examined from strains kept in sugar‐free agar at 5°C had lost plasmid molecules. Seventy of 440 colonies from stab cultures that were kept at 22°C, and 71 of 440 colonies at 30°C showed changed plasmid profiles. The total number of plasmids lost increased with time, and occasionally, more than one plasmid molecule was lost in the same strain. The virulence associated plasmid of Salm. enteritidis was remarkably stable as it was maintained in all colonies examined at all temperatures investigated. Likewise, no change in SmaI restriction profile was observed in this plasmid molecule at any temperature.

U2 - 10.1111/j.1365-2672.1994.tb03059.x

DO - 10.1111/j.1365-2672.1994.tb03059.x

M3 - Journal article

C2 - 7961188

AN - SCOPUS:0027994222

VL - 77

SP - 155

EP - 159

JO - Journal of Applied Bacteriology

JF - Journal of Applied Bacteriology

SN - 0021-8847

IS - 2

ER -

ID: 251186531