CEREBROSPINAL FLUID FROM PRETERM PIGS WITH NECROTIZING ENTEROCOLITIS HAS ALTERED CYTOKINE PROFILE AND PROMOTES HIPPOCAMPAL NEURITOGENESIS
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CEREBROSPINAL FLUID FROM PRETERM PIGS WITH NECROTIZING ENTEROCOLITIS HAS ALTERED CYTOKINE PROFILE AND PROMOTES HIPPOCAMPAL NEURITOGENESIS. / Pankratova, Stanislava; Sun, J.; Li, Y.; Sangild, Per Torp.
I: European Journal of Pediatrics, Bind 175, Nr. 11, EAPS-0806, 11.2016, s. 1688-1688.Publikation: Bidrag til tidsskrift › Konferenceabstrakt i tidsskrift › Forskning › fagfællebedømt
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TY - ABST
T1 - CEREBROSPINAL FLUID FROM PRETERM PIGS WITH NECROTIZING ENTEROCOLITIS HAS ALTERED CYTOKINE PROFILE AND PROMOTES HIPPOCAMPAL NEURITOGENESIS
AU - Pankratova, Stanislava
AU - Sun, J.
AU - Li, Y.
AU - Sangild, Per Torp
N1 - Conference code: 6
PY - 2016/11
Y1 - 2016/11
N2 - Background and aimsNecrotizing enterocolitis (NEC) in preterm infants is associated withneurodevelopmental delay and cerebral palsy. We hypothesized that intestinalNEC lesions affect inflammatory cytokines in cerebrospinal fluid(CSF) which in turn may affect neurite differentiation.MethodsVariable degrees of NEC lesions developed spontaneously in pigletsreared for 9 days after preterm birth. CSF samples were collected andcytokine profile was evaluated by multiplex cytokine array ELISA.Hippocampal structures were dissected and cytokine mRNAs were measuredby qPCR. The ability of piglet CSF to promote neurite outgrowthwas quantified by stereology using primary rat hippocampal neuronsin vitro.ResultsRelative to healthy controls, pigs with NEC lesions in either intestine orcolon showed altered concentrations for 7 of 32 cytokines in CSF andhigher neurite outgrowth in the in vitro neuronal differentiation model.The qPCR analyses showed higher erythropoietin mRNA in the hippocampusof NEC pigs.ConclusionsIntestinal NEC lesions affect CSF cytokine profile in preterm pigs andthis may affect the differentiation of neurons in the brain. Neuronal plasticityin immature brain may explain that NEC lesions, via changes inCSF cytokine levels, may affect neurodevelopment in preterm neonates
AB - Background and aimsNecrotizing enterocolitis (NEC) in preterm infants is associated withneurodevelopmental delay and cerebral palsy. We hypothesized that intestinalNEC lesions affect inflammatory cytokines in cerebrospinal fluid(CSF) which in turn may affect neurite differentiation.MethodsVariable degrees of NEC lesions developed spontaneously in pigletsreared for 9 days after preterm birth. CSF samples were collected andcytokine profile was evaluated by multiplex cytokine array ELISA.Hippocampal structures were dissected and cytokine mRNAs were measuredby qPCR. The ability of piglet CSF to promote neurite outgrowthwas quantified by stereology using primary rat hippocampal neuronsin vitro.ResultsRelative to healthy controls, pigs with NEC lesions in either intestine orcolon showed altered concentrations for 7 of 32 cytokines in CSF andhigher neurite outgrowth in the in vitro neuronal differentiation model.The qPCR analyses showed higher erythropoietin mRNA in the hippocampusof NEC pigs.ConclusionsIntestinal NEC lesions affect CSF cytokine profile in preterm pigs andthis may affect the differentiation of neurons in the brain. Neuronal plasticityin immature brain may explain that NEC lesions, via changes inCSF cytokine levels, may affect neurodevelopment in preterm neonates
U2 - 10.1007/s00431-016-2785-8
DO - 10.1007/s00431-016-2785-8
M3 - Conference abstract in journal
C2 - 27744567
VL - 175
SP - 1688
EP - 1688
JO - Acta Paediatrica Hungarica
JF - Acta Paediatrica Hungarica
SN - 0340-6199
IS - 11
M1 - EAPS-0806
Y2 - 21 October 2016 through 25 October 2016
ER -
ID: 172393704