The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates: Implications for the baculovirus expression system

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates : Implications for the baculovirus expression system. / Royall, Elizabeth; Woolaway, Kathryn E.; Schacherl, Jens; Kubick, Stefan; Belsham, Graham J.; Roberts, Lisa O.

I: Journal of General Virology, Bind 85, Nr. 6, 06.2004, s. 1565-1569.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Royall, E, Woolaway, KE, Schacherl, J, Kubick, S, Belsham, GJ & Roberts, LO 2004, 'The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates: Implications for the baculovirus expression system', Journal of General Virology, bind 85, nr. 6, s. 1565-1569. https://doi.org/10.1099/vir.0.79992-0

APA

Royall, E., Woolaway, K. E., Schacherl, J., Kubick, S., Belsham, G. J., & Roberts, L. O. (2004). The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates: Implications for the baculovirus expression system. Journal of General Virology, 85(6), 1565-1569. https://doi.org/10.1099/vir.0.79992-0

Vancouver

Royall E, Woolaway KE, Schacherl J, Kubick S, Belsham GJ, Roberts LO. The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates: Implications for the baculovirus expression system. Journal of General Virology. 2004 jun.;85(6):1565-1569. https://doi.org/10.1099/vir.0.79992-0

Author

Royall, Elizabeth ; Woolaway, Kathryn E. ; Schacherl, Jens ; Kubick, Stefan ; Belsham, Graham J. ; Roberts, Lisa O. / The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates : Implications for the baculovirus expression system. I: Journal of General Virology. 2004 ; Bind 85, Nr. 6. s. 1565-1569.

Bibtex

@article{d16771b728554d9aa7d28906af3134d0,
title = "The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates: Implications for the baculovirus expression system",
abstract = "Cap-independent internal initiation of translation occurs on a number of viral and cellular mRNAs and is directed by internal ribosome entry site (IRES) elements. Rhopalosiphum padi virus (RhPV) is a member of the Dicistroviridae. These viruses have single-stranded, positive-sense RNA genomes that contain two open reading frames, both preceded by IRES elements. Previously, the activity of the RhPV 5′ UTR IRES has been demonstrated in mammalian, Drosophila and wheat germ in vitro translation systems. It is now shown that this IRES also functions within Spodoptera frugiperda (Sf21) cells which are widely used in the baculovirus expression system, and in a novel Sf21 cell-based lysate system. Inclusion of the RhPV IRES in a dicistronic reporter mRNA transcript increased translation of the second cistron 23-fold within Sf21 cells. In contrast, the encephalomyocarditis virus IRES was inactive in both systems. The RhPV IRES therefore has the potential to be utilized in insect cell expression systems.",
author = "Elizabeth Royall and Woolaway, {Kathryn E.} and Jens Schacherl and Stefan Kubick and Belsham, {Graham J.} and Roberts, {Lisa O.}",
year = "2004",
month = jun,
doi = "10.1099/vir.0.79992-0",
language = "English",
volume = "85",
pages = "1565--1569",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - The Rhopalosiphum padi virus 5′ internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates

T2 - Implications for the baculovirus expression system

AU - Royall, Elizabeth

AU - Woolaway, Kathryn E.

AU - Schacherl, Jens

AU - Kubick, Stefan

AU - Belsham, Graham J.

AU - Roberts, Lisa O.

PY - 2004/6

Y1 - 2004/6

N2 - Cap-independent internal initiation of translation occurs on a number of viral and cellular mRNAs and is directed by internal ribosome entry site (IRES) elements. Rhopalosiphum padi virus (RhPV) is a member of the Dicistroviridae. These viruses have single-stranded, positive-sense RNA genomes that contain two open reading frames, both preceded by IRES elements. Previously, the activity of the RhPV 5′ UTR IRES has been demonstrated in mammalian, Drosophila and wheat germ in vitro translation systems. It is now shown that this IRES also functions within Spodoptera frugiperda (Sf21) cells which are widely used in the baculovirus expression system, and in a novel Sf21 cell-based lysate system. Inclusion of the RhPV IRES in a dicistronic reporter mRNA transcript increased translation of the second cistron 23-fold within Sf21 cells. In contrast, the encephalomyocarditis virus IRES was inactive in both systems. The RhPV IRES therefore has the potential to be utilized in insect cell expression systems.

AB - Cap-independent internal initiation of translation occurs on a number of viral and cellular mRNAs and is directed by internal ribosome entry site (IRES) elements. Rhopalosiphum padi virus (RhPV) is a member of the Dicistroviridae. These viruses have single-stranded, positive-sense RNA genomes that contain two open reading frames, both preceded by IRES elements. Previously, the activity of the RhPV 5′ UTR IRES has been demonstrated in mammalian, Drosophila and wheat germ in vitro translation systems. It is now shown that this IRES also functions within Spodoptera frugiperda (Sf21) cells which are widely used in the baculovirus expression system, and in a novel Sf21 cell-based lysate system. Inclusion of the RhPV IRES in a dicistronic reporter mRNA transcript increased translation of the second cistron 23-fold within Sf21 cells. In contrast, the encephalomyocarditis virus IRES was inactive in both systems. The RhPV IRES therefore has the potential to be utilized in insect cell expression systems.

UR - http://www.scopus.com/inward/record.url?scp=2942635636&partnerID=8YFLogxK

U2 - 10.1099/vir.0.79992-0

DO - 10.1099/vir.0.79992-0

M3 - Journal article

C2 - 15166440

AN - SCOPUS:2942635636

VL - 85

SP - 1565

EP - 1569

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 6

ER -

ID: 379026304