A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells. / Xie, Jiexiong; Trus, Ivan; Oh, Dayoung; Kvisgaard, Lise K.; Rappe, Julie C.F.; Ruggli, Nicolas; Vanderheijden, Nathalie; Larsen, Lars E.; Lefèvre, François; Nauwynck, Hans J.

I: Viruses, Bind 11, Nr. 1, 36, 01.2019.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Xie, J, Trus, I, Oh, D, Kvisgaard, LK, Rappe, JCF, Ruggli, N, Vanderheijden, N, Larsen, LE, Lefèvre, F & Nauwynck, HJ 2019, 'A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells', Viruses, bind 11, nr. 1, 36. https://doi.org/10.3390/v11010036

APA

Xie, J., Trus, I., Oh, D., Kvisgaard, L. K., Rappe, J. C. F., Ruggli, N., Vanderheijden, N., Larsen, L. E., Lefèvre, F., & Nauwynck, H. J. (2019). A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells. Viruses, 11(1), [36]. https://doi.org/10.3390/v11010036

Vancouver

Xie J, Trus I, Oh D, Kvisgaard LK, Rappe JCF, Ruggli N o.a. A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells. Viruses. 2019 jan.;11(1). 36. https://doi.org/10.3390/v11010036

Author

Xie, Jiexiong ; Trus, Ivan ; Oh, Dayoung ; Kvisgaard, Lise K. ; Rappe, Julie C.F. ; Ruggli, Nicolas ; Vanderheijden, Nathalie ; Larsen, Lars E. ; Lefèvre, François ; Nauwynck, Hans J. / A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells. I: Viruses. 2019 ; Bind 11, Nr. 1.

Bibtex

@article{c2b6fd2113184c8aa087de9655e18a74,
title = "A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells",
abstract = "The Meat Animal Research Center-145 (MARC-145) cell line has been proven to be valuable for viral attenuation regarding vaccine development and production. Cell-adaptation is necessary for the efficient replication of porcine reproductive and respiratory syndrome virus (PRRSV) in these cells. Multiple sequence analysis revealed consistent amino acid substitutions in GP2a (V88F, M94I, F95L) of MARC-145 cell-adapted strains. To investigate the putative effect of these substitutions, mutations at either position 88, 94, 95, and their combinations were introduced into two PRRSV1 (13V091 and IVI-1173) infectious clones followed by the recovery of viable recombinants. When comparing the replication kinetics in MARC-145 cells, a strongly positive effect on the growth characteristics of the 13V091 strain (+2.1 log10) and the IVI-1173 strain (+1.7 log10) compared to wild-type (WT) virus was only observed upon triple amino acid substitution at positions 88 (V88F), 94 (M94I), and 95 (F95L) of GP2a, suggesting that the triple mutation is a determining factor in PRRSV1 adaptation to MARC-145 cells.",
keywords = "Adaptation mutation, GP2a, MARC-145, PRRSV",
author = "Jiexiong Xie and Ivan Trus and Dayoung Oh and Kvisgaard, {Lise K.} and Rappe, {Julie C.F.} and Nicolas Ruggli and Nathalie Vanderheijden and Larsen, {Lars E.} and Fran{\c c}ois Lef{\`e}vre and Nauwynck, {Hans J.}",
year = "2019",
month = jan,
doi = "10.3390/v11010036",
language = "English",
volume = "11",
journal = "Viruses",
issn = "1999-4915",
publisher = "M D P I AG",
number = "1",

}

RIS

TY - JOUR

T1 - A triple amino acid substitution at position 88/94/95 in glycoprotein gp2a of type 1 porcine reproductive and respiratory syndrome virus (Prrsv1) is responsible for adaptation to marc-145 cells

AU - Xie, Jiexiong

AU - Trus, Ivan

AU - Oh, Dayoung

AU - Kvisgaard, Lise K.

AU - Rappe, Julie C.F.

AU - Ruggli, Nicolas

AU - Vanderheijden, Nathalie

AU - Larsen, Lars E.

AU - Lefèvre, François

AU - Nauwynck, Hans J.

PY - 2019/1

Y1 - 2019/1

N2 - The Meat Animal Research Center-145 (MARC-145) cell line has been proven to be valuable for viral attenuation regarding vaccine development and production. Cell-adaptation is necessary for the efficient replication of porcine reproductive and respiratory syndrome virus (PRRSV) in these cells. Multiple sequence analysis revealed consistent amino acid substitutions in GP2a (V88F, M94I, F95L) of MARC-145 cell-adapted strains. To investigate the putative effect of these substitutions, mutations at either position 88, 94, 95, and their combinations were introduced into two PRRSV1 (13V091 and IVI-1173) infectious clones followed by the recovery of viable recombinants. When comparing the replication kinetics in MARC-145 cells, a strongly positive effect on the growth characteristics of the 13V091 strain (+2.1 log10) and the IVI-1173 strain (+1.7 log10) compared to wild-type (WT) virus was only observed upon triple amino acid substitution at positions 88 (V88F), 94 (M94I), and 95 (F95L) of GP2a, suggesting that the triple mutation is a determining factor in PRRSV1 adaptation to MARC-145 cells.

AB - The Meat Animal Research Center-145 (MARC-145) cell line has been proven to be valuable for viral attenuation regarding vaccine development and production. Cell-adaptation is necessary for the efficient replication of porcine reproductive and respiratory syndrome virus (PRRSV) in these cells. Multiple sequence analysis revealed consistent amino acid substitutions in GP2a (V88F, M94I, F95L) of MARC-145 cell-adapted strains. To investigate the putative effect of these substitutions, mutations at either position 88, 94, 95, and their combinations were introduced into two PRRSV1 (13V091 and IVI-1173) infectious clones followed by the recovery of viable recombinants. When comparing the replication kinetics in MARC-145 cells, a strongly positive effect on the growth characteristics of the 13V091 strain (+2.1 log10) and the IVI-1173 strain (+1.7 log10) compared to wild-type (WT) virus was only observed upon triple amino acid substitution at positions 88 (V88F), 94 (M94I), and 95 (F95L) of GP2a, suggesting that the triple mutation is a determining factor in PRRSV1 adaptation to MARC-145 cells.

KW - Adaptation mutation

KW - GP2a

KW - MARC-145

KW - PRRSV

U2 - 10.3390/v11010036

DO - 10.3390/v11010036

M3 - Journal article

C2 - 30626009

AN - SCOPUS:85059796478

VL - 11

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 1

M1 - 36

ER -

ID: 247392976