Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds

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Standard

Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. / Nielsen, S. S.; Thamsborg, S. M.; Houe, H.; Bitsch, V.

I: Preventive Veterinary Medicine, Bind 44, Nr. 1-2, 29.03.2000, s. 1-7.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nielsen, SS, Thamsborg, SM, Houe, H & Bitsch, V 2000, 'Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds', Preventive Veterinary Medicine, bind 44, nr. 1-2, s. 1-7. https://doi.org/10.1016/S0167-5877(00)00098-2

APA

Nielsen, S. S., Thamsborg, S. M., Houe, H., & Bitsch, V. (2000). Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. Preventive Veterinary Medicine, 44(1-2), 1-7. https://doi.org/10.1016/S0167-5877(00)00098-2

Vancouver

Nielsen SS, Thamsborg SM, Houe H, Bitsch V. Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. Preventive Veterinary Medicine. 2000 mar. 29;44(1-2):1-7. https://doi.org/10.1016/S0167-5877(00)00098-2

Author

Nielsen, S. S. ; Thamsborg, S. M. ; Houe, H. ; Bitsch, V. / Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. I: Preventive Veterinary Medicine. 2000 ; Bind 44, Nr. 1-2. s. 1-7.

Bibtex

@article{4fd1c206409c410b84cb53892062460c,
title = "Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds",
abstract = "Paratuberculosis (Johne's disease) has been widespread in Danish dairy herds for a long time but the herd-level prevalence has never been determined precisely. To evaluate the prevalence of paratuberculosis in Danish dairy herds in various regions, an ELISA based on a commercially available antigen was adapted for testing bulk-tank milk for the presence of antibodies to Mycobacterium avium subsp. paratuberculosis. Bulk-tank milk samples were collected from six milk-collecting centres from six different areas of the country. Samples from 900 herds (about 7.5% of all Danish dairy herds) were examined, and 70% were positive at the statistically optimal cut-off (sensitivity 97.1%; specificity 83.3%). The technical performance of the ELISA was not sufficient to provide a tool for surveillance because even slight changes in optical density for the samples would change the classification of some samples. The infection is more widespread than previous investigations have shown. (C) 2000 Elsevier Science B.V.",
keywords = "Bulk-tank milk, Cattle-microbiological diseases, ELISA, Johne's disease, Mycobacterium avium subsp. paratuberculosis",
author = "Nielsen, {S. S.} and Thamsborg, {S. M.} and H. Houe and V. Bitsch",
year = "2000",
month = mar,
day = "29",
doi = "10.1016/S0167-5877(00)00098-2",
language = "English",
volume = "44",
pages = "1--7",
journal = "Preventive Veterinary Medicine",
issn = "0167-5877",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds

AU - Nielsen, S. S.

AU - Thamsborg, S. M.

AU - Houe, H.

AU - Bitsch, V.

PY - 2000/3/29

Y1 - 2000/3/29

N2 - Paratuberculosis (Johne's disease) has been widespread in Danish dairy herds for a long time but the herd-level prevalence has never been determined precisely. To evaluate the prevalence of paratuberculosis in Danish dairy herds in various regions, an ELISA based on a commercially available antigen was adapted for testing bulk-tank milk for the presence of antibodies to Mycobacterium avium subsp. paratuberculosis. Bulk-tank milk samples were collected from six milk-collecting centres from six different areas of the country. Samples from 900 herds (about 7.5% of all Danish dairy herds) were examined, and 70% were positive at the statistically optimal cut-off (sensitivity 97.1%; specificity 83.3%). The technical performance of the ELISA was not sufficient to provide a tool for surveillance because even slight changes in optical density for the samples would change the classification of some samples. The infection is more widespread than previous investigations have shown. (C) 2000 Elsevier Science B.V.

AB - Paratuberculosis (Johne's disease) has been widespread in Danish dairy herds for a long time but the herd-level prevalence has never been determined precisely. To evaluate the prevalence of paratuberculosis in Danish dairy herds in various regions, an ELISA based on a commercially available antigen was adapted for testing bulk-tank milk for the presence of antibodies to Mycobacterium avium subsp. paratuberculosis. Bulk-tank milk samples were collected from six milk-collecting centres from six different areas of the country. Samples from 900 herds (about 7.5% of all Danish dairy herds) were examined, and 70% were positive at the statistically optimal cut-off (sensitivity 97.1%; specificity 83.3%). The technical performance of the ELISA was not sufficient to provide a tool for surveillance because even slight changes in optical density for the samples would change the classification of some samples. The infection is more widespread than previous investigations have shown. (C) 2000 Elsevier Science B.V.

KW - Bulk-tank milk

KW - Cattle-microbiological diseases

KW - ELISA

KW - Johne's disease

KW - Mycobacterium avium subsp. paratuberculosis

UR - http://www.scopus.com/inward/record.url?scp=0034728624&partnerID=8YFLogxK

U2 - 10.1016/S0167-5877(00)00098-2

DO - 10.1016/S0167-5877(00)00098-2

M3 - Journal article

C2 - 10727740

AN - SCOPUS:0034728624

VL - 44

SP - 1

EP - 7

JO - Preventive Veterinary Medicine

JF - Preventive Veterinary Medicine

SN - 0167-5877

IS - 1-2

ER -

ID: 200664323