Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan

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Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan. / Kaku, Yoshihiro; Chard, Louisa S.; Inoue, Toru; Belsham, Graham J.

I: Journal of Virology, Bind 76, Nr. 22, 11.2002, s. 11721-11728.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kaku, Y, Chard, LS, Inoue, T & Belsham, GJ 2002, 'Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan', Journal of Virology, bind 76, nr. 22, s. 11721-11728. https://doi.org/10.1128/JVI.76.22.11721-11728.2002

APA

Kaku, Y., Chard, L. S., Inoue, T., & Belsham, G. J. (2002). Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan. Journal of Virology, 76(22), 11721-11728. https://doi.org/10.1128/JVI.76.22.11721-11728.2002

Vancouver

Kaku Y, Chard LS, Inoue T, Belsham GJ. Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan. Journal of Virology. 2002 nov.;76(22):11721-11728. https://doi.org/10.1128/JVI.76.22.11721-11728.2002

Author

Kaku, Yoshihiro ; Chard, Louisa S. ; Inoue, Toru ; Belsham, Graham J. / Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan. I: Journal of Virology. 2002 ; Bind 76, Nr. 22. s. 11721-11728.

Bibtex

@article{e0bfbeb8bf8e4d8f9547f1c5793c765d,
title = "Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan",
abstract = "The teschoviruses constitute a recently defined picornavirus genus. Most of the genome sequence of the porcine teschovirus-1 (PTV) Talfan and several other strains is known. We now demonstrate that initiation of protein synthesis occurs at nucleotide (nt) 412 on the PTV Talfan RNA and that nt 1 to 405 contains an internal ribosome entry site (IRES) that functions efficiently in vitro and within mammalian cells. In comparison with other picornavirus IRES elements, the PTV IRES is relatively short and lacks a significant polypyrimidine tract near the 3′ end. Expression of an enterovirus 2A protease, which induces cleavage of eIF4G within the translation initiation complex eIF4F, has little effect on the PTV IRES activity within BHK cells. The PTV IRES has a unique set of properties and represents a new class of picornavirus IRES element.",
author = "Yoshihiro Kaku and Chard, {Louisa S.} and Toru Inoue and Belsham, {Graham J.}",
year = "2002",
month = nov,
doi = "10.1128/JVI.76.22.11721-11728.2002",
language = "English",
volume = "76",
pages = "11721--11728",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "22",

}

RIS

TY - JOUR

T1 - Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan

AU - Kaku, Yoshihiro

AU - Chard, Louisa S.

AU - Inoue, Toru

AU - Belsham, Graham J.

PY - 2002/11

Y1 - 2002/11

N2 - The teschoviruses constitute a recently defined picornavirus genus. Most of the genome sequence of the porcine teschovirus-1 (PTV) Talfan and several other strains is known. We now demonstrate that initiation of protein synthesis occurs at nucleotide (nt) 412 on the PTV Talfan RNA and that nt 1 to 405 contains an internal ribosome entry site (IRES) that functions efficiently in vitro and within mammalian cells. In comparison with other picornavirus IRES elements, the PTV IRES is relatively short and lacks a significant polypyrimidine tract near the 3′ end. Expression of an enterovirus 2A protease, which induces cleavage of eIF4G within the translation initiation complex eIF4F, has little effect on the PTV IRES activity within BHK cells. The PTV IRES has a unique set of properties and represents a new class of picornavirus IRES element.

AB - The teschoviruses constitute a recently defined picornavirus genus. Most of the genome sequence of the porcine teschovirus-1 (PTV) Talfan and several other strains is known. We now demonstrate that initiation of protein synthesis occurs at nucleotide (nt) 412 on the PTV Talfan RNA and that nt 1 to 405 contains an internal ribosome entry site (IRES) that functions efficiently in vitro and within mammalian cells. In comparison with other picornavirus IRES elements, the PTV IRES is relatively short and lacks a significant polypyrimidine tract near the 3′ end. Expression of an enterovirus 2A protease, which induces cleavage of eIF4G within the translation initiation complex eIF4F, has little effect on the PTV IRES activity within BHK cells. The PTV IRES has a unique set of properties and represents a new class of picornavirus IRES element.

UR - http://www.scopus.com/inward/record.url?scp=0036827978&partnerID=8YFLogxK

U2 - 10.1128/JVI.76.22.11721-11728.2002

DO - 10.1128/JVI.76.22.11721-11728.2002

M3 - Journal article

C2 - 12388732

AN - SCOPUS:0036827978

VL - 76

SP - 11721

EP - 11728

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 22

ER -

ID: 379026758