TY - JOUR

T1 - 3D-Printed Fluorescence Hyperspectral Lidar for Monitoring Tagged Insects

AU - Manefjord, Hampus

AU - Muller, Lauro

AU - Li, Meng

AU - Salvador, Jacobo

AU - Blomqvist, Sofia

AU - Runemark, Anna

AU - Kirkeby, Carsten

AU - Ignell, Rickard

AU - Bood, Joakim

AU - Brydegaard, Mikkel

N1 - Publisher Copyright: © 1995-2012 IEEE.

PY - 2022

Y1 - 2022

N2 - Insects play crucial roles in ecosystems, and how they disperse within their habitat has significant implications for our daily life. Examples include foraging ranges for pollinators, as well as the spread of disease vectors and pests. Despite technological advances with radio tags, isotopes, and genetic sequencing, insect dispersal and migration range remain challenging to study. The gold standard method of mark-recapture is tedious and inefficient. This paper demonstrates the construction of a compact, inexpensive hyperspectral fluorescence lidar. The system is based on off-the-shelf components and 3D printing. After evaluating the performance of the instrument in the laboratory, we demonstrate its efficient range-resolved fluorescence spectra in situ. We present daytime remote ranging and fluorescent identification of auto-powder-tagged honey bees. We also showcase range-, temporally- and spectrally-resolved free-flying mosquitoes, which were tagged through feeding on fluorescent-dyed sugar water. We conclude that violet light can efficiently excite administered sugar meals imbibed by flying insects. Our field experiences provide realistic expectations of signal-to-noise levels, which can be used in future studies. The technique is generally applicable and can efficiently monitor several tagged insect groups in parallel for comparative ecological analysis. This technique opens up a range of ecological experiments, which were previously unfeasible.

AB - Insects play crucial roles in ecosystems, and how they disperse within their habitat has significant implications for our daily life. Examples include foraging ranges for pollinators, as well as the spread of disease vectors and pests. Despite technological advances with radio tags, isotopes, and genetic sequencing, insect dispersal and migration range remain challenging to study. The gold standard method of mark-recapture is tedious and inefficient. This paper demonstrates the construction of a compact, inexpensive hyperspectral fluorescence lidar. The system is based on off-the-shelf components and 3D printing. After evaluating the performance of the instrument in the laboratory, we demonstrate its efficient range-resolved fluorescence spectra in situ. We present daytime remote ranging and fluorescent identification of auto-powder-tagged honey bees. We also showcase range-, temporally- and spectrally-resolved free-flying mosquitoes, which were tagged through feeding on fluorescent-dyed sugar water. We conclude that violet light can efficiently excite administered sugar meals imbibed by flying insects. Our field experiences provide realistic expectations of signal-to-noise levels, which can be used in future studies. The technique is generally applicable and can efficiently monitor several tagged insect groups in parallel for comparative ecological analysis. This technique opens up a range of ecological experiments, which were previously unfeasible.

KW - disease vectors

KW - ecology

KW - environmental monitoring

KW - fluorescence

KW - hyperspectral sensors

KW - instrumentation

KW - Laser radar

KW - pollination

KW - remote sensing

U2 - 10.1109/JSTQE.2022.3162417

DO - 10.1109/JSTQE.2022.3162417

M3 - Journal article

AN - SCOPUS:85129335875

VL - 28

JO - I E E E Journal on Selected Topics in Quantum Electronics

JF - I E E E Journal on Selected Topics in Quantum Electronics

SN - 1077-260X

IS - 5

M1 - 7100109

ER -