A Major QTL for Resistance to Vibrio anguillarum in Rainbow Trout
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A Major QTL for Resistance to Vibrio anguillarum in Rainbow Trout. / Karami, Asma M.; Ødegård, Jørgen; Marana, Moonika H.; Zuo, Shaozhi; Jaafar, Rzgar; Mathiessen, Heidi; von Gersdorff Jørgensen, Louise; Kania, Per W.; Dalsgaard, Inger; Nielsen, Torben; Buchmann, Kurt.
I: Frontiers in Genetics, Bind 11, 607558, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A Major QTL for Resistance to Vibrio anguillarum in Rainbow Trout
AU - Karami, Asma M.
AU - Ødegård, Jørgen
AU - Marana, Moonika H.
AU - Zuo, Shaozhi
AU - Jaafar, Rzgar
AU - Mathiessen, Heidi
AU - von Gersdorff Jørgensen, Louise
AU - Kania, Per W.
AU - Dalsgaard, Inger
AU - Nielsen, Torben
AU - Buchmann, Kurt
PY - 2020
Y1 - 2020
N2 - Genetic selection of disease resistant fish is a major strategy to improve health, welfare and sustainability in aquaculture. Mapping of single nucleotide polymorphisms (SNP) in the fish genome may be a fruitful tool to define relevant quantitative trait loci (QTL) and we here show its use for characterization of Vibrio anguillarum resistant rainbow trout (Oncorhynchus mykiss). Fingerlings were exposed to the pathogen V. anguillarum serotype O1 in a solution of 1.5 × 107 cfu/ml and observed for 14 days. Disease signs appeared 3 days post exposure (dpe) whereafter mortality progressed exponentially until 6 dpe reaching a total mortality of 55% within 11 days. DNA was sampled from all fish – including survivors – and analyzed on a 57 k Affymetrix SNP platform whereby it was shown that disease resistance was associated with a major QTL on chromosome 21 (Omy 21). Gene expression analyses showed that diseased fish activated genes associated with innate and adaptive immune responses. The possible genes associated with resistance are discussed.
AB - Genetic selection of disease resistant fish is a major strategy to improve health, welfare and sustainability in aquaculture. Mapping of single nucleotide polymorphisms (SNP) in the fish genome may be a fruitful tool to define relevant quantitative trait loci (QTL) and we here show its use for characterization of Vibrio anguillarum resistant rainbow trout (Oncorhynchus mykiss). Fingerlings were exposed to the pathogen V. anguillarum serotype O1 in a solution of 1.5 × 107 cfu/ml and observed for 14 days. Disease signs appeared 3 days post exposure (dpe) whereafter mortality progressed exponentially until 6 dpe reaching a total mortality of 55% within 11 days. DNA was sampled from all fish – including survivors – and analyzed on a 57 k Affymetrix SNP platform whereby it was shown that disease resistance was associated with a major QTL on chromosome 21 (Omy 21). Gene expression analyses showed that diseased fish activated genes associated with innate and adaptive immune responses. The possible genes associated with resistance are discussed.
KW - gene expression
KW - immunity
KW - QTL
KW - rainbow trout
KW - Vibrio
U2 - 10.3389/fgene.2020.607558
DO - 10.3389/fgene.2020.607558
M3 - Journal article
C2 - 33447254
AN - SCOPUS:85099357630
VL - 11
JO - Frontiers in Genetics
JF - Frontiers in Genetics
SN - 1664-8021
M1 - 607558
ER -
ID: 256986844