A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers

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Standard

A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers. / Olsaker, I.; Hellemann, A. L.; Lie, ; Jørgensen, C. B.; Thomsen, P. D.

I: Animal Genetics, Bind 24, Nr. 4, 08.1993, s. 311-313.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Olsaker, I, Hellemann, AL, Lie, , Jørgensen, CB & Thomsen, PD 1993, 'A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers', Animal Genetics, bind 24, nr. 4, s. 311-313. https://doi.org/10.1111/j.1365-2052.1993.tb00319.x

APA

Olsaker, I., Hellemann, A. L., Lie, Jørgensen, C. B., & Thomsen, P. D. (1993). A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers. Animal Genetics, 24(4), 311-313. https://doi.org/10.1111/j.1365-2052.1993.tb00319.x

Vancouver

Olsaker I, Hellemann AL, Lie , Jørgensen CB, Thomsen PD. A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers. Animal Genetics. 1993 aug.;24(4):311-313. https://doi.org/10.1111/j.1365-2052.1993.tb00319.x

Author

Olsaker, I. ; Hellemann, A. L. ; Lie, ; Jørgensen, C. B. ; Thomsen, P. D. / A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers. I: Animal Genetics. 1993 ; Bind 24, Nr. 4. s. 311-313.

Bibtex

@article{a380640b14c04e2bb1d59d14434e2794,
title = "A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers",
abstract = "A method for detecting male cells in the blood of the female calf in bovine heterosexual twin pregnancies has been established. Nucleated cells were isolated from full blood by immunomagnetic separation, lysed by boiling and then subjected to polymerase chain reaction (PCR) amplification with Y chromosome specific primers. Diagnosis was achieved within one day. The method was successfully used on blood samples that had been stored at +4°C for more than one month. Dilution of male blood in female blood showed that XY cells were detectable down to a concentration of 0.1%. This method should be amenable to automatization and can be adapted to any PCR‐based genetic test.",
keywords = "cattle, freemartin, immunomagnetic separation, Y‐specific PCR",
author = "I. Olsaker and Hellemann, {A. L.} and Lie and J{\o}rgensen, {C. B.} and Thomsen, {P. D.}",
year = "1993",
month = aug,
doi = "10.1111/j.1365-2052.1993.tb00319.x",
language = "English",
volume = "24",
pages = "311--313",
journal = "Animal Genetics",
issn = "0268-9146",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - A fast and highly sensitive method for detecting freemartinism in bovine twins using immunomagnetic beads and Y‐specific PCR primers

AU - Olsaker, I.

AU - Hellemann, A. L.

AU - Lie, null

AU - Jørgensen, C. B.

AU - Thomsen, P. D.

PY - 1993/8

Y1 - 1993/8

N2 - A method for detecting male cells in the blood of the female calf in bovine heterosexual twin pregnancies has been established. Nucleated cells were isolated from full blood by immunomagnetic separation, lysed by boiling and then subjected to polymerase chain reaction (PCR) amplification with Y chromosome specific primers. Diagnosis was achieved within one day. The method was successfully used on blood samples that had been stored at +4°C for more than one month. Dilution of male blood in female blood showed that XY cells were detectable down to a concentration of 0.1%. This method should be amenable to automatization and can be adapted to any PCR‐based genetic test.

AB - A method for detecting male cells in the blood of the female calf in bovine heterosexual twin pregnancies has been established. Nucleated cells were isolated from full blood by immunomagnetic separation, lysed by boiling and then subjected to polymerase chain reaction (PCR) amplification with Y chromosome specific primers. Diagnosis was achieved within one day. The method was successfully used on blood samples that had been stored at +4°C for more than one month. Dilution of male blood in female blood showed that XY cells were detectable down to a concentration of 0.1%. This method should be amenable to automatization and can be adapted to any PCR‐based genetic test.

KW - cattle

KW - freemartin

KW - immunomagnetic separation

KW - Y‐specific PCR

UR - http://www.scopus.com/inward/record.url?scp=0039118186&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2052.1993.tb00319.x

DO - 10.1111/j.1365-2052.1993.tb00319.x

M3 - Journal article

C2 - 8239077

AN - SCOPUS:0039118186

VL - 24

SP - 311

EP - 313

JO - Animal Genetics

JF - Animal Genetics

SN - 0268-9146

IS - 4

ER -

ID: 234883681