A zebrafish model to elucidate the impact of host genes on the microbiota
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A zebrafish model to elucidate the impact of host genes on the microbiota. / Thormar, Eiríkur Andri; Rasmussen, Jacob Agerbo; Mathiessen, Heidi; Marana, Moonika Haahr; Clausen, Cecilie Grønlund; Hansen, Martin; Kodama, Miyako; von Gersdorff Jørgensen, Louise; Limborg, Morten Tønsberg.
I: Environmental DNA, Bind 6, Nr. 1, e513, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - A zebrafish model to elucidate the impact of host genes on the microbiota
AU - Thormar, Eiríkur Andri
AU - Rasmussen, Jacob Agerbo
AU - Mathiessen, Heidi
AU - Marana, Moonika Haahr
AU - Clausen, Cecilie Grønlund
AU - Hansen, Martin
AU - Kodama, Miyako
AU - von Gersdorff Jørgensen, Louise
AU - Limborg, Morten Tønsberg
PY - 2024
Y1 - 2024
N2 - Every host species and organism provides a unique environmental niche contributing to the overall diversity of microbial ecosystems from the intestine of an animal to the oceans and forests of our planet. The study of host–microbiota interactions has long focused on the well-established effects the microbiota has on its host. In contrast, little focus has been allocated to the role of the host in these intricate interactions. However, understanding the role of the host may well be an essential key to understanding the complexity of the relationship between the host and its microbiota. In this study, we present a model in which the effects of host genes on the microbiota can be elucidated and how such genetic effects may shape host-associated microbiota. We demonstrate a hologenomic approach implementing the CRISPR/Cas system in the zebrafish model to combine the effects of a host gene with 16S metabarcoding and metabolomics data. We show that knocking out the gene coding for the rate-limiting enzyme in melanogenesis, tyrosinase (tyr), correlates with changes in the intestinal microbiota of zebrafish and differences in the abundance of specific metabolites illustrating the value of our model for studying the impact of host genes on the composition and function of the intestinal microbiota.
AB - Every host species and organism provides a unique environmental niche contributing to the overall diversity of microbial ecosystems from the intestine of an animal to the oceans and forests of our planet. The study of host–microbiota interactions has long focused on the well-established effects the microbiota has on its host. In contrast, little focus has been allocated to the role of the host in these intricate interactions. However, understanding the role of the host may well be an essential key to understanding the complexity of the relationship between the host and its microbiota. In this study, we present a model in which the effects of host genes on the microbiota can be elucidated and how such genetic effects may shape host-associated microbiota. We demonstrate a hologenomic approach implementing the CRISPR/Cas system in the zebrafish model to combine the effects of a host gene with 16S metabarcoding and metabolomics data. We show that knocking out the gene coding for the rate-limiting enzyme in melanogenesis, tyrosinase (tyr), correlates with changes in the intestinal microbiota of zebrafish and differences in the abundance of specific metabolites illustrating the value of our model for studying the impact of host genes on the composition and function of the intestinal microbiota.
U2 - 10.1002/edn3.513
DO - 10.1002/edn3.513
M3 - Journal article
VL - 6
JO - Environmental DNA
JF - Environmental DNA
SN - 2637-4943
IS - 1
M1 - e513
ER -
ID: 382156685