Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis
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Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis. / Titze, Isabel; Krömker, Volker.
I: Veterinary Sciences, Bind 7, Nr. 1, 31, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis
AU - Titze, Isabel
AU - Krömker, Volker
PY - 2020
Y1 - 2020
N2 - The antimicrobial activity of a phagemixture and a lactic acid bacteriumagainst Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1. ST29, EB1. ST11 and EB1. ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.
AB - The antimicrobial activity of a phagemixture and a lactic acid bacteriumagainst Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1. ST29, EB1. ST11 and EB1. ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.
KW - Bacteriophage mixture
KW - Bovine mastitis
KW - Dairy
KW - Lactic acid bacterium
KW - Lytic phage
KW - Phage therapy
KW - Staphylococcus aureus
U2 - 10.3390/vetsci7010031
DO - 10.3390/vetsci7010031
M3 - Journal article
C2 - 32155751
AN - SCOPUS:85081919379
VL - 7
JO - Veterinary Sciences
JF - Veterinary Sciences
SN - 2306-7381
IS - 1
M1 - 31
ER -
ID: 239014795