Bovine cumulus-oocyte disconnection in vitro

Publikation: Bidrag til tidsskriftTidsskriftartikel

Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.
OriginalsprogEngelsk
TidsskriftBrain Structure and Function (Print Edition)
Vol/bind176
Sider (fra-til)41-44
Antal sider4
ISSN1863-2653
StatusUdgivet - 1987

ID: 8067538