Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy
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Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy. / Brogaard, Louise; Lyngby, Janne G.; Kristensen, Annemarie T.; Fredholm, Merete; Bjørnvad, Charlotte R.; Salavati Schmitz, Silke; Skancke, Ellen; Morris, Joanna S.; Dupont, Nana; Argyle, David; Sánchez, Armand; Spohr, Anette; Graarup-Hansen, Kasper; Nielsen, Lise N.; Cirera, Susanna.
I: Journal of Veterinary Internal Medicine, Bind 37, Nr. 5, 2023, s. 1738-1749.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy
AU - Brogaard, Louise
AU - Lyngby, Janne G.
AU - Kristensen, Annemarie T.
AU - Fredholm, Merete
AU - Bjørnvad, Charlotte R.
AU - Salavati Schmitz, Silke
AU - Skancke, Ellen
AU - Morris, Joanna S.
AU - Dupont, Nana
AU - Argyle, David
AU - Sánchez, Armand
AU - Spohr, Anette
AU - Graarup-Hansen, Kasper
AU - Nielsen, Lise N.
AU - Cirera, Susanna
N1 - © 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
PY - 2023
Y1 - 2023
N2 - BACKGROUND: Differentiation of gastrointestinal cancer (GIC) from chronic inflammatory enteropathies (CIE) in cats can be challenging and often requires extensive diagnostic testing. MicroRNAs (miRNAs) have promise as non-invasive biomarkers in serum and feces for diagnosis of GIC.HYPOTHESIS/OBJECTIVES: Cats with GIC will have serum and fecal miRNA profiles that differ significantly from healthy cats and cats with CIE. Identify serum and fecal miRNAs with diagnostic potential for differentiation between cats with GIC and CIE as compared to healthy cats.ANIMALS: Ten healthy cats, 9 cats with CIE, and 10 cats with GIC; all client-owned.METHODS: Cats were recruited for an international multicenter observational prospective case-control study. Serum and feces were screened using small RNA sequencing for miRNAs that differed in abundance between cats with GIC and CIE, and healthy cats. Diagnostic biomarker potential of relevant miRNAs from small RNA sequencing and the literature was confirmed using reverse transcription quantitative real-time PCR (RT-qPCR).RESULTS: Serum miR-223-3p was found to distinguish between cats with GIC and CIE with an area under the curve (AUC) of 0.9 (95% confidence interval [CI], 0.760-1.0), sensitivity of 90% (95% CI, 59.6-99.5%), and specificity of 77.8% (95% CI, 45.3-96.1%). Serum miR-223-3p likewise showed promise in differentiating a subgroup of cats with small cell lymphoma (SCL) from those with CIE. No fecal miRNAs could distinguish between cats with GIC and CIE.CONCLUSION AND CLINICAL IMPORTANCE: Serum miR-223-3p potentially may serve as a noninvasive diagnostic biomarker of GIC in cats, in addition to providing a much needed tool for the differentiation of CIE and SCL.
AB - BACKGROUND: Differentiation of gastrointestinal cancer (GIC) from chronic inflammatory enteropathies (CIE) in cats can be challenging and often requires extensive diagnostic testing. MicroRNAs (miRNAs) have promise as non-invasive biomarkers in serum and feces for diagnosis of GIC.HYPOTHESIS/OBJECTIVES: Cats with GIC will have serum and fecal miRNA profiles that differ significantly from healthy cats and cats with CIE. Identify serum and fecal miRNAs with diagnostic potential for differentiation between cats with GIC and CIE as compared to healthy cats.ANIMALS: Ten healthy cats, 9 cats with CIE, and 10 cats with GIC; all client-owned.METHODS: Cats were recruited for an international multicenter observational prospective case-control study. Serum and feces were screened using small RNA sequencing for miRNAs that differed in abundance between cats with GIC and CIE, and healthy cats. Diagnostic biomarker potential of relevant miRNAs from small RNA sequencing and the literature was confirmed using reverse transcription quantitative real-time PCR (RT-qPCR).RESULTS: Serum miR-223-3p was found to distinguish between cats with GIC and CIE with an area under the curve (AUC) of 0.9 (95% confidence interval [CI], 0.760-1.0), sensitivity of 90% (95% CI, 59.6-99.5%), and specificity of 77.8% (95% CI, 45.3-96.1%). Serum miR-223-3p likewise showed promise in differentiating a subgroup of cats with small cell lymphoma (SCL) from those with CIE. No fecal miRNAs could distinguish between cats with GIC and CIE.CONCLUSION AND CLINICAL IMPORTANCE: Serum miR-223-3p potentially may serve as a noninvasive diagnostic biomarker of GIC in cats, in addition to providing a much needed tool for the differentiation of CIE and SCL.
U2 - 10.1111/jvim.16813
DO - 10.1111/jvim.16813
M3 - Journal article
C2 - 37486176
VL - 37
SP - 1738
EP - 1749
JO - Journal of Veterinary Internal Medicine
JF - Journal of Veterinary Internal Medicine
SN - 0891-6640
IS - 5
ER -
ID: 361030535