Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates. / Moura, M. C.; Procópio, T. F.; Ferreira, G. R.S.; Alves, R. R.V.; Sá, R. A.; Paiva, P. M.G.; Ingmer, H.; Coelho, L. C.B.B.; Napoleão, T. H.
I: Journal of Applied Microbiology, Bind 130, Nr. 3, 2021, s. 745-754.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Anti-staphylococcal effects of Myracrodruon urundeuva lectins on nonresistant and multidrug resistant isolates
AU - Moura, M. C.
AU - Procópio, T. F.
AU - Ferreira, G. R.S.
AU - Alves, R. R.V.
AU - Sá, R. A.
AU - Paiva, P. M.G.
AU - Ingmer, H.
AU - Coelho, L. C.B.B.
AU - Napoleão, T. H.
PY - 2021
Y1 - 2021
N2 - Aims: To evaluate the anti-staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) of Myracrodruon urundeuva. Methods and Results: The lectins were evaluated for: effects on growth, aggregation, haemolytic activity and biofilm-forming ability of Staphylococcus aureus clinical isolates nonresistant (8325-4) and multidrug resistant (LAC USA300); interference with the expression of virulence genes (hla, rnaIII and spa) of the Agr system of S. aureus; and synergistic effect with the antibiotics cefoxitin and cefotaxime. MuBL, MuHL and MuLL reduced growth (minimal inhibitory concentration (MIC): 12·5–50 µg ml−1) and viability (minimal bactericidal concentration (MBC): 100 µg ml−1) of 8325-4 and LAC USA300 cells. MuLL (at ½MIC and MIC) reduced LAC USA300 agglutination. The lectins did not interfere with haemolytic activity and expression of hla, rnaIII and spa genes. Only MuHL was able to reduce the biofilm production by 8325-4 (50–400 µg ml−1) and LAC USA300 (400 µg ml−1). Conclusion: The M. urundeuva lectins showed antibacterial activity against nonresistant and resistant clinical isolates of S. aureus and synergistic effects with antibiotics in reducing growth and biofilm formation. Significance and Impact of the Study: This work reports bioactive molecules capable of acting as anti-staphylococcal agents, since there are increasing reports of multiresistant isolates of this bacterium.
AB - Aims: To evaluate the anti-staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) of Myracrodruon urundeuva. Methods and Results: The lectins were evaluated for: effects on growth, aggregation, haemolytic activity and biofilm-forming ability of Staphylococcus aureus clinical isolates nonresistant (8325-4) and multidrug resistant (LAC USA300); interference with the expression of virulence genes (hla, rnaIII and spa) of the Agr system of S. aureus; and synergistic effect with the antibiotics cefoxitin and cefotaxime. MuBL, MuHL and MuLL reduced growth (minimal inhibitory concentration (MIC): 12·5–50 µg ml−1) and viability (minimal bactericidal concentration (MBC): 100 µg ml−1) of 8325-4 and LAC USA300 cells. MuLL (at ½MIC and MIC) reduced LAC USA300 agglutination. The lectins did not interfere with haemolytic activity and expression of hla, rnaIII and spa genes. Only MuHL was able to reduce the biofilm production by 8325-4 (50–400 µg ml−1) and LAC USA300 (400 µg ml−1). Conclusion: The M. urundeuva lectins showed antibacterial activity against nonresistant and resistant clinical isolates of S. aureus and synergistic effects with antibiotics in reducing growth and biofilm formation. Significance and Impact of the Study: This work reports bioactive molecules capable of acting as anti-staphylococcal agents, since there are increasing reports of multiresistant isolates of this bacterium.
KW - antibacterial activity
KW - lectin
KW - multidrug resistant
KW - Staphylococcus aureus
KW - synergistic effects
KW - virulence factors
U2 - 10.1111/jam.14811
DO - 10.1111/jam.14811
M3 - Journal article
C2 - 32750211
AN - SCOPUS:85089379590
VL - 130
SP - 745
EP - 754
JO - Proceedings of the Society for Applied Bacteriology
JF - Proceedings of the Society for Applied Bacteriology
SN - 0370-1778
IS - 3
ER -
ID: 247339664